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Green  OK but look at other factors Blue  bad Black  really ba Green  OK but look at other factors Blue  bad Black  really ba

Green OK but look at other factors Blue bad Black really ba - PDF document

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Uploaded On 2022-08-16

Green OK but look at other factors Blue bad Black really ba - PPT Presentation

Nucleotide sequence of DNA isolated from Chilean Blob TAATACTAACTATATCCCTACTCTCCATTCTCATCGGGGGTTGAGGAGGACTAAACCAGACTCAACTCCG AAAAATTATAGCTTACTCATCAATCGCCCACATAGGATGAATAACCACAATCCTACCCTACAATACAACC AT ID: 937393

blast match hit form match blast form hit 2012 august david bad link sequence

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Presentation Transcript

Green = OK, but look at other factors ¥!Blue = bad ¥!Black = really bad! 2. How long are the matched segments? Longer = better

David Form - August 15, 2012 19 The hit list ¥!BLAST lists the best matches (hits) Ð!For each hit, BLAST provides: ¥!Accession

number Ð links to Genbank flatfile Description ¥!G = genome link ¥!E-value Ð!An indicator of how good a match t

o the query sequence ¥!Score Ð!Link to an alignment David Form - August 15, 2012 20 What is an E-value? ¥!E-value Ð!The chance t

hat the match could be random Ð!The lower the E-value, the more significant the match ¥!E = 10-4 is considered the !Nucleotide

sequence of DNA isolated from Chilean Blob. TAATACTAACTATATCCCTACTCTCCATTCTCATCGGGGGTTGAGGAGGACTAAACCAGACTCAACTCCG AAAAATTATAGCT

TACTCATCAATCGCCCACATAGGATGAATAACCACAATCCTACCCTACAATACAACC ATAACCCTACTAAACCTACTAATCTATGTCACAATAACCTTCACCATATTCATACTATTTATCCAAAACT

CAACCACAACCACACTATCTCTGTCCCAGACATGAAACAAAACACCCATTACCACAACCCTTACCATACT TACCCTACTTTCCATAGGGGGCCTCCCACCACTCTCGGGCTTTATCCCCAAATGAA

TAATTATTCAAGAA CTAACAAAAAACGAAACCCTCATCATACCAACCTTCATAGCCACCACAGCATTACTCAACCTCTACTTCT ATATACGCCTCACCTACTCAACAGCACTAACCCTATTCCCCT

CCACAAATAACATAAAAATAAAATGACA ATTCTACCCCACAAAACGAATAACCCTCCTGCCAACAGCAATTGTAATATCAACAATACTCCTACCCCTT ACACCAATACTCTCCACCCTATTATAG