Norm Leppla University of Florida Entomology and Nematology Department Workshop on Breeding and Quality Control of Entomophagous Insects and Entomopathogenic Fungi Merida Mexico ID: 1048697
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1. Total Quality Control for Insect RearingNorm LepplaUniversity of FloridaEntomology and Nematology Department Workshop on Breeding and Quality Control of Entomophagous Insects and Entomopathogenic Fungi Merida, Mexico November 14 – 17, 2017
2. Norm’s Career in Insect RearingUniversity of Arizona- Insect rearing research, 2 years (pink bollworm, noctuids)USDA, ARS- Insectary management, Florida and Texas, 17 years (noctuids, fruit flies, screwworm, parasitoids)USDA, APHIS- Methods development, Washington DC and international, 7 years (gypsy moth, PBW, FF)University of Florida- Integrated pest management and biological control, 20 years Rearing and quality of Tamarixia radiata (Chris Kerr)Quality of Trichogramma brassicae from Ephestia kuehniella eggs (Yasaman Moghaddassi)
3. Quality Control TopicsLearning how to rear quality insectsColony Establishment and MaintenanceMonitoring Insect Quality
4. Learning How to Rear High Quality InsectsApprentice in an insectaryNetwork with other professionalsPublications plus trial and errorVideosVisit other insectariesReviews by expertsEducation and training programs
5. MSU Insect Rearing CenterJohn SchneiderFrank Davis
6. https://www.ncsu.edu/mckimmon/cpe/opd/insectRearing/index.htmlOnline courses Classroom courses Webinars MentoringAllen Cohen
7. Morales-Ramos et al. 2013Cohen 2015Schneider 2009PublicationsVan Lenteren 2009IOBC, MRQA Guidelines and Proceedingswww.amrqc.org
8. Subscribe:(leonwesterd@gmail.com)Insect Rearing Professionals Google GroupLéon WesterdGroup moderatorHead of insect rearingWageningen UR, Netherlands
9. II. Colony Establishment and MaintenancePurposes for Rearing InsectsTypes of Insect Rearing SystemsOptions for Colonizing InsectsMaintaining the Quality of Colonized Insects
10. Hoddle 2015Red Palm WeevilPurposes for Rearing Insects
11. White witch, Thysania agrippina (Lepidoptera: Noctuidae
12. Painted LadySmall-Scale Rearing: ButterfliesTypes of Insect Rearing SystemsMonarch
13. Individual Rearing
14. Medium-Scale Rearing: LepidopteraCabbage Looper, Trichoplusia niCorn Earworm, Helicoverpa zea
15. Small Group RearingKeith HaleinClarence GreenJack RyeBill Fisher
16. Mass Rearing- MedflyMetapa, MexicoEl Pino, GuatemalaCapacity- 3.5 billion sterilemale pupae per week Scott Bauer
17. Mass Rearing- Medfly
18. Options for Colonizing InsectsInsect Rearing Professionals Google Grouphttp://edis.ifas.ufl.eduObtain appropriate biotypes
19. Field CollectingCabbage LoopersTrichoplusia ni
20. Continuous Improvement in Establishing Colonies
21. Maintaining the Quality of Colonized InsectsConsistent procedures (SOPs)Diagnose problemsStrain modificationSam 2005
22. PurposeI. Facilities and EquipmentII. Orange Jasmine CareIII. Asian Citrus Psyllid Rearing IV. Tamarixia radiata Rearing V. Storage and Shipment Appendix (A) Pest IdentificationAppendix (B) Sample Log WorksheetsChris KerrMass-Rearing Tamarixia radiata: Standard Operating Procedures
23. Tritrophic Rearing System Tamarixia radiata SOPsChris Kerr
24. Mass Rearing System for Trichogramma maidisField collect target areaContinuous colonyField InsectaryFluctuating temperature, RHECB hostCorn plants 3-4 meters to fly Release colonyReared 1-3 gen.Lab insectaryConstant temperature, RHFactitious hostNo plantsStored, diapause, monthsRelease parasitized eggs, target area
25. Bill FisherMaintaining Insect Density: Fall Armyworm
26. Changing Procedures: Mediterranean Fruit Fly
27. Soybean Looper vs. Cabbage Looper
28. Diagnose Problems
29. Strain ModificationMaintain “natural” colony- Low productivity, high variability, expensiveSelect in insectary- High productivity, uniformity, consistency, bottlenecksInfuse from field- Short term recovery, hybridize?Hybridize insectary and field populations- Crosses, new strainsReplace colony- New strain, adapt to insectary, low initial productivity
30. Quality Control Criteria and Standardized TestsSampling for Consistent QualityIII. Monitoring Insect Quality
31. Quality Control Criteria and Standardized TestsQuantity- Number of required stageRate of Development- SynchronizationViability- Emergence, sex ratioSize- Weight or measurementsFecundity- Oviposition and egg hatchAdult Behavior- Flight, longevity Field Performance- Achieve purpose
32. Tamarixia radiata Hind Tibia Length and Parasitism on Diphorina citri NymphsAnonymous, UFHind tibia
33. Tamarixia radiata Hind Tibia LengthMinimum, maximum and mean hind tibia length of T. radiata that were developing on different Asian citrus psyllid host nymph instars 1, 2Host instarFemalesMalesMinMaxx̄±SD (n)MinMaxx̄±SD (n)Third----------------0.160.210.20±0.02 (13) cFourth0.230.270.25±0.02 (6) b0.210.270.24±0.02 (55) bFifth0.270.340.31±0.02 (75) a0.270.330.29±0.02 (40) a1 Comparison of means within a given instar were not significantly different between sexes (Tukey’s HSD, α =0.05).Chris Kerr
34. Tamarixia radiata and Diaphorina citri RatiosChris Kerr
35. Level of Tamarixia radiata Parasitism on Diphorina citriChris Kerr
36. Level of Tamarixia radiata Parasitism on Diphorina citriChris Kerr
37. Sampling for Consistent Quality Count = Measurement = N Mean = Sum/Number of Counts = X Variance = Sum of (N – X)2 / n-1 = 2 Standard Deviation = Square Root of 2 = SD
38. ShelfContainer1234561175157.617.4302.761901901911801422167157.69.488.361911911901801763144157.6-13.6184.961911901921891564166157.68.470.561911921901761385136157.6-21.6466.56190191190174181Average157.6190.6190.8190.6179.8158.6Sum of Squares1,113.2Variance278.3Standard Deviation16.70.50.80.95.819.4Number of Cabbage Looper Pupae Per Rearing ContainerShelf 1 is lowest
39. Quality Control Charts
40. Standardized QC TestsJoop van Lenteren (2003), IOBC- Guidelines for quality control of commercially produced natural enemies, 30 speciesRose Buitenhuis (2014), Grower guide to quality assurance of biocontrol products, 22 speciesCustom, develop your own, site-specific
41. For more information or a copy of this presentation please visit: http://ipm.ifas.ufl.edu