2930 April 2014 Malta COST Domain Biomedicine and Molecular Biosciences BMBS TD1002 European network on applications of Atomic Force Microscopy to NanoMedicine and Life Sciences Once more ID: 793903
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Slide1
Annual Progress Conference
29-30 April 2014, Malta
COST Domain - Biomedicine and Molecular Biosciences (BMBS)
TD-1002
European network on applications of Atomic Force Microscopy to
NanoMedicine
and Life Sciences
Slide2Once more,
everything
is here!
Slide3The main objective of the COST Action is to further develop and transfer the biophysical achievements of Atomic Force Microscopy to
NanoMedicine and clinical research.
Is AFM useful in a clinical environment?If yes, when, where, and how?
Slide4Evolution: In Krakow, on September 2012, MC decided to favor organizing specific Workshops under WG control.
Slide55
Imagerie
High Resolution Imaging
of Single Molecules
Dynamic Force Spectroscopy
(DFS)
Cellular Mechanics
Slide66
24 COST countries
AT, BE, CH, DE, DK, EL, ES, FI, FR, HR, HU, IE, IL, IT, LT, LU, NL, NO, PL, PT, RO, SRB,
TR, UK
Chair of the Action:
Prof. Pierre PAROT (FR)Vice Chair of the Action:Prof. Hans OBERLEITHNER (DE)DC Rapporteurs:
Prof
Pavle
ANDJUS
(RS)
DC Rapporteurs:
Prof
Spiros
H. ANASTASIADIS
(EL)
Science
officer
of the Action:
Dr Giuseppe LUGANO
Administrative officer of the Action:Ms Svetlana VOINOVA
Slide7Meetings 2010 Kickoff Meeting Brussels, December 10
(LO: Magdalena Radwanska)2011 WG Meeting Paris, May 10-13 (LO: Pierre
Parot)2011 MC Meeting August 23 // AFMBioMed Conference Paris 2011 (
LO: Simon Scheuring)
2012 WG
Meeting Linz February 3-4 // Linz Workshop 2012 (LO: Peter Hinterdorfer)2012 MC
Meeting Cracow September 14
(LO:
Magorzata
Lekka)
2012 Workshop Standardization Madrid, 18-19 October
(
LO: Ricardo
Garcia)
Workshop
NanoToxicology
Bucarest
, 12-13 November (LO: Marius Enachescu) BM0903 TD1206 2013 Workshop Standardization II Enschede, 21-22 March, (LO: Peter Schön)2013 Worshop Education Genoa Camogli
, 15-16 April (LO: Massimo Vassalli and Alessandro Podesta) Workshop Nanoparticules
Dijon, 3-4 July, (LO: Eric
Lewnieska)2013 MC & Workshop AFM for Drug Design Dubrovnik, 26-27 September (LO: Vesna
Svetlicic
& Zoran
Madic
)
2014
Workshop Standardization
Viterbo
, 20-22 March, (L.O. Salvatore Cannistraro)
2014
Workshop Standardization
Belvaux
, 1-3 July, (Jean-Nicolas Audinot)
join meeting with TD1204
Modelling Nanomaterial Toxicity (
MODENA
)
2014 MC & Workshop
Standardization
Cambridge, 223-25 September,
(L.O.
