Alkaline Phosphatasealp Quantitative determination of alkaline phosphatase in Serum or heparinized plasma by a colorimetric kinetic method Daheeya AlEnazi Diagnostic Significance ID: 462629
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Slide1
Lab 4Alkaline Phosphatase(alp)
Quantitative determination of alkaline phosphatase in Serum or heparinized plasma by a colorimetric kinetic method.
Daheeya
AlEnaziSlide2
Diagnostic SignificanceHighest elevation of alkaline phosphatase (ALP
) levels in serum :Hepatobiliary disorders and Bone disease. Paget disease' (osteitis deformans). bone cancer.Normal ALP levels are age dependent and are elevated during periods of active bone growth.Slide3
An increase of up to 2-3 times normal is observed In the third trimester of pregnancy. Moderate elevations of ALP (not Involving the liver or bone) may be attributed to 'hodgkins' disease, congestive heart failure, and abdominal bacterial InfectionsSlide4
PRINCIPLEp-Nitrophenylphosphate
+ H2O Alkaline PH p-Nitrophenol + phosphateThe increase of absorbance for P-
nitrophenol
is directly proportional to the amount of ALP present in the sampleSlide5
Procedure
Mix, incubate for 1 minute at 37 CRead initial absorbance at405 nm of the sample, start the the stopwatch and read absorbance at 1 minute intervals thereafter for 3 minutes.
Calculate the difference between
absorbance
and the average absorbance differences per minute (
Δ
A/min)Slide6
CalculationΔA/min
X 2720 = U/L of ALPReference values:Men: 50 - 119 U/L
Women: 43 – 110 U/L