Dan Schwarz 91917 Why do we care about aseptic technique Why do we care about aseptic technique Control Why do we care about aseptic technique Control Control Why do we care about aseptic technique ID: 1006696
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1. Aseptic Technique for Yeast CultureDan Schwarz9.19.17
2. Why do we care about aseptic technique?
3. Why do we care about aseptic technique?Control
4. Why do we care about aseptic technique?ControlControl
5. Why do we care about aseptic technique?ControlControlControl
6. Why do we care about aseptic technique?ControlBacteria/other fungus is a major competitive pressureControlControl
7. Why do we care about aseptic technique?ControlBacteria/other fungus is a major competitive pressureControlSporadic contamination is not reproducible or specificControl
8. Why do we care about aseptic technique?ControlBacteria/other fungus is a major competitive pressureControlSporadic contamination is not reproducible or specificControlCompetitive pressure from a contaminant will confound your experiment
9. So what do we do?Spray down working surfaces with 70% EtOH
10. So what do we do?Spray down working surfaces with 70% EtOHUse sterile glassware, filter pipette tips, media, buffers, etc.http://www.uvm.edu/safety/sites/default/files/autoclaveprocess.jpg
11. So what do we do?Spray down working surfaces with 70% EtOHUse sterile glassware, filter pipette tips, media, buffers, etc.Keep things capped AS MUCH AS POSSIBLE
12. So what do we do?Spray down working surfaces with 70% EtOHUse sterile glassware, filter pipette tips, media, buffers, etc.Keep things capped AS MUCH AS POSSIBLEAvoid loose clothing/hair
13. So what do we do?Spray down working surfaces with 70% EtOHUse sterile glassware, filter pipette tips, media, buffers, etc.Keep things capped AS MUCH AS POSSIBLEAvoid loose clothing/hairDon’t touch your laptop/bag/food/water with your gloves
14. So what do we do?Spray down working surfaces with 70% EtOHUse sterile glassware, filter pipette tips, media, buffers, etc.Keep things capped AS MUCH AS POSSIBLEAvoid loose clothing/hairDon’t touch your laptop/bag/food/water with your glovesBe tidy!
15. Any questions?