vessels 2020 02 27 Semmelweis University Budapest Dr Nandor Nagy Developing blood vessels in zebrafish embryo RED neurons GREEN blood vessels 5 dpf TgfliEGFP ID: 935598
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Slide1
Histology of the blood vessels2020 02 27
Semmelweis University, Budapest
Dr. Nandor Nagy
Slide2Developing blood vessels
in zebrafish embryo
RED:
neurons
GREEN:
blood vessels
5 dpf Tg(fli-EGFP)
Nagy N et al, 2009 Developmental Biology
Slide3Slide4:Pericytes are fibroblast-like cells with extensive cytoplasmic processes that wrap around endothelial cells.
Slide5Histology of vessels1. Elastic arteries 2. Muscular arteries3. Arterioles4. Capillaries5. Venules6. VeinsLayers of the wall:Tunica intima (endothelium)Tunica media (smooth muscle / elastic fibers)Tunica adventitia (loose connective tissue)
Slide6Slide7Elastic artery
Slide8Elastic artery (HE)T. intima: endothelium, subendothelial layerT. media: 40-70 fenestrated elastic membrane, smooth muscle cell layers in betweenT. adventitia: connective tissue, vasa vasorum
Slide9Elastic arteryElastic fibers„Roller blind”
Slide10vasa vasorum
Slide11Internal elastic membraneExternal elastic membraneT.intimaMuscular artery (orcein
staining; dyes extracted from several species of lichen)
Slide12http://img3.wikia.nocookie.net/__cb20140529213818/vroniplag/de/images/3/35/Pew_02_diss.pngElsevier Kierszenbaum:Histology and Cell Biology: An introduction to pathologyMuscular artery
Slide13membr.elastica int.
Small artery
Slide14Arteriolehttp://micro.ronaldschulte.nl/esophagus/05_schema_arteriole.JPGhttp://www.edu.upmc.fr/histologie/A/arteriole/pages/ariole01.htm
Slide15Microcirculation1 Precapillary arteriole (sphincter)(endothelium, lamina basalis, 1 layer of smooth muscle)2 Capillary3 Postcapillary venule (endothelium, lamina basalis, pericyte)
Slide16Capillaries (toluidine blue)wall: only endothelium and lamina basalis(pericytes often surround the capillaries)
Slide17https://quizlet.com/32301631/histology-5-blood-circulatory-system-and-hemopoiesis-flash-cards/
Slide18Slide19Periferal vessels: vein valves, vessels of the limbs
Slide20VenulesElsevier, Ovalle & Nahirney: Netter’s Essential Histology
Slide21Small and middle sized veinshttp://histohelp.files.wordpress.com/2011/09/screen-capture2.png
Slide22Large veinT. intima1. endothelium2. subendothelial layerT. media3. smooth muscle and connective tissueT. adventitia4. longitudinal smooth muscle bundles, connective tissue5. collagen fibres
Slide23Most vein are equiped with one-way valves to ptevent blood flowing in reverse direction!
Slide24Lymph vesselsArterioleVenuleLymph vessel
Slide25vessel typeTunica intimaTunica mediaTunica adventitia Internal elastic membrane (IEM)elastic arteryendothel, subendothel(IEM)fenestrated elastic lamellae / membranessmooth musclefibrocytes, macrophages, elastic and collagen fibers, vasa vasorum, nervesmuscular artery
endothel, subendothelIEMsmooth muscle (spiral) few elastic fibers, circular collagen
fibers (ext. elastic membr.)fibrocytes, collagen and elastic fibers, vasa vasorum, nervessmall arteryendothel, subendothelIEM5-10 layers of
smooth
muscle, collagen fibers
thinner than the tunica media
arteriole, precapillary arteriole
endothel
(IEM)
2-5 layers of smooth muscle
precap.art.: 1 layer smooth muscle
pericyte, some collagen fibers
capillary
endothel (continuous, fenetrated or discontinuous)
pericytepostcapillary venule
endothel pericytevenuleendothel
some smooth muscle cellssome collagen, elastic and reticular fiberssmall veinendothel 2-3 layers of smooth muscle
loose conn. tiss., thicker than the media, longitudinal smooth muscle, collagen and elastic fibers, middle sized veinendothel, subendothel(IEM)circular smooth muscle layers
loose conn. tiss., thicker than the media, longitudinal smooth muscle, collagen and elastic fibers,
large vein
endothel, subendothel
(IEM)
smooth
muscle, varying thickness, collagen fibers
loose
conn
.
tiss
.,
thicker
than
the
media
,
longitudinal
smooth
muscle
,
collagen
and
elastic
fibers
, vasa
vasorum
,
nerves
,
lymph
vessels