XI Adjust the pH of the enzyme buffer at the temperature at which it will be used Manycommon buffers are exceptionally sensitive to temperature The pH of a solution containing the buffer Tris dec ID: 953314
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BASIC RULES FOR HANDLING AN ENZYMEdated October 2011For the novicebasic hints to guide you through your first enzymatic reaction. For the experta refresher course and an aid for training your students. XI. Adjust the pH of the enzyme buffer at the temperature at which it will be used. Manycommon buffers are exceptionally sensitive to temperature. The pH of a solution containing the buffer Tris decreases 0.3 pH units for every 10C rise in temperature. More details on this phenomenon can be found in the Lab Hints section of BM Biochemical Vol. 1, No. 3, August XII. Detailed information is a
vailable on many enzymes. The most complete references are: Methods in Enzymology, published by Academic Press, Editorschief: Sidney P. Colowick and Nathan O. Kaplan. There are morethan 110 volumes in this series, covering an extensive range of topics. The Enzymes, 3rd edition, edited by Paul D. Boyer, an excellent, broad series with less emphasis on methodology than Methods in Enzymology. Methods of Enzymatic Analysis, 2nd and 3rd editions, Editorchief: Hans U. Bergmeyer, published by Verlag Chemie. Indepth discussion of techniques of analysis which use enzymes or which assay enzymes.