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Flow Cytometry/ Bone Marrow Laboratory Flow Cytometry/ Bone Marrow Laboratory

Flow Cytometry/ Bone Marrow Laboratory - PowerPoint Presentation

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Uploaded On 2022-05-31

Flow Cytometry/ Bone Marrow Laboratory - PPT Presentation

Department of Pathology and Laboratory Medicine Located 23655 Hours of Operation 8am6pm MF 8am5pm Saturday Follow a Bone Marrow from collection to diagnosis Laboratory Testing ID: 912653

marrow bone cytometry flow bone marrow flow cytometry laboratory cell technologists leukemia lymphoma testing cells lab stem medical transplant

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Slide1

Flow Cytometry/ Bone Marrow Laboratory

Department of Pathology and Laboratory Medicine

Located 2-3655

Hours of Operation:

8am-6pm M-F 8am-5pm Saturday

Follow a

Bone

Marrow from collection to diagnosis

Laboratory

Testing

Total tests

T&B

cell Lymphocyte Subset

4000

CD34 Stem Cell

525

Bone Marrow

biopsies

1250

Lymphoma/Leukemia

Workups (Flow Cytometry)2650Consult Cases 800

Flow Cytometry and Bone Marrow Workload January-December 2015

The staff of the Bone Marrow lab assist in most of the Bone Marrow biopsies performed at UR Medicine. The technologists travel to the site of the procedure and bring the majority of the supplies needed. They help ensure that a quality specimen has been collected and make slides at the bedside.

After collection, the technologists return to the laboratory where they accession and process sample for review by the Hematopathologist.

The Hematopathologist , using slides and patient history orders appropriate flow cytometry testing on Bone Marrow samples. .

The Medical technologists manually stain all flow cytometry samples. Flow Cytometry panels are created to help screen for a variety of hematopoietic diseases.

The red and blue lasers excite stained cells and direct them to the appropriate detector….

….of a flow cytometer. The Flow cytometer uses cell size and complexity to divide cells into different populations. The fluorescent stains allow for the identification of Lymphoma, Leukemia and other diseases.

The SPA (sample prep assistant) enables

walkaway

sample preparation of LMH3 samples for flow cytometry.

T&B

cell

subset testing

(LMH3

) aids in the treatment of HIV and post solid organ transplant patients.

The Flow Cytometry/Bone Marrow lab consists of an integrated team of 4 Hematopathologists,1

Supervisor

, 2 Medical Technologist Specialists, 5 Medical Technologists, 1 Support Tech, and a rotation of Hematopathology Fellows and Pathology Residents. The Laboratory is highly interactive with the Wilmot Cancer Center, Adult and Pediatric Bone Marrow Transplant Services and Pediatric Hematology and Oncology.

In addition to Bone Marrow, the laboratory also processes lymph nodes, spleen

, blood,

body fluids, solid tumor cells, fine needle aspirates and CSF. They also aid the Stem Cell Lab in work up of Hematopoietic Stem Cell collections from Bone Marrow Donors for Transplant .

SPA

CLL

(Chronic Lymphocytic

Lymphoma/Leukemia)

AML

(Acute Myelogenous Leukemia)

Top Row (L-R

) Dr. Andrew Evans, Judy Miller, Dr. Richard Burack, Brian Smith, Roberta Thayer, Melissa Martin, Dr. Chad Hudson.

Bottom Row (L-R

) Nancy Halstead, Libby Miller, Sue Polochock, Pat Leary.

Not pictured: Dr. Arch Perkins, Dr. John Bennett, and Sue Martin