Berger B Knapp P Gallnböck M SZV OÖ SZV OÖ 27 th Annual Meeting of DAGENE Germany Introduction Frozen boar semen since gt 40 years Thalheim gt 25 ID: 611488
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Slide1
Backup not only for computers!
Berger B. Knapp P. Gallnböck M.
© SZV OÖ
© SZV OÖ
27
th
Annual Meeting
of
DAGENE, Germany
Slide2
Introduction
Frozen boar semen – since
> 40 yearsThalheim > 25 years Special uses – not for routine AIScientificExportTransport Planned
mating
Genebank – e.g. backup for
cattle AI since 1997Mangalica, Turopolje
in Austria since 2000Slide3
Frozen boar
semenAbout 30% of boars
not freezableNon-return rates not really satisfying Smaller litter size Huge
amount of
sperm cellsLess
if deep intra-uterine inseminationHandling
of semen
more complicated Processing and
freezing Thawing and re
-dilution…but long-time storage is
possible!Slide4
Why another
cryo-project?Biggest Austrian pig
AI-centre 8 km from Genebank 270 boars, 5 breeds, PRRS-free statusOwned
by pig
breeding associationPRRS outbreak
2012, all boars slaughteredHuge
financial loss
to centre and
producersRe-stocked boars not first
qualityLoss of breeding progress
Safeguard against loss of Top-Genetics neededSlide5
The project
Cooperation between Pig
Breeders Association and GenebankScreen all TOP-Genetics boars for freezability
of semen
Conserve freezable boars
in GenebankDo not disrupt
semen
collection routine at AI centre!Slide6
Material & Methods
1Screening: 62 ejaculates, 28 TOP-
boars (15 Piétrain, 7 Landrace, 4 Large White, 1 Duroc, 1 Schwäbisch-Hällisch) Collection within routine of
AI centre,
20ml of sperm-rich fraction
of ejaculateDilution 1:1 with BTS
extender (DiluPorc
TM)Fresh
semen analysis CASA (SpermVision,
minitube): Motility (mot
), DensityTransport to GenebankAcrosome integrity (acr
) – smears stained acc. KOVACS & FOOTE (1992)Slide7
Material & Methods 2
Cryo-conservation at Genebank acc
. WESTENDORF et al. (1975) mod. EGERSZEGI et al. ( 2009)Extender Androstar Cryo Plus (minitube)0,5 ml medium French straws,
concentration 1x109
cells/mlThawing at AI centre
37°C for 20 sec.Re-dilution with BTS-extender
, CASA, smears
Minimal requirements: 30% intact acr, 30%
progressive motConservation: 5 to 9 double AI
doses/boarOne whole
ejaculate neededSlide8
Results – Screening
fresh
thawed
62
ejaculates
28
boars
70%
blocks
Minimum/Maximum
Median
acr
mot
30%
ejaculates
discarded
!
3
boars
not
freezable
!
Slide9
Results – Genebanking
fresh
thawed
25
ejaculates
25
boars
0
ejaculates
discarded
!
All
boars
freezable
!
Slide10
Discussion
Success rate in line
with literatureStill 30% negative ejaculates in screeningStill 13 boars at least 1 negative resultFreezability of
semen Individual
trait – genetic determination?Fresh
semen quality allows
no predictions!
Sexual resting time important (< 5 days
)5 x 109 sperm cells per dose!
Much semen needed for
Cryo (one whole ejaculate)Intra-uterine insemination not used by breeders
rfSlide11
Conclusions
Enhanced security for breeders and
breeders associationScreening well integrated into routine Backup collection owned
by breeders
associationstored at Genebank (extra tank)
for 5 yearsAfter 5 years 2 double
doses into
core collection – owned by
state, rest destroyedExport
orders pendingSlide12
Thank
you
for listening!11 piglets
*
10.09.2014
from semen
frozen
17.07.1993!