du tissu sain au cancer JeanDamien Combes 1 pour Silvia Franceschi 1 Christine Clavel 2 et Jean Lacau Saint Guily 3 1 International Agency for Research on Cancer Lyon France ID: 541910
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Histoire naturelle de l’infection à papillomavirus humain dans les amygdales : du tissu sain au cancer
Jean-Damien Combes1 pour Silvia Franceschi1, Christine Clavel2 et Jean Lacau Saint Guily31 International Agency for Research on Cancer, Lyon, France2 INSERM UMR-S 903 / Université de Reims Champagne-Ardenne / CHU Reims, Laboratoire Pol-Bouin, Reims, France3 Service d'ORL et chirurgie cervico-faciale, Hôpital Tenon AP-HP, Paris, FranceSlide2
Study of human Papillomavirus and precancerous Lesions
In the TonsilSPLIT studyMethods- Cross-sectional study- 20 centres in France (2012-2016)- Tonsillectomy for non-cancer reasons- Gargling specimens before surgery- Detection of infectious agents- Cytological and histological precancerous lesionshttp://split.iarc.fr/Slide3
1. HPV infection in non-cancerous tonsils
2. HPV-induced lesions in the oropharynxSlide4
HPV infection in non-cancerous tonsils
Sample testedAge classM/FTotal
0-14
15-24
25-34
35+
Tonsil brushing
139
169
191
193
314/378692Gargling-899089121/147268
HPV-DNA detection test: Luminex® IARC E7-MPG (21 HPV types) (T. Gheit, M. Tommasino)
4Slide5
HPV infection in non-cancerous tonsils
SPLIT study final results (submitted)HPV DNA detection test: IARC E7-MPG platformSlide6
HPV infection in non-cancerous tonsils,by age class and sex (gargle)
SPLIT study final results (submitted)HPV DNA detection test: IARC E7-MPG platformSlide7
HPV infection in non-cancerous tonsils,HPV types
SPLIT study final results (submitted)HPV DNA detection test: IARC E7-MPG platformSlide8
HPV infection in non-cancerous tonsils,concordance Gargle/Tonsil brushing
SPLIT study final results (submitted)HPV DNA detection test: IARC E7-MPG platformSlide9
1. HPV infection in non-cancerous tonsils
2. HPV-induced lesions in the oropharynxSlide10
HPV-induced lesions in
the tonsil (cytology)Slide11
HPV-induced lesions in the tonsil (cytology)
Uninterpretable cytology: many clustered lymphocytes hiding epithelial cells
Uninterpretable
cytology
:
low
cellularitySlide12
HPV-induced lesions in the tonsil (cytology)
PMID: 26111735Slide13
HPV-induced lesions in the
tonsil (histology)Slide14
ConclusionsPrevalence of tonsillar HPV infection is overestimated using garglingPoor concordance between HPV detection by gargle and tonsil brushing
Limited prospect for early detection of precancerous lesion in the tonsils HPV-induced tonsillar precancerous lesions?Slide15
AcknowledgementCoordination méthodologie : Jean-Damien Combes (CIRC) et Véronique Dalstein (Reims)
Coordination anatomo-pathologie : Philippe Birembaut (Reims) et Marine Lefèvre (Tenon)Coordination biologie moléculaire : Alain Jung (Paul Strauss Strasbourg) et Christine Clavel (Reims)Coordination épidémiologie et statistiques : Gary Clifford (CIRC) et Silvia Franceschi (CIRC)Coordination chirurgie : Jean Lacau Saint Guily (Tenon) Chirurgie ORL : Olivier Mauvais et Jean-Michel Badet (Besançon), Emmanuel Babin (Caen), Christian Righini (Grenoble), Justine Lerat (Limoges), Philippe Céruse et Alain Cosmidis (Lyon), Renaud Garel (Montpellier), Olivier Malard (Nantes), Benjamin Lallemant (Nîmes), Nicolas Leboulanger (Trousseau, Necker), Christine Bach (Foch), Sébastien Albert et Beatrix Barry (Bichat), Vincent Couloigner (Necker), Natacha Teissier et Thierry Van Den Abeele (Debré), Bertrand Baujat, Olivier de Crouy Chanel, Maria Lesnik et Sophie Périé (Tenon), Xavier Dufour (Poitiers), Jean-Claude Mérol (Reims), Philippe Schultz (Strasbourg), Sébastien Vergez (Toulouse), Sylvain Morinière (Tours). Anatomo-pathologie : Elisabeth Brambilla (Grenoble), Béma Coulibaly (Limoges), Françoise Berger (Lyon), Elisabeth Cassagnau (Nantes), Heliette Chapuis (Nîmes), Dibor Niang et Aurore Coulomb (Trousseau), Isabelle Wagner (Foch), Louise Galmiche-Rolland (Necker), Dominique Berrebi (Debré), Alexandra Traverse-Glehen (Lyon), Isabelle Brocheriou (Tenon), Pierre Levillain et Eric Frouin (Poitiers), Marie-Danièle Diebold (Reims), Mihaela Onea (Strasbourg), Emmanuelle
Uro-Coste (Toulouse).Anatomo-pathologie SPLIT Lésions précancéreuses : Yves Denoux (Foch), Muriel Hourseau (Bichat), Emmanuèle
Lechapt
(Caen), Cécilia Rousselot-Denis (Tours), Valérie Costes (Montpellier), Séverine
Degano-Valmary
(
Besançon).
Biologie
: Tarik Gheit et Massimo Tommasino (CIRC), Christiane
Mougin et Jean-Luc Prétet (Besançon), Roger Lacave et Patrick Soussan (Tenon), Brigitte Autran et Ghislaine Carcelain (Pitié), Gérard Agius et Agnès Beby-Defaux (Poitiers), Joseph Abecassis (Paul Strauss Strasbourg).Financement PAIR-VADS 2011 – Fondation ARC / INCa / Ligue contre le cancer