hM3D Gq is Gq coupled DREADD It is derived from human muscarinic receptor M3 sequence with two point mutations Y149C A239G hM 4 D Gi is Gi coupled DREADD It is derived from human ID: 931533
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Two point mutations convert native receptor into DREADD
hM3D(Gq) is Gq coupled DREADD. It is derived from human muscarinic receptor M3 sequence with two point mutations (Y149C, A239G)hM4D(Gi) is Gi coupled DREADD. It is derived from human muscarinic receptor M4 sequence with two point mutations (Y113C, A203G) rM3D(Gs) is Gs coupled DREADD. It is derived from rat muscarinic receptor M3 sequence with replacement of i2 and i3 loop with turkey β1 AR sequence. It also contains two point mutations (Y148C, A238G).