Robert Henderson)
2014 Final COST Action Meeting Paris, 28 November (L.O. Pierre Parot)
Schools
2011
Marcoule Summer
School,
August 30 – September 2; 26 trainees
(LO: Jean-Luc
Pellequer
)
2012 Cracow Summer
School
,
September 2012
(
LO:
Magorzata
Lekka)
2013
Marcoule
Summer School, 1-6 September 2013
(LO: Michael Odorico)
2014 Lille Summer School, 25-29 August
(L.O. Frank Lafont
)
Slide88From Croatia to France, Dr Galja
Pletikapić, Rudjer Bokovic; Institute, Zagreb(HR)
STSM Topic: Characterization of nanoparticles using AFM Reference From Italy to France, Prof. Anna Rita Bizzarri, University of Tuscia,01100 Viterbo(IT)STSM Topic: DFS standardisation
From France to Croatia, STSM Applicant:
Dr Christian Godon, CEA, Bagnols
sur Cèze STSM Topic: Standarization of nanoparticles
From France to Italy, STSM Applicant:
Mr
Jean-Marie TEULON,CEA,30200 BAGNOLS SUR CEZE
Cedex
(FR) ,
STSM Topic: DFS
standardisation
From France to Switzerland)
STSM Applicant:
Dr
Ludovic
Roussille,U1006 INSERM / Aix-Marseille Université, Marseille (FR) , STSM Topic: Combined AFM and TERS measurements of biological membranes From Russian Federation to Denmark
STSM Applicant:
Mr George Shevelev,Institute of Chemical Biology and Fundamental Medicine, Novosibirsk (RU) ,STSM Topic: AFM investigation of forse and thermodynamic parametras of native and mutanted double stranded DNAFrom Italy to United KingdomSTSM Applicant: Dr Massimiliano Papi,Università Cattolica del Sacro Cuore, Roma (IT) , STSM Topic: Development of the Atomic Force Microscopy for Ophthalmic Diagnosis
Slide99
STSM
Slide10Outputs from the Networking
Workshop Dijon July 2013
Standardization: Imaging single molecules and single particules; Force SpectroscopyWorkshop/MC Dubrovnik September 2013AFM for Drug Discovery / Standardization Mechanics and Education
Joint
PCMDDD-3 & COST Action TD 1002 sessionInvited Lectures Ratnesh Lal
, Srinivasan Ramachandran, Fernando Teran Arce, Preston Landon
,
University
of
California
San Diego, USA,
AFM
Nanoimaging
and
nanomechanics
for
nanomedicine
Robert Henderson,
University
of Cambridge, UK, G-quadruplex DNA and DNA origami: Insights from atomic force microscopy
Slide11TOPICS* High resolution Imaging (unknown
proteins)*
Cell Mechanics (gel, cells)* Force Spectroscopy
(antibody
antigene interaction)
* Nanoparticules (UP or sea water)
11
Added
value of
networking vs burning EU money!
Slide12The Dubrovnik procedure: how to standardize measuring
mechanical properties of
soft samples in AFM Jens Schäpe1, Tomas Luque
2, Holger Doschke
1, Hermann Schillers3, Mike Wälte
3, Juan José Uriarte2, Noelia Campillo2
,
Georgios
PA Michanetzis
4
,
Justyna
Gostek
5
,
Andra
Dumitru
6
, Elena T. Herruzo
6, Pierre Parot7, Massimiliano Galluzzi
8, Alessandro Podesta8, Simon Scheuring9, Yannis Missirlis4, Ricardo Garcia6, Michael Odorico
7, Malgorzata Lekka5, Felix Rico9, Jean-Luc Pellequer7, Hans Oberleithner3, Daniel Navajas2, Manfred Radmacher11 Institute of Biophysics, University Bremen, 28359 Bremen, Germany2 Institute for Bioengineering of Catalonia, University of Barcelona, and CIBER Enfermedades Respiratorias, 08028 Barcelona, Spain3 Institute of Physiology II, University of Münster, 48149 Münster, Germany
4 Department of Mechanical Engineering & Aeronautics, University of Patras, 265 04 Patras, Greece
5
The Henryk Niewodniczański Institute of Nuclear Physics, Polish Academy of Sciences, 31-342 Cracow, Poland6
Institute of Microelectronics Madrid, 28760
Tres
Cantos, Madrid, Spain
7
CEA
iBEB
, Department of Biochemistry and Nuclear Toxicology, F-,
30207
Bagnols-sur-Cèze
, France
8
CIMaINa
Dept
.
of
Physics
and
CIMAINA,
Università
degli
Studi di Milano,
Università degli Studi di Milano, via Celoria 16, 20133 20133 Milano, Italy 9 U1006 INSERM, Aix-Marseille University, Parc Scientifique et Technologies de Luminy, 13009 Marseille, Francesubmitted
12
Slide1313
Slide1414During Year 3 we had a very good collaboration with the company NanoAndMore.They help us a lot providing free AFM tips allowing standardization experiments in several team involved in the Action.
Slide15Slide1616
* Atomic force microscopy characterization of silver nanoparticles interactions with marine diatom cells and extracellular polymeric substance.
G. Pletikapić, V. Žutić, I. Vinković Vrček, V. Svetličić, J. Mol. Recognit., (2012), DOI: 10.1002/jmr.2177.
* Polymer Networks Produced by Marine Diatoms in the Northern Adriatic Sea.
V. Svetličić, V. Žutić, T. Mišić Radić, G. Pletikapić, A. Hozić Zimmermann, and R. Urbani,
Marine Drugs, 9
(2011) 666-679.
* Quantitative Nanomechanical Mapping of Marine Diatom in Seawater Using Peak Force Tapping AFM
G. Pletikapić, A. Berquand, T. Mišić Radić, and V. Svetličić,
Journal of Phycology
,
48
(2012) 174–185
.
