Lab 4 Osmotic pressure The Osmolar concentration mainly used in biology to explain the - PowerPoint Presentation

Slide1

Lab 4Osmotic pressure

The Osmolar concentration mainly used in biology to explain the osmotic effect, We suggest the existence of a container divided by water permeable membrane to two sides A and B . water presence in both sides and glucose (1 M) in side B

only

Osmolar Solution

So to reaching equilibrate stage the water will move from (A to B) { from high water concentration to low water concentration } . This movement of water called

Osmosis

, and the power that mated on the membrane called

Osmotic

pressure

Osmolar Solution

H2O





A

1M Glucose

1

Osm

B

Osmolar Solution

●

Osmol

(

Osm

)

:-A unite used to explain the capability of a solution to make Osmotic pressure.

◙ Every (1 m) of glucose sheds osmotic pressure on membrane about (1

Osm

) this applies to all

non ionic

molecules

.So

i

f we put (2M) of glucose in the side B this will cause ↑ in water movement from A side to B side causing ↑ in Osmotic pressure.

Osmolar Solution

◙About the

ionic molecules

such as

Nacl

, when we dissolve

Nacl

in water

Nacl

molecules will break down to 2 ions .

Nacl

------------> 1 Na + 1

cl

1mole

---------> 1mol + 1mole = 2mole

Each ion will cause osmotic pressure of it′s on , so the total osmotic pressure equal to the summation of the osmotic pressure of all the ions in the solution.

Osmolar Solution

H2O









A

(1 Na + 1

cl

)

2M

2

Osm

B

Osmolar Solution◙ Osmotic pressure of all mammals cell is 0.3

Osm

{300

mOsm

} milliosmolar , if we put normal cell in solution of

0.3

Osm

of glucose .

Or 0.15

Osm

of

Nacl

This cell will be intact , this solution called

isotonic

solution

.

Osmolar Solution

Isotonic Solution

:- A solution with osmotic pressure equal to osmotic pressure inside mammals’ cells. {the cell remain intact}

Hypertonic Solution

:- A solution with osmotic pressure higher than the osmotic pressure inside the cell. {the cell will be Shrinks }

Hypotonic

Solution

:- A solution with osmotic pressure lower than the osmotic pressure inside the cell .{the cell will be destroyed }

Osmolar Solution

Ex } prepare 1

Osm

solution of

Nacl

,not that mw of

Nacl

is 58.5 .

Nacl

------------> 1 Na + 1

cl

58.5

÷ 2 = 29.5 gm /1 L

Ex} prepare 1

Osm

of Cacl­

2

solution , mw 110.

Cacl­

2

--------- > 1 Ca + 2

cl

110

÷ 3 = 36.6 gm /1 L

Ex } prepare 1

Osm

of glucose ,mw 180.