1000 1000 1000 1000 mm Micrometre n m Nanometre Micrometre Millimetre 5 0005 0000005 1 1 1 3 7 05 0001 0000001 1000 ID: 631790
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Slide1
Microscopy and MagnificationSlide2
1000
1000
1000
1000
mm
Micrometre
n
mSlide3
Nanometre
Micrometre
Millimetre
5
0.005
0.000005
1
1 1 3 7 0.5
0.001
0.000001
1000
0.001
1 000 000
1000
3000
0.003
0.007
0.000007
500 000
500Slide4
The difference between
magnification and resolution.
Magnification is the degree to which the size of an image is larger than the image itself.
Resolution is the degree to which it is possible to distinguish between two objects that are very close together.Slide5
How do we find the overall magnification of a light microscope?
Eyepiece Magnification
Objective Magnification
Overall Magnification
X10
X4
X10
X10
X10
X40 X10X100 EyepieceObjective lens401004001000Slide6
The diagram below is a drawing of an organelle from a ciliated cell as seen with an electron microscope.
Calculate
the actual length of the organelle as shown by the line AB in the diagram. Express your answer to the nearest
micrometer
(mm).
Show your working. Answer = ...........................................
mm
A =
I
M
=102mm20000=102000mm200005.1Slide7
The
diagram below is a drawing of an alveolus together with an associated blood capillary.
The
line
AB in the diagram represents an actual distance of 1.5 µm.Calculate the magnification of the drawing. Show your working.
Answer = × .................................................
M =
I
A
=21mm1.5mm=21000mm1.5mm14000Slide8
The diagram below shows the general structure of an animal cell as seen under an electron microscope
.
_________
5
m
1) Calculate
the magnification factor of the diagram
M =
I
A=24mm5mm=24000mm5mm= 4800Slide9
The diagram below shows the general structure of an animal cell as seen under an electron microscope
.
_________
5
m
2
) Calculate the actual length of structure G
A
=
IM=12mm4800=12000mm4800= 2.5mmSlide10
The diagram below shows the general structure of an animal cell as seen under an electron microscope
.
_________
5
m
3)
Calculate the diameter of the nucleolus (structure B)
A
=
IM=8mm4800=8000mm4800= 1.666mm= 1.7mmSlide11
The diagram below shows the general structure of an animal cell as seen under an electron microscope
.
_________
5
m
4)
Calculate the diameter of the nucleus
A
=
IM=36mm4800=36000mm4800= 7.5mmSlide12
The diagram below shows the general structure of an animal cell as seen under an electron microscope
.
_________
5
m
5
) Calculate the diameter of the cell at its widest point
A
=
IM=116mm4800=116000mm4800= 24.16666mm= 24.2mmSlide13
The diagram below shows the general structure of a plant cell when viewed under and electron microscope.
___________
40
m
1) Calculate the magnification factor of the
diagram
M =
IA=23mm40mm=23000mm40mm= 575Slide14
The diagram below shows the general structure of a plant cell when viewed under and electron microscope.
___________
40
m
2) Calculate the thickness of the cellulose cell wall.
A
=
IM=3mm575=3000mm575= 5.2mmSlide15
The diagram below shows the general structure of a plant cell when viewed under and electron microscope.
___________
40
m
3) Calculate the length of the cell
.
A
= IM=98mm575=98000mm575= 170.4mmSlide16
The diagram below shows the general structure of a plant cell when viewed under and electron microscope.
___________
40
m
4) Calculate the length of structure C.
A
=
IM=24mm575=24000mm575= 41.7mmSlide17
The diagram below shows the general structure of a plant cell when viewed under and electron microscope.
___________
40
m
5) Calculate the length of the vacuole.
A
=
IM=71mm575=71000mm575= 123.5mmSlide18
M =
I
A
=
82mm
20
m
m
=
82000
mm20mm= 4100Calculate the actual length of structure C.Show your working and give your answer in micrometres (μm).Slide19
M =
I
A
=
82mm
20
m
m
=
82000
mm20mm= 4100Calculate the actual length of structure C.Show your working and give your answer in micrometres (μm).A = IM=15mm4100=15000mm4100= 3.6mmSlide20
Take a calculator and a clear ruler into Biology exams
Always show the working out
Make sure correct units are added