CTL MIII MIII MIII MIII MIII 1 2 3 4 5 6 7 8 9 10 Stain free loading normalization Red Outline 120kDa Thrombomoduline Red Outline 75kDa Stain free loading ID: 919322
Download Presentation The PPT/PDF document "HIF1 alpha CTL CTL CTL CTL" is the property of its rightful owner. Permission is granted to download and print the materials on this web site for personal, non-commercial use only, and to display it on your personal computer provided you do not modify the materials and that you retain all copyright notices contained in the materials. By downloading content from our website, you accept the terms of this agreement.
Slide1
HIF1 alpha
CTL
CTL
CTL
CTL
CTL
MIII
MIII
MIII
MIIIMIII12345678910
Stain free loading normalization
Red Outline: 120kDa
Thrombomoduline
Red
Outline: 75kDa
Stain free loading normalization
CTLCTLCTLCTLCTLMIIIMIIIMIIIMIIIMIII12345678910
Bcl2
CTL
CTL
CTL
CTLCTLMIIIMIIIMIIIMIIIMIII12345678910
Stain free loading normalization
Red Outline: 26kDa
Bax
CTL
CTL
CTL
CTL
CTLMIIIMIIIMIIIMIIIMIII12345678910
Stain free loading normalization
Red
Outline
: 21kDa
Slide2Tissue Factor
CTL
CTL
CTL
CTL
CTL
MIII
MIII
MIII
MIIIMIII12345678910Stain free loading normalization
Red Outline: 50kDa
Phospho Tissue Factor
CTL
CTL
CTL
CTL
CTL
MIII
MIIIMIIIMIIIMIII12345678910Stain free loading normalizationeNOS
Red
Outline
: 50kDa
CTLCTLCTLCTLCTLMIIIMIIIMIIIMIIIMIII12
3
4
5
6
7
8
9
10Stain free loading normalizationRed Outline: 137kDaP eNOSCTLCTLCTLCTLCTL
MIII
MIII
MIII
MIII
MIII
1
2
3
4
5
6
7
8
9
10
Stain
free
loading
normalization
Slide3VCAM
CTL
CTL
CTL
CTL
CTL
MIII
MIII
MIII
MIIIMIII12345678910Stain free loading normalization
Red Outline: 50kDa
ICAM
Red
Outline
: 100kDa
CTL
CTLCTLCTLCTLMIIIMIIIMIIIMIIIMIII12345678910Stain free loading normalizationEPO mw 30-34kDa coupé 50kDaRed Outline: 35kDaCTLCTLCTLCTL
CTLMIII
MIII
MIII
MIII
MIII12345678910Stain free loading normalizationProtein quantification Western Blotting was carried out according to standard protocols using specific antibodies against: HIF-1α, EPO (BD Transduction-Laboratories, Pont de Claix, france), eNOS total and phophorylated
Ser1177, Bax and Bcl2 (Cell-Signaling, Saint Quentin-Yvelines, France),
Thrombomodulin, total and
phophorylated Tissue-Factor (
Abcam
, Cambridge, United-Kingdom), and InterCellular
Adhesion Molecule-1 (ICAM-1) and VCAM-1 (Bioss
, Woburn, Massachusetts, USA), E-Selectin (
Sigma-Aldrich, Lyon, France). Top: Stain Free gel for normalization, Middle: full image of the western blot, Bottom: quantification of the indicated (red square) bands, normalized to the stain free gel.