* Adhesive-free colloidal probes for nanoscale force measurements: Production and characterization.
Indrieri, M.; Podesta, A.; Bongiorno, G.; Marchesi, D.; Milani, P., Review of Scientific Instruments 2011,
82
(2), 023708-11.
* Fluorescence and atomic force microscopy imaging of wall teichoic acids in Lactobacillus plantarum.
Andre, Deghorain, va Swam, Kleerebezem, Hols and Dufrêne, ACS Chem. Biol.,
6
(2011) 366-376.* Effect of Antimicrobial Peptide-Amide: Indolicin on Biological Membranes. Végh, Nagy, Balint, Kerényi, Rakhely, Varo and Szegletes, Journal of Biomedicine and Biotechnology, Vol 2011 (2011) ID 670589, 6 pages doi: 10.1155/2011/670589.* Forces guiding assembly of light-harvesting complex 2 in native membranes.Lu-Ning Liu, Katia Duquesne, Filipp Oesterhelt, James N. Sturgis, and Simon Scheuring
, PNAS, June 7, 2011, 108 (23) 9455–9459.
*
Two barriers for sodium in vascular endothelium?Oberleithner, Annals of Medicine (2012), DOI: 10.3109/07853890.2011.653397, Mini Review* Nanomechanics
and Sodium Permeability of Endothelial
Surface Layer Modulated by Hawthorn Extract WS 1442
.
W. Peters, V. Drueppel, K. Kusche-Vihrog, C. Schubert, H. Oberleithner, PloS ONE,
2012
,
7
(
1
)
e29972
.
Slide17Nanomechanical characterization of
eye l
ens cells and the effect of
cytoskeletal
drugs
WG1 and WG4
17
Cytoskeletal
d
rugs
:
no effect on
c
ell elasticity
Nanoparticles and the environment Ag NPs and marine diatoms
WG4
Ag NP aggregtion in SW
Ag NPs: diatom cell wall penetration
Slide1818Over
200 scientists participated to Action activities (female 28% , Early Stage Researcher 35
%) 6 workshops on Standardization (5), Education (1) 4 active groups of labs involved in 4 topics of AFM in biology: High resolution, Mechanobiology, Force Spectroscopy, Nanotoxicology
First
Summer School 2011: 26 trainees, 12 instructorsSecond Summer School 2012: 24 trainees, 11 instructors
Third Summer School 2013: 24 trainees, 10 instructors
6
STSM in
2013
FACTS
Slide1919{from COST office} The tangible outputs are preferred. Please, notice that correct use of COST tools
(e.g. well organized meetings) per se is NOT a tangible output, but indeed a tool to achieve your results.
COST aims at supporting Cooperation in Science and Technology Cooperation: Joint operation or
action
COST does not fund research itself
, but provides support for networking activities carried out within COST.Leading a COST Action: an APORIA
COST Action: Just
burning EU money!
Slide2020LABORATORY
In this room Please endeavour to emulate the first five letters and not the last seven.Thank you
Clearly, we can not spend 4 years talking. Even in the best places in Europe.
Slide2121How is it possible to solve this issue?
-1- Convincing people that United we stand
, divided we fall. (in French: L’union fait la force).Most of the teams involved in a COST Action are not rich.
Despite their interest in COST Action, most people are not willing to invest their own money in a collective and anonymous project. No personal credit.
In science if you rare not the first you are nobody! Most of the team expect they will not spend their own funds but at the contrary are hoping to receive money for the join project.
During my talk at the kickoff meeting, I explained that …..100 000 € : 40 = 2500 €
Slide22-2- Giving the minimum for
ACTING!
Most of the participants are experimenters
NOT theorists.
For
instance to perform experiments decided during a WG meeting, at least they need samples or chemicals, especially when they want to improve standardization by performing the same experiment in different laboratories.
We strongly suggest to dedicate
10 - 15
% of the total annual budget
to allow this kind of expenses
.
This should
be consider not as funding research (NO SALARIES, NO INVESTMENT which represent more than 80% of research activity) but helping people involved in a COST Action to do something else than just
burning EU money
and obtain
relevant results
.
Slide2323Third COST
Action TD 1002 Summer School Marcoule, France 1-6
September 201324 trainees 6 Lecturers 5 Trainers young (almost) and excellent gender balance!
The Education
Site, Leader: Alessandro
Podesta, IT
Slide2424Website activity from March 2012 to March 2013
Slide2525