BOLEY NUTRACEUTICALS LTD. - PowerPoint Presentation

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BOLEY NUTRACEUTICALS LTD. DISTRIBUTORS OF TOTALLY DERMA

“Nutraceuticals:

The Future of Age Management?”

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What happens with ageing ?Reduction in hyaluronic acid, collagen and elastin fibres

Atrophy and laxity of the skin and mucous membranes

Painful and stiff joints

Hormonal imbalance Vaginal dryness DyspareuniaUrinary symptomsPoor body imageLow self-esteem

IAAGSW London 27-29 October 2017

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Can Oral Supplements Really Address Aesthetic and Physiological Ageing Processes?

Is it possible to increase collagen and hyaluronic acid internally through nutraceuticals?

Nutraceuticals: The Future of Age Management?

IAAGSW London 27-29 October 2017

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NUTRACEUTICALSTerm coined in 1989 by Stephen L. DeFelice, founder and chairman of the Foundation of Innovation MedicineA foodstuff (as a fortified food or dietary supplement) that provides health benefits in addition to its basic nutritional value

Global

Nutraceutical

Market: 259 Billion in 2012Projected to be $ 424 Billion in 2017ENA (European Nutriceutical Association)

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Nutraceuticals: COLLAGEN DRINKS

Claim to boost the body’s natural collagen production.

Marine collagen based

? Of absorption since nutritional supplement

? If absorbed, are the amino acids utilized for boosting collagen production in the body or for general energy production

Clinical studies show a particular

Hydrolysed

BOVINE Collagen branded ARTHRED® does absorb through the small intestine and does stimulate collagen production in the body.

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ARTHRED® Patented Hydrolysed bovine collagen 2-3 kilodaltons (

kDa

)

vs. 70 kDa for collagen / gelatine products. Digestible, highly absorbable, hypoallergenic Contains amino acids: glycine, proline, hydroxylysine, lysine and hydroxyproline. Comprise 50% of the substance by weight.Bovine Hydrolysed Collagen peptides resemble human collagen more closely than marine collagen. Gelatine origination by

Gelita

AG

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Bioavailability of ARTHRED®Hydrolysed

means

‘pre-digested’,

a MINUTE molecular size that reaches the small intestine undamaged by digestive enzymes and is optimally absorbed there.The benefits of an enzymatic hydrolysis are smaller short chain amino acid peptides which are uniform and consistent in size thereby guaranteeing a 95% absorption rate.Arthred’s guaranteed small short chain amino acid content, takes the burden off the digestive system. The product is already in the form needed for straight absorption through the small intestine – therefore the stomach does not need to break it down. This is particularly useful in clients who have compromised digestive systems as the protein in Arthred® does not need to be broken down by the digestive system.

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Bioavailability of ARTHRED®Superior absorption and bioavailability due to low molecular weight.

Greiling

et al. 1998, showed 10g

Arthred® significantly increased collagen amino acids in blood serum. Hydroxyproline rose in blood serum by 550% after Arthred® ingestion.

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Serum Amino Acid Concentration After Oral Administration Of ARTHRED®Hydroxyproline

Hydroxylysine

Glycine

Proline

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Skin Studies Using Bovine Hydrolysed CollagenProksch et al. (2014) conducted a double blind placebo controlled trial with 69 women aged 35-55 yrs old with Bovine hydrolysed collagen or placebo for 8 weeks.

The bovine hydrolysed collagen group showed significant improvement in skin elasticity, Skin moisture, and skin evaporation over placebo group.

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Skin Studies Using Bovine Hydrolysed Collagen (cont….)

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Hyaluronic AcidMajor component of dermis and cartilageMajor injectable filler for skinN-acetyl glucosamine + D-glucuronic acid=HA

Commonly found in nutritional supplements, moisturizers, and anti-aging creams

Problem: HA is not absorbed through skin or from GI tract due to high molecular weight

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Synovoderma: Hyaluronic AcidHighly absorbable, highly bioavailable form of oral HAVery Low molecular weight hyaluronic acid

50 -200kDa

(HA usually 1200-1500

kDA)An animal study with radiolabelled Synovoderma: Radiolabeled Hyaluronic Acid was detected in blood, muscles, salivary glands and bone within 30 mins of ingestion. Less than 5% of radioactivity appeared in faeces after 72 hours, showing a high absorption rate.

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New Collagen / HA / Nutraceutical

Collagen Drink Supplement

Introducing: Collagen/HA/nutrient Powder:

boosts collagen production, HA and hydration

addresses both the aesthetic (physical) signs of ageing and the underlying physiological processes.

