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NaPi Buffer NaPi Buffer

NaPi Buffer - PDF document

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Uploaded On 2021-08-10

NaPi Buffer - PPT Presentation

For research use only Advanced BioReagents Systems Hayward CA 94545 Tel 6504580155 Fax 5103720318 orderabioreagentscom wwwabioreagentscom 1 NaPi Buffer Cat C8063 readytouse buffer store ID: 861333

phosphate buffer napi procedures buffer phosphate procedures napi ready buffered saline 10x pbs protein dilution elisa gel laboratory page

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1 NaPi Buffer For r
NaPi Buffer For research use only ……………………………………………………………………………………………… ……………………………………………………………………………………………………………………………………………… ©Advanced BioReagents Systems • Hayward, CA 94545 Tel 650-458-0155 Fax 510-372-0318 • order@abioreagents.com • www.abioreagents.com - 1 - NaPi Buffer (Cat# C8063; ready-to-use buffer; store at RT) Introduction Sodium Phosphate is commonly used in many laboratory techniques. The buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures. It is often used for sample dilution in molecular biology and as protein diluents in Western blotting. Furthermore, the buffer can function as an equilibrator for gel filter columns ABS_BioTM ready-to-use NaPi Buffer is supplied as 10 mM Sodium Phosphate and 150 mM NaCl, pH 7.6 colorless buffers. Mg++, Ca++, DNase-, RNase- and Protease-Free. PBS does not contain any preservatives that may interfere with binding interactions. Kit Components NaPi Buffer: 500 mL Multiple size are available: 1000-10000 mL Storage and Handling: Store all of the components at room temperature. Shelf Life: 12 months after receipt. Features for All of PBS Buffers Formulated from analytical grade chemicals. Ideal for standardizing laboratory work. Ready to use in minutes. Applications Immunoassays. Immuno-histochemical procedures. Microbiological procedures. Tissue and cell culture procedures. Protein dilution. Protocol The buffer is filtered with 0.2 µm filter in sterile condition and it is ready to use. No pH adjustment is required. Related Products: TMB substrate solution(#C8010) ELISA Blocking Buffer(5x 25mL; #C8002) ELISA Washing Buffer(20x, 25mL; #C8003) Antibody Dilution Buffer(#C8004) Protein Loading Buffer(2x, 6x ; #C8005) SDS-PAGE Running Buffer(10x ; #C8006) Phosphate Buffered Saline (10x PBS, #C8007) Phosphate Buffered Saline with Tween 20(10x PBS-T; #C8008) Phosphate Buffered Saline(1xDPBS, #C8012) PAGE-gel transfer buffer(10x, #C8014) TE Buffer(#C8020) DNA Loading Buffer(#C8021) TBST Buffer(#C8024) TBST Blocking Buffer(#C8025 Phosphate Buffered Saline(1xPBS, #C8009) KPi Buffer (#C8063)