Johnson DJ Ostlund EN Pedersen DD Schmitt BJ Detection of North American West Nile Virus in Animal Tissue by a Reverse TranscriptionNested Polymerase Chain Reaction Assay Emerg Infect Dis 200174739741 httpsdoiorg103201eid0704017425 ID: 1026956
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1. Figure 2Figure 2. . Visual comparison of first stage reverse transcription-polymerase chain reaction (RT-PCR) amplification products with RT-nested (n) PCR amplification products. Lanes 1-9 represent first-stage products of 10-fold dilutions 10-1 through 10-9. Lanes 10-18 represent nested amplification products of same dilutions. L: 100-bp DNA ladder.Johnson DJ, Ostlund EN, Pedersen DD, Schmitt BJ. Detection of North American West Nile Virus in Animal Tissue by a Reverse Transcription-Nested Polymerase Chain Reaction Assay. Emerg Infect Dis. 2001;7(4):739-741. https://doi.org/10.3201/eid0704.017425