Optimises not only for the skin but the entire body and its systems at the cellular level aiming to deliver visible improvements in overall skin, hair, nails,  joints, gut health, general well-being and gynaecological menopausal dryness.

once daily, pleasant tasting, easy to digest

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Collagen/HA Drink Supplement technology

The ingredients in

Collagen Drink Supplement®

are digested and absorbed in the small intestine and distributed via the blood vessels into the deeper layer of the dermis, where they multiply & stimulate fibroblast cells to produce more collagen and hyaluronic acid in the dermisCollagen Drink Supplement® is shown to deliver benefits not only for skin, but for joints, hair, nails, bone, gut inflammation, bladder, urethra, vagina and therefore is also a valuable supplement for peri-post menopausal women.

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Collagen/HA Drink Technology - COLLAGEN

10,000mg. of

Arthred

™, a proven

hydrolysed

bovine powdered collagen

pharmaceutical grade, patented, clinically proven ingredients

to support, maintain and promote cartilage tissue and collagen throughout the body

digestible, highly absorbable and well tolerated

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Bovine collagen is BEST FORM of collagen – identical to human3

Studies show

FOURFOLD

stimulation of fibroblasts, significant increase in collagen synthesis and decreased MMP2 activity4After 1 dose Arthred

™ Collagen – Hydroxyproline levels rise by

550%

(not possible through diet alone)

5

After 1 dose Collagen/HA drink® – Hydroxyproline levels rise between

1500% and 3500% as we will see later

*

Clinical evidence for therapeutic 10,000mg dose to boost collagen all over.

6

3. Cheryl

M.Burgess

. Cosmetic Dermatology p99-100 ISBN 3-540-23064-5 Springer

4. JOURNAL OF MEDICINAL FOOD J Med Food 14 (6) 2011, 618–624 Collagen Hydrolysate Intake Increases Skin Collagen Expression and Suppresses Matrix Metalloproteinase 2

ActivityVivian

Zague, Vanessa de Freitas, Marina da Costa Rosa, Georgia Alvares de Castro,

Ruy G. Jaeger, and Glaucia M. Machado-Santelli.5. Greiling, H and HW Stuhlsatz.  1998.  Personal Communication.6. Beuker, F, P Stehle

, M Ritsch, and H Theel.  1993. The influence of regular gelatin substitution at the amino acid levels in blood plasma.  

Ernährungs-Umschau. 40: 64.* Based on 12 week clinical skin trials in 2015 in US for Totally Derma formulation

Collagen/HA Drink Technology - COLLAGEN

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Collagen/HA Drink technology – HYALURONIC ACID

HA is not a component of most ‘collagen drinks’

Contains

Synovoderma

- Superior Patented

HA powder, 210g per serving

Molecular weight of just 50

kDaltons

, proven 95% absorption in studies

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Increased dermal HA leads to improved skin volume, optimal hydration, increased Collagen Synthesis, stimulation of fibroblasts, toxin elimination

Double blind studies show significant improvements in dryness, itchiness and redness for 100% of subjects over 6 weeks

9

8. Life Sciences Division, American Institute for Biosocial and Medical

Research.Absorption

, distribution and excretion of

labeled

hyaluronan after single oral doses in rats and beagle dogs. Alexander G Schauss,1 Lajos Balogh,2 Andras Polyak,2 Domokos Mathe,2

Reka

Kiraly,2 Gyozo Janoki29. Aesthetic Dermatology Vol. 12 109-120, 2002: Clinical Effects of dietary hyaluronic acid on dry, rough skin. Sato T, Sakamato O, Odanaka W, Yoshida K, and Urishibata

, O

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Collagen/HA Drink Composition:

Hydrolyzed Collagen 10,000 mg

Hyaluronic Acid 210 mg

Zinc 5mg

Vitamin C 250mg

Grape Seed Extract 160mg

Green Tea Extract 160mg

Alpha Lipoic Acid 60mg

Manganese 3mg

Copper 0.33mg

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USEFUL INGESTED ANTIOXIDANTS TO HELP ADDRESS AGEING TISSUEZinc

Co-factor needed for Collagen synthesis

procollagen, N-Proteinase & pro-collagen C-Proteinase

Green tea extractContains Catechins (polyphenol) A 12-week, double-blind, placebo controlled study of 60 women found that Green Tea Catechins were able to clinically improve skin elasticity, roughness, scaling, density, and water contentGrape seed extract Powerful antioxidant Contains phenols (proanthocyanidins) Protects cells

Alpha Lipoic Acid

is a water- and fat-soluble antioxidant that is capable of regenerating other antioxidants, such as vitamins C and E. It therefore exerts numerous anti-ageing effects in the body and skin.

