February 21 2022 MOPA Core Lab at UAB Located in Bevill Biomedical Research Building Specialized facilities equipment for performing MOPA Experienced MOPA technicians Scientific laboratory administrative support ID: 1038109
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1. David LaFon MDUAB Virtual Core DayFebruary 21, 2022MOPA Core Lab at UAB
2. Located in Bevill Biomedical Research Building Specialized facilities; equipment for performing MOPAExperienced MOPA techniciansScientific, laboratory, administrative supportNumerous previous/ongoing collaborations with UAB, US/international investigatorsThe MOPA Core Lab at UAB2Moon Nahm, MDDavid LaFon, MD
3. New/expanded vaccines (PCV71315/20)Clinical diagnosis of immune deficienciesStudy pneumococcal vaccinesMeasure immune functionWhy measure pneumococcal antibodies?Problem: Levels ≠ Function IgG antibody levels: Assays either unreliable or impracticalHajjar et al. 2018; JACILaFon & Nahm 2019; JACILee, Nahm et al. 2019; CVIStreptococcus pneumoniaeSørensen et al. 1988; Infect Immun
4. MOPAABCDPhagocytes(differentiatedHL-60 cells)Baby rabbit complementSerum (pneumococcal antibodies)4 serotypes of S. pneumoniae Dilution w/ 50% killingSerum dilutions1Opsonization index (OI)=SerotypesABCDSerum dilutionsLaFon, Nahm, Dransfield et al. [Abstract] ATS 2020 Virtual ConferenceMultiplexed opsonophagocytosis assay (MOPA)Measures pneumococcal antibody function via killing of bacteria by serum antibodiesInternationally accepted protocol (www.vaccine.uab.edu); Results standardized for interlaboratory comparison
5. Pneumococcal antibodies in COPDPCV7 vs PPV23 in COPDReduced antibody function is associated with COPD exacerbationsDiagnosis of primary immunodeficienciesPneumococcal IgG assays are unreliable for clinical diagnosisNovel approaches to analyzing MOPA data for clinical diagnosisPast projectsThe FutureMOPA Core LabDransfield, Nahm et al. 2012; Clin Infect DisLaFon & Nahm 2019; JACILaFon, Dransfield, Nahm et al. 2021; J Clin ImmunologyImmune defects in recurrent bacterial infectionsCOPDPrimary immune deficiencies Functional correlates for genomic & laboratory-based studiesEvaluate immune suppressing; immunomodulatory therapiesNovel diagnostic approaches to bacterial respiratory infections
6. Thank you!www.vaccine.uab.edudlafon@uabmc.edu