Limited Product WarrantyLife Technologies Corporation and/or its afli - PDF document

Limited Product WarrantyLife Technologies Corporation and/or its afliate(s) warrant their products as set forth in the Life Technologies’ General Terms and Conditions of Sale found on Life Technologies’ website at www.lifetechnologies.com/termsandconditionshave any questions, please contact Life Technologies at www.lifetechnologies.com/supportImportant Licensing Information These products may be covered by one or more Limited Use Label Licenses. By use of these products, you accept the terms LIFE TECHNOLOGIES CORPORATION AND/OR ITS AFFILIATE(S) DISCLAIM ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, OR NON-INFRINGEMENT. TO THE EXTENT ALLOWED BY LAW, IN NO EVENT SHALL LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) BE LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE USE THEREOF.© 2013 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation and/or its afliate(s) or their respective CLOSE Print ProtocolPrint Pop-Ups CLOSE Limited Product WarrantyLife Technologies Corporation and/or its afliate(s) warrant their products as set forth in the Life Technologies’ General Terms and Conditions of Sale found on Life Technologies’ www.lifetechnologies.Technologies at www.lifetechnologies.Important Licensing Information These products may be covered by one or more Limited Use Label Licenses. By use of these products, you accept the LIFE TECHNOLOGIES CORPORATION AND/OR ITS AFFILIATE(S) DISCLAIM ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED NOT LIMITED TO THOSE OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, OR NON-INFRINGEMENT. TO THE EXTENT ALLOWED BY LAW, IN NO EVENT SHALL LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) BE LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE USE THEREOF.© 2013 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies their respective owners. CLOSE Choosing the Right Gel Type for Your Application Life Technologies Protein GelsGel % availableSeparation rangeShelf lifeAverage run ApplicationsBolt™ Bis-Tris Plusproteins. Neutral pH environment minimizes protein modi cations. Wedge well design can accommodate large sample volumes. Ideal for Western blot transfer and analysis, and all techniques in which protein integrity is crucial.NuPAGE Bis-TrisSeparation of small- to medium-sized proteins. Neutral pH environment minimizes protein modi cations.NuPAGE Tris-AcetateSeparation of larger proteins.TricineSeparation of small proteins.NativePAGE™ Bis-TrisSeparation of native proteins.Tris-GlycineSeparation of small- to medium-sized proteins using E-PAGE™E-PAGE™ E-PAGE™ High-throughput for recombinant production analysis and protein pro ling.Separation of proteases based on size and activity.ZymogramIdentifying active proteases that utilize casein or CLOSE Choosing a Well Format and Gel Thickness Sensitivity of DetectionAmount of Puried Protein per WellAmount of Cell Lysate per WellWestern: Chemiluminescent, Chromogenic, and FluorescentThe following is a comprehensive list of gel well formats. Not every format is available for every gel type. Recommended loading volumes for best results1 wellIPG well2D well5 well9 well10 well12 well15 well17 well 1 well IPG well 2D well 5 well 9 well 10 well 12 well 15 well 17 well recommended resolution*Per protein band, * NuPAGE gels have a higher protein load capacity than Tris-glycine or Tricine gels. CLOSE Print Options Choosing a Well Format and Gel Thickness Sensitivity of DetectionAmount of Puried Protein per WellAmount of Cell Lysate per WellWestern: Chemiluminescent, Chromogenic, and FluorescentThe following is a comprehensive list of gel well formats. Not every format is available for every gel type. Recommended loading volumes for best results1 wellIPG well2D well5 well9 well10 well12 well15 well17 well 1 well IPG well 2D well 5 well 9 well 10 well 12 well 15 well 17 well recommended resolution*Per protein band, * NuPAGE gels have a higher protein load capacity than Tris-glycine or Tricine gels. CLOSE Choosing the Right Gel Type for Your Application Life Technologies Protein GelsGel % availableSeparation rangeShelf lifeAverage run ApplicationsBolt™ Bis-Tris Plusproteins. Neutral pH environment minimizes protein modi cations. Wedge well design can accommodate large sample volumes. Ideal for Western blot transfer and analysis, and all techniques in which protein integrity is crucial.NuPAGE Bis-TrisSeparation of small- to medium-sized proteins. Neutral pH environment minimizes protein modi cations.NuPAGE Tris-AcetateSeparation of larger proteins.TricineSeparation of small proteins.NativePAGE™ Bis-TrisSeparation of native proteins.Tris-GlycineSeparation of small- to medium-sized proteins using E-PAGE™E-PAGE™ E-PAGE™ High-throughput for recombinant production analysis and protein pro ling.Separation of proteases based on size and activity.ZymogramIdentifying active proteases that utilize casein or CLOSE Choosing the Right Gel Percentage and Buffer NativePAGE™ gels3–12%Novex® Tris-glycine gels NuPAGE® Tris-acetate gels4–16 %3 –8%6%4–12%8–16%4–20%7%0% 100% 1,048 kDa 720 kD 480 kD 242 kD 146 kD 1,048 kD 720 kDa 480 kDa 242 kDa 146 kDa 66 kDa 20 kDa 1,048 kDa 720 kD 480 kD 242 kD 1,048 kDa 720 kDa 480 kDa 242 kDa 146 kDa 1,048 kD 1,048 kD 1,048 kDa 720 kD 10 % 1,236 kD 720 kDa 720 kD 20 % 480 kD 1,048 kD 480 kD 242 kD 30 % 480 kDa 242 kD 146 kD 720 kDa 40 % 242 kDa 146 kD 50 % 66 kD 146 kDa 60 % 66 kD 242 kDa 66 kDa 70 % 20 kD 146 kDa 80 % 66 kDa 20 kDa 20 kD 90 % 480 kDa Migration of Mark12™ Unstained Standard under denaturing conditionsMigration patterns of NativeMark™ Unstained Native Protein Standard on CLOSE