Alpha lipoic acid research was done on human dermal fibroblasts in vitro (test tube) in cell-culture systems. The results showed that, when taken orally, Alpha Lipoic Acid helps to prevent cellular damage via its antioxidant properties. By protecting fibroblasts this helps to slow down the ageing process in the skin and stimulate repair.

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The Collagen/HA Drink® technology – Co-FactorsSeven different Co-Factors included to MAXIMISE

the effects of the Collagen and HA

synthesis and repair.

Ascorbic Acid (Vit C)CopperManganeseZinc Green Tea Extract

Grape Seed Extract

Alpha Lipoic Acid

All work synergistically.

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12 Week Evidence for Collagen/HA Drink SUPPLEMENT®Study Design and Subjects12-week usage study assessing the absorption of daily Nutraceutical Drink ingestionexamining absorption levels of the key ingredients, collagen and hyaluronic acid, in blood serum and skin biopsies.

Study Population: 6 women aged 46–65 years with mild to moderate general facial lines and coarse wrinkles in the eye area, skin dehydration and mottled pigmentation on the face.

Subjects continued their regular skin care regimen alongside consumption of the nutraceutical beverage.

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12 Week Evidence for Collagen/HA Drink SUPPLEMENT®AssessmentsBlood serum was assessed at baseline (fasting) and 2 hours after beverage consumption for levels of hydroxyproline

(as a marker for collagen serum absorption) and HA

Skin biopsies

from non-sun exposed skin (hip region) were collected at baseline and after 12 weeks of daily use of the beverage, and were sectioned and immunofloressence stained for hematoxylin and eosin (H&E, control) and for the following markers:Elastin as a marker for collagen absorptionColloidal iron as a marker for HA absorptionVisia Skin Analysis Machine facial assessment at baseline and 12 weeksRetrospective self-reporting questionnaire

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Results (1)

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Results (2) see explanation

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Results (3)Percentage increase in blood serum HP and HA, 2 hours after consuming the formulation contained in Nutraceutical Drink Supplement

Subject 1

Subject 2

Subject 3Subject 4

Subject 5

Subject 6

Increase in HP

+2250%

+1500%

+3500%

+2325%

+3150%

+2700%

Increase in HA

+47.5%

+173%

+83%

No

change

−23.2%−13.8%

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Results (4)Skin biopsy sections – elastin staining (marker for collagen absorption)

Baseline

12 weeks

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Results (5)Skin biopsy sections – colloidal iron staining (marker for HA absorption)

Baseline

12 weeks

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Visia Skin Study Results – 10 week daily consumptionAged 47 - Nasolabial Fold Baseline

Aged 47 - Nasolabial Fold 10 weeks

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Visia Skin Study Results – 10 week daily consumptionAged 52 - Crows Feet Baseline

Aged 52 - Crows Feet 10 weeks

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Skin Study ConclusionThese studies support the notion that ingesting specific ingredients can result in an anti-ageing and ‘health-boosting’ effect on the skin and body. The right balance of specific, optimal quality and high purity ingredients is crucial in order to ensure therapeutic levels are absorbed and are bioavailable for body utilisation.

The specific formulation of TOTALLY DERMA Nutraceutical Drink works from within to stimulate the body’s own production of collagen, elastin and HA, thus addressing the underlying physiological ageing process.

Optimisation not only for the skin but the entire body and its systems at the cellular level aiming to deliver visible improvements in overall skin, hair, nails,  joints, gut health, general well-being and gynaecological menopausal dryness.

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TestimonialsProf E. Briden - Dermatologist US “We are really excited by the results we are getting in our scientific study. The results include markedly elevated blood serum levels for Hydroxyproline post consumption, which shows the impressive absorption levels. The study group are happy with the improvements in their skin so far.”“The skin biopsies are truly remarkable! They show increased elastin and GAG's and a marked decrease in periocular wrinkles”.

“Two of the patients on the study commented that the product really helped their joints - they commented that their knees did not hurt when running and now they have been finished for a week, their knees hurt. “

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Testimonial from Consumer September 2017Totally Derma - totally over the moon...This is it, this is the one product that works - for me.No marine collagen (a must for me), just a clever combination of ingredients that when combined, work magic. 

First trial - wary of results, all too quickly my body responded and I felt 'good'. Within weeks my hair, nails and skin had benefited, even my bones and muscles felt stronger. I felt energised and in tune with my whole self, both physically and emotionally. Whatever was missing, my body found it in the product. I became a worshiper, never missing my daily fix and convinced that my improved appearance and improved energy was down to this pot of powder.

I could never explain how scientifically it works for me - it just does - I only hope it never goes away again in my lifetime.

Nikki M. Reigate, Surrey

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Totally Derma®I months supply = 1 tub

1 tub RRP = £105.00

Cost to patient = £3.50 /day