MODEL Jeremy Sprouse 1 DDS Chethan Sampath 2 PhD Pandu R Gangula 2 PhD School of Graduate Studies and Research 1 Department of ODS amp Research 2 Meharry Medical College School of Dentistry Nashville TN 37208 ID: 927565
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Slide1
ROLE OF SEX HORMONES AND THEIR RECEPTORS ON GASTRIC NRF2 AND NEURONAL NITRIC OXIDE SYNTHASE FUNCTION IN AN EXPERIMENTAL HYPERGLYCEMIA MODEL
Jeremy Sprouse1, DDS, Chethan Sampath2, PhD, Pandu R Gangula2, PhDSchool of Graduate Studies and Research1, Department of ODS & Research2, Meharry Medical College, School of Dentistry, Nashville, TN 37208
Supplementary Data
Slide2β
-Actin 43 kD
Fig. 3 A
nNOS
α
155
kD
+ICI 182780
NG
+ICI 182780
+ ru-486 (100
nM
)
+ ru-486 (100
nM
)
+ICI 182780
NG
+ICI 182780
+ ru-486 (100
nM
)
+ ru-486 (100
nM
)
Slide3Fig. 3 B - C
Er α 67
kD
+ICI 182780
NG
+ICI 182780
+ ru-486 (100
nM
)
+ ru-486 (100
nM
)
Nrf2 57
kD
+ICI 182780
NG
+ICI 182780
+ ru-486 (100
nM
)
+ ru-486 (100
nM
)
+ICI 182780
NG
+ICI 182780
+ ru-486 (100
nM
)
+ ru-486 (100
nM
)
β
-Actin 43
kD
Slide4Fig. 3 D-E
HO-1 32 kD
β
-Actin 43
kD
ER
β
57
kD
+ICI 182780
NG
+ICI 182780
+ ru-486 (100
nM
)
+ ru-486 (100
nM
)
+ICI 182780
NG
+ICI 182780
+ ru-486 (100
nM
)
+ ru-486 (100
nM
)
+ICI 182780
NG
+ICI 182780
+ ru-486 (100
nM
)
+ ru-486 (100
nM
)
Slide5Fig. 6A
nNOS α
155
kD
NG
50
mM
Mannitol
3
0
mM
HG
50
mM
HG
NG
50
mM Mannitol
30 mM HG
50
mM HG
Slide6Fig. 6B
β-Actin 43
kD
Nrf2 57
kD
NG
50
mM
Mannitol
3
0
mM
HG
50
mM
HG
NG
50
mM
Mannitol
3
0 mM HG
50 mM HG
Slide7Fig. 6C
β-Actin 43
kD
Er
α
67
kD
NG
50
mM
Mannitol
3
0
mM
HG
50
mM HG
NG
50
mM Mannitol
3
0 mM HG
50 mM HG
Slide8nNOS
α 155 kD
Fig. 6 D
β
-Actin 43
kD
HG
NG
HG + PPT (10
nM
)
HG + PPT (100
nM
)
HG + DPN (1.0
μ
M)
HG + DPN (10
μ
M)
HG
NG
HG + PPT (10
nM)
HG + PPT (100
nM
)
HG + DPN (1.0
μ
M)
HG + DPN (10
μ
M)
Slide9Fig. 6 E-F
β-Actin 43 kD
HG
NG
HG + PPT (10
nM
)
HG + PPT (100
nM
)
HG + DPN (1.0
μ
M)
HG + DPN (10 μM)
ER
α
67 kD
HG
NG
HG + PPT (10
nM
)
HG + PPT (100 nM)
HG + DPN (1.0 μM)
HG + DPN (10
μ
M)
Nrf2
5
7
kD
HG
NG
HG + PPT (10
nM
)
HG + PPT (100
nM
)
HG + DPN (1.0
μ
M)
HG + DPN (10
μ
M)
Slide10Fig. 6 G-H
Er
β
57
kD
HO-1 32
kD
β
-Actin 43
kD
HG
NG
HG + PPT (10
nM
)
HG + PPT (100
nM
)
HG + DPN (1.0
μ
M)
HG + DPN (10 μM)
HG
NG
HG + PPT (10
nM
)
HG + PPT (100
nM
)
HG + DPN (1.0
μ
M)
HG + DPN (10
μ
M)
HG
NG
HG + PPT (10
nM
)
HG + PPT (100
nM
)
HG + DPN (1.0
μ
M)
HG + DPN (10
μ
M)
Slide11Fig. 7A
β-Actin 43
kD
nNOS
α
155
kD
+E
2
10
nM
+P
4
100
nM
NG
50
mM
Mannitol
30 mM HG
50
mM HG
+E2 100 nM
+P
4
1000
nM
+E
2
10nM P
4
100
nM
+E
2
10 nM+P
4
100
nM
+E
2
10
nM
+P
4
100
nM
NG
50
mM
Mannitol
3
0
mM
HG
50
mM
HG
+E
2
100
nM
+P
4
1000
nM
+E
2
10nM P
4
100
nM
+E
2
10 nM+P
4
100
nM
Slide12Fig. 7B
β
-Actin 43
kD
Nrf2 57
kD
+E
2
10
nM
+P
4
100
nM
NG
50
mM
Mannitol
3
0 mM HG
50 mM HG
+E2
100 nM
+P
4
1000
nM
+E
2
10nM P
4
100
nM
+E
2
10 nM+P
4
100
nM
+E
2
10
nM
+P
4
100
nM
NG
50
mM
Mannitol
3
0
mM
HG
50
mM
HG
+E
2
100
nM
+P
4
1000
nM
+E
2
10nM P
4
100
nM
+E
2
10 nM+P
4
100
nM