Dr Richy Hetherington Prof Rob Pickard and Dr Simon Cockell Outline Recording Research in a Clinical Setting Recording Lab Work Research Diaries Electronic recording Sharing information What you should share and how to share it ID: 236110
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Slide1
Recording Your Research (Lab Books, Research Diaries and Electronic Information)
Dr Richy Hetherington,
Prof Rob Pickard
and Dr Simon CockellSlide2
Outline
Recording Research in a Clinical Setting
Recording
Lab
Work
Research Diaries
Electronic
recording
Sharing information – What you should share and how to share itSlide3
Why do you need to keep records?
For Your Progress Panel and
Viva
For Your Supervisory Team
For Anyone Who Continues Your Work
For Academic Integrity
For Intellectual Property
For Your Sponsors
Most of all for you: Writing your thesis and your publications will be easier the better your records areSlide4
Which Standards?
http://www.mhra.gov.uk/Howweregulate/Medicines/Inspectionandstandards/GoodLaboratoryPracticeSlide5
University Lab Books
All should be
Have continuous numbered pages
Have a table of contents
Entries should be made permanently in Pen
No correcting fluid or tipex should be used
A single line should be put through any errorsSlide6
When to record
Record as you go
If you can’t do it as soon as you can
It is easy to
forget
Do not record on scraps of paperSlide7
New Methods
Always describe thoroughly a new procedure
For example
“
Solution B was made by adding 500µl of water to 1.02mg of substance B, in 1.5ml microfuge tube, the solution was vortexed for 30 minutes to ensure Substance B was dissolved
.
Would be better than
“
A 2mg/ml of Solution B was made from substance B
”Slide8
Established Methods
Once methods are established it is fine to refer back to them
Eg.
“
Samples B1-B16 were tested using method A (see 14th June 2007
RH/B1/ p14
)
”
Obviously note any deviations, intentional or otherwiseSlide9
Recording Deviation
E.g. (accident)
“Samples B1-B16 were tested using method A (see 14th June 2007
RH/B1/p14
). Deviating from the method Samples B13-B16 were centrifuged twice at 14.2g for 5 minutes in stage 2,
with a 5 minute interval between spins
all
other samples were centrifuged once as per method A
.”
Or
E.g. (design)
“
Samples B1-B16 were tested using method A (see 14th June 2007
RH/B1/ p14
). Deviating from the method all samples were centrifuged at 14.2g for 10 minutes at stage 2, to ensure a compact pellet formed.Slide10
Equipment, Reagents and Samples
Equipment used list
Equipment name
Manufacturer
Model Number
Serial number
Date used
notes
Centrifuge 1
Sigma
2-16
445332
24 June 2008-
Normally fitted with Rotor A, takes 3 minutes to get to top speed at highest ramp rate
Rotor A
Sigma
11124
545434
24 June 2008-
Fits 24 microfuge tubes
Reagents used list
Lab name
Full Name
Supplier
Catalogue Number
Lot
number
Dates usednotesPEG600/1Polyethylene Glycol 600Polysciences inc.http://www.polysciences.com/25322-68-334543542 May 2008to3 July 2008Went cloudy 8 July 08 ordered new stockPEG600/2Polyethylene Glycol 600Polysciences inc.http://www.polysciences.com/25322-68-334545539 July 2008toSlide11
Recording the results
Make note of anything anecdotal or unusual.
“
The cells appeared to be more irregular in shape, than in previous experiments
”
“The interview room was very warm; some of the subjects seemed slightly unconformable
”Slide12
Seemingly unimportant things may become important later
Eg
“Centrifuging for 10 minutes at stage 2 had no perceivable effect on the compactness of the pellet. When repeating increasing the rotor speed to produce 15g may help compact the pellet”
…later…
“
Excessive centrifuging of samples B1-16 reduced enzyme activity, solids in stage 2 must be collected by filtering
”Slide13
Results from machines
Annotate Well
Reference page
Number the reverse
Stick in with sticky tapeSlide14
Intellectual Property (IP) Issues
Lab books must be signed off
(at least fortnightly)
All experiments will need to be reproducible
Lab book will become a legal document if a patent is applied forSlide15
Faculty Lab books
For PhD, MD &
Mphil
student Lab books Should be available through your institute Office
For
MRes
students from the Graduate School Office