Advanced electron microscopy reveals enormous variation in shape density and dimensions of trichomes in Solanaceae Background Trichomes Glandular Nonglandular Image credits Sakshi Watts ID: 931231
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Slide1
Sakshi WattsGraduate Assistant
Advanced electron microscopy reveals enormous variation in shape, density, and dimensions of
trichomes in Solanaceae
Slide2Background: Trichomes
Glandular
Non-glandular
Image credits: Sakshi Watts
2
Slide3Background: Role of trichomes in plant defense
Deter herbivory
Post-ingestive damage
Video credits: Dr. Rupesh Kariyat
Picture source: Kaur et al. 2021
Kariyat et al., 2017
Image and video credits: Dr. Rupesh Kariyat and Annette Diaz
3
Slide4Knowledge gapsInter and intra generic variation of trichomesInter and intra specific variation
4
Slide5Materials: Solanum as plant study system
S. lycopersicum
(tomato)
S. melanocerasum
(huckleberry)
S. anguivi
(forest bitterberry)
S. pyracanthos
(porcupine tomato)
S. macrocarpon
(African eggplant)
S. dulcamara
(bittersweet nightshade)
S. lanceifolium
(lance-leaved n.)
S. aethiopicum
(Ethiopian eggplant)
5
Slide6S. elaeagnifolium
(Silverleaf nightshade)
S. Caripense
(tzimbalo)
S. grandiflorum
(Potato tree)
S. melongena
(aubergine)
S. ovigerum
(easter eggplant)
S. taeniotrichum
6
Materials:
Solanum
as plant study system
Slide7Background: Trichome Morphology
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Slide8Questions1. How does trichome morphology vary across
Solanum genus?2. Are these differences limited to major
trichome types?
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Slide9ExperimentsMorphology and nomenclature of
trichomes
2. Density of trichomes
3. Dimensions of
trichome
subunits
Photo credits: Jesus Chavana
9
Slide10Result 1: Trichome Morphology: Terminology
10
Terminology
Definition
Attenuate
Base
Basilatus
Bifurcated
Brevicolate
Long and gradually tapering
Lowermost
part of the
trichomes
Emerging from a broad base
Divided into two branches
Short-necked
Slide11Result 1: Trichome Morphology: Primary Types
Glandular trichomes
Stellate non-glandular trichomes
Simple non-glandular trichome
Image credits: Sakshi Watts
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Slide12Result 1: Trichome Morphology: Major Types
With short central ray
Bifurcated
Porrect-geminate
With long central ray
Multitangulate
Image credits: Sakshi Watts
12
Slide13A
B
C
D
H
K
L
M
N
O
P
Q
R
S
T
G
I
J
E
F
Image credits: Sakshi Watts
13
Slide14Result 1: Trichome morphology and nomenclature
14
Species
Trichome
types (Major shape)
Additional features
(Simple smooth
uniseriate
-S;
Compound smooth
uniseriate- C; Simple pustulated uniseriate- P)
Line art of morphology
Ethiopian eggplant (
Solanum
aethiopicum
)
subulate
basilatus
non-glandular
trichome with distinct subsidiary cells
S
Slide15Generalized Regression; p≤ 0.0001
Result 2: Total trichome density vs Species
Image credits: Sakshi Watts
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Slide16Result 2: Total glandular trichome vs Species
Image credits: Sakshi Watts
16
Generalized Regression; p≤ 0.0001
Slide17Result 2: Total non-glandular trichome density vs Species
Image credits: Sakshi Watts
17
Generalized Regression; p≤ 0.0001
Slide18Result 2: Trichome density
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Species
Trichome
types (Major shape)
Additional features
(Simple smooth
uniseriate
-S;
Compound smooth
uniseriate- C; Simple pustulated uniseriate
- P)
Line art of morphology
Density
(
Average±Standard
error;
trichome
number/mm
2
leaf area)
Ethiopian eggplant (
Solanum
aethiopicum
)
subulate
basilatus
non-glandular
trichome
with distinct subsidiary cells
S
4.73±1.02
Slide19Result 3: Trichome dimensions
Dimensions of trichomes (in µm) present on abaxial leaf surface of
Solanum grandiflorum
1
2
3
4
5
6
7
8
9
10
11
12
Image credits: Sakshi Watts
19
Slide20Result 3:
Trichome
dimensions
20
Species
Trichome
types (Major shape)
Additional features
(Simple smooth
uniseriate
-S;
Compound smooth
uniseriate
- C;
Simple
pustulated
uniseriate
- P)
Line art of morphology
Density
(
Average±Standard
error;
trichome
number/mm
2
leaf area)
Dimensions
(in µm; Length in case of non-glandular
trichome
; Length*width of gland in case of glandular
trichome
;
Average±Standard
error)
Ethiopian eggplant (
Solanum
aethiopicum)
subulate basilatus non-glandular trichome with distinct subsidiary cells
S4.73±1.02
153.71±15.67
Slide21ConclusionsVariety of trichomes are present in
Solanum genus with stellate non-glandular, simple non-glandular and glandular trichomes being the primary types.
The morphological differences of trichomes are not only limited to species but are present within each species and leaf surface as well. This study can act as a referral source for further studies of trichomes
and their relationships with biotic and abiotic.
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Slide2222
Slide23Acknowledgments
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