Genetic engineering involves inserting foreign genes or modifying the activity of existing genes This technology exploits the process of gene cloning which involves making many identical copies of a gene ID: 920436
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Genetic Engineering (Recombinant DNA technology)
Genetic engineering involves inserting foreign genes or modifying the activity of existing genes.
This technology exploits the process of gene cloning which involves making many identical copies of a gene.
Slide2It has following basic steps:A fragment of DNA, containing the gene to be cloned, is inserted into a circular DNA molecule, to produce a recombinant DNA molecule.
Slide3Slide4(ii) The vector acts as a vehicle that transports the gene into a host cell (Bacteria or other living cells)
Slide5(iii) Within the host cell the vector multiplies, producing numerous identical copies not only of itself but also of gene that it carries.
Slide6Slide7(iv) When the host cell divides, copies of the recombinant DNA molecules are passed to the progeny and further vector replication takes place.
(v) After a number of cell divisions, a clone of identical host cells is produced. Each cell in the clone contain one or more copies of recombinant DNA molecule.
Slide8Slide9Vectors for gene delivery
A DNA molecule capable of replication in a host organism into which a gene is inserted to construct a recombinant DNA molecule.
A vector needs to be relatively small less than 10 kb in size, as large molecule tend to break down during purification and more difficult to manipulate.
Two kinds of DNA molecule that satisfy these criterion can be found in bacterial cells: plasmids and
bacteriophage
chromosomes.