CLONING: NEW FRONTIERS OF ANIMAL REPRODUCTIVE BIOTECHNOLOGY - PowerPoint Presentation

Slide1

CLONING: NEW FRONTIERS OF ANIMAL REPRODUCTIVE BIOTECHNOLOGY

Dr.

Gatot

CiptadiLab. Of Genetics and Animal BreedingFac of Animal Sci., UBLSIH-UB

BIOTEKNOLOGI REPRODUKSI

MAMPU MENYELAMATKAN

SUMBER GENETIK HEWAN LANGKA

GT

Introduction

somatis

oosit

sperma

Basic technique for nuclear transfer

(cloning)

1. Cryopreservation (Freezing) Seeding, Stepwise, Direct, Vitrification

2. Embryo transfer (ET)

Superovulation

Collection of embryos (Flushing)

Evaluation of embryos

Synchronization of estrus

3. In vitro fertilization (IVF)

Collection of

oocytes

from ovaries

In vitro maturation

In vitro fertilization

In vitro culture

4. Micromanipulation

Sperminjection

(ICSI; IVM, Activation, IVC) Clone (Nuclear transfer) IVM, IVF, IVC Enucleation, Nuclear injection Fusion, Activation Gene transfer ( vector, Electropolation) Gene transfer (Injection into zygotes)

Cloning Techniques

Desection of morula and blastocyst (Embryo)

Deaggregation (Isolation) of blastomeres (Embryo) Clone

Micromanipulation

Nuclear transfer

Micromanipulation +Gene transfer

1. Desection of morula and blastocyst (Embryo)

2. Deaggregation (Isolation) of blastomeres (Embryo)

DREAMS SOMETIMES COME TRUE !

Bioteghnology has the possibility to be revived a scare and an extinct animal (Film Jurasic Park)

The birth of the clone animals is great influencing the

frontier of research of animal reproductive biotechnologyWhy ?Somatic cell (deferentiated

) was

reprogram

ed

back to

embr

y

onal

stage and resulted in normal offsprings

.

GT

Bioteknologi

Reproduksi: Sumbangan

KLONING

Pada

Ilmu

Pengetahuan

GT

Nuclear transfer

Activation

ET

Invitro maturation

Surrogate mother pig

S u m m a r y

Recipient oocytes

Donor cell

Culture of Cloned embryos

In vitro culture

Enucleation

Introduction of donor cell

Enucleation

Passages of somatic cells

Activation

Enbryo transfer

Production of transgenic piglets

In vitro Maturation

of oocytes

Introduction of

Extraneous gene

Xenotransplatation

Production of

human protein

Synchronization

of estrus

Surrogate mother of miniature pigs

Nuclear trasfer

Recipient oocytes

Donor cell derived from miniature pigs

Selection of M

2

Nuclear transfer procedure to produce

reconstructed embryo: inject donor cell in the

perivittelin space of enuclated oocyte

the performed fusion cell is performed intracytoplasmic direct nuclear injection (IDNI) activated artificially

Enucleated

Non enucleated

IDNI

Enucleation

TECHNICAL ASPECT: TN

1

2

3

4

A

B

Sel

Hidup…

/

Mati…

1. Besar,

2. Sedang,

3. Kecil: 22.16 %

4.AbnormalS e l e k s i S e l D o n o r

Tanpa Aktivasi

Aktivasi tunggal

Ukuran sel

DEVELOPMENT OF NEW TECH. IN REPROD ANIMAL

1

1997

Birth of dolly (1

st

animal cloning using somatic cells)

2

1998

Birth of cows: Charlie/George:

( serum albumin)

Specific protein for human blood aglutination

3

2000

Pig cloning (transgenic) for organ transplantation (human),

4.

2001

Inter species nuclear

trasnfer

(Yak to

Bos

Taurus)

advantages: non conventional product of livestock

:

Genetic quality improvement

Biopharmacies

Organ Transplantation (Biomedic industry)

Genetic concervation of endangered animals

Genetic

Consideration and Constraints of Gen Manipulation???:

No.

Persilangan

Fertilitas

Keunggulan F1

Jantan

Betina

1.

BantengX Zebu

-

+

Laju pertumbuhan

2.

Keledai Xkuda

-

+

Kerja

+

ketahanan

3.

Sapi x Yak

-

-

BB/Power/

Susu

4.

Bos taurusxB. Indicus

+

+

Daging

5.

Entog X Itik

?

?

Daging

/

pertb

.

Catatan:

Bukan penghasil bibit

Secara teknis memungkinkan, tetapi

Tujuan utama bukan genetik .

Have a nice dream

………

Dr. Tanaka H.

CONCLUSSION

:

Result from IVM, IVF, IVG of germ cell are promising

What Further research: ?