Laura Kasper 1 Abbas Zein AlDin 1 Rudolf Volkmer 2 Jürgen Bruns 1 and Klaus Petermann 1 1 Technische Universität Berlin FG Hochfrequenztechnik Photonik Einsteinufer 25 Berlin 10587 Germany ID: 1036529
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1. Fix-Wavelength Multi-Analyte Detection with serial SOI Ring ResonatorsLaura Kasper1, Abbas Zein Al-Din1, Rudolf Volkmer2, Jürgen Bruns1, and Klaus Petermann11Technische Universität Berlin, FG Hochfrequenztechnik - Photonik, Einsteinufer 25, Berlin, 10587, Germany2Institut für Medizinische Immunologie, Charité - Universitätsmedizin, Lindenberger Weg 80, 13125 Berlin, Germany
2. OverviewIntroduction Motivation Ring Resonator BasicsThermal Modulation for Fix-Wavelength MeasurementFix-Wavelength protein interaction measurementExperimental setup Sensor array preparationExperimental procedure and ResultsSummarySeite 2 Fix-Wavelength Multi-Analyte Detection with serial Silicon-On-Insulator Ring ResonatorsL. Kasper | I3S2021
3. 1. IntroductionMotivation for SOI Ring Resonators:Working label-free: No fluorescent or enzymatic label needed No incubation timeOptical Ring Resonators on silicon: Small sensor footprintLow cost fabricationMicroelectronic integrationSOI Ring Resonators as biosensors:Very low limit of detectionSpecifictiy by surface functionalizationMultisample-tests Seite 3 Fix-Wavelength Multi-Analyte Detection with serial Silicon-On-Insulator Ring ResonatorsL. Kasper | I3S2021
4. Seite 4 Basic working PrincipleLight - sample interaction via evanescence field Molecule binding results in an effective refractive index change and thus in a wavelength shiftChemical functionalization of the ring waveguide for antigen-selective surface Opens the door to complex media analyzation Antibody 1Antibody 1Antibody 2Problems: no sensor assignment in serial arrangementexpensive tunable laser sourceslow scanning repetition rate 1. IntroductionFix-Wavelength Multi-Analyte Detection with serial Silicon-On-Insulator Ring ResonatorsL. Kasper | I3S2021Antibody 2Transmission (dBm)
5. Seite 5 Fix-wavelength Measurements by thermo-optical modulation: Standard optical Measurement: sweep of input wavelengthFix-Wavelength: Using the thermo-optical effect of silicon Application of an AC Voltage to a heating electrode for thermo-optical modulation 1. IntroductionFix-Wavelength Multi-Analyte Detection with serial Silicon-On-Insulator Ring ResonatorsL. Kasper | I3S2021λres = , m Ν nres = , m Ν
6. Measurement Set-up:Seite 6Fix-Wavelength Multi-Analyte Detection with serial Silicon-On-Insulator Ring ResonatorsL. Kasper | I3S20212. Fix-wavelength protein interaction measurementSignal gen.inoutR1 (Ref)R2R3R4R5t
7. Seite 7 Sensor Preparation:Initial surface silanization (immersion, 2% APTES in toluene for 1h)Immobilization of two different antibodies (biotin, hexa-His-complex)3mg/ml biotin in DI-Water for 1h250µl/ml ac-hexa-His-peptide in EDC and OSu, 3 x 15 min 2. Fix-wavelength protein interaction measurementFix-Wavelength Multi-Analyte Detection with serial Silicon-On-Insulator Ring ResonatorsL. Kasper | I3S2021Biotinac-hexa-His-peptideinoutR1 (Ref)R2R3R4R5
8. Experiment:specific antigen lead to the sensor array via microfluidic systemDetecting and tracking the response of each ring separatetely over timeParameters:f = 3 Hz / RingSignal = Square-Root shaped voltage, periodicAnalyte via Microfluidics - Steps: 2 min PBS - 2 min Streptavidin (Biotin antagonist) Seite 7 2. Fast Protein interaction measurement - Experiment Fix-Wavelength Multi-Analyte Detection with serial Silicon-On-Insulator Ring ResonatorsL. Kasper | I3S2021
9. 4. SummarySeite 9 Fix-Wavelength Multi-Analyte Detection with serial Silicon-On-Insulator Ring ResonatorsL. Kasper | I3S2021Measurement of multiple biosensors simultaneously strongly desired for multi-analyte testsUsing thermo-optical modulation in a time-division-multiplex schemeenables the possibility to use simple ring resonator structures (all-pass-configuration) for multi-analyte tests for e.g. in Lab-on-Chip devicesno expensive tunable laser requiredcompact set-up
10. 4. SummarySpecial thanks to:Seite 10 Technical assistance: from M. Lucht, B. Bujok, M. Mahnkopf, M. Gruse, I. Kretzschmar and E. BroseFix-Wavelength Multi-Analyte Detection with serial Silicon-On-Insulator Ring ResonatorsL. Kasper | I3S2021
11. Thank you for your attentionSeite 11Fix-Wavelength Multi-Analyte Detection with serial Silicon-On-Insulator Ring ResonatorsL. Kasper | I3S2021
12. 12Fix-Wavelength Multi-Analyte Detection with serial Silicon-On-Insulator Ring ResonatorsL. Kasper | I3S2021
13. Seite 6 2. SetupFix-Wavelength Multi-Analyte Detection with serial Silicon-On-Insulator Ring ResonatorsL. Kasper | I3S2021
14. Optical inline measurements – Microfluidic systemSeite 14Optimized control over fluid volumesPredefined and continous flow speedsDefinition of speed and volume for each protocol stepMicrofluidic System (LabSMITH)Fix-Wavelength Multi-Analyte Detection with serial Silicon-On-Insulator Ring ResonatorsL. Kasper | I3S2021
15. 3. Results - IR Surface CharacterizationCharacterization on Si with IR-spectroscopic ellipsometry:900 – 1200 cm-1 relevant range for investigations on Si/SiO2 surfaceSi-O-Si band (1045 cm-1) and Si-O-C (1111 cm-1) decreasing with increased concentrationIndicates lower APTES densitiy at the native oxide of the Si-surface for increased concentrationsSeite 1510451111Fix-Wavelength Multi-Analyte Detection with serial Silicon-On-Insulator Ring ResonatorsL. Kasper | I3S2021