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Tetrazolium Tetrazolium

Tetrazolium - PowerPoint Presentation

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Tetrazolium - PPT Presentation

testing referee results Southwest Region 4 2016 by Michael Aberle RST Ransom Seed Laboratory Tetrazolium testing referee results 2016 40 participants 30 laboratories 35 of the respondents were from the West coast region 4 ID: 550725

respondents viable concern answered viable respondents answered concern 100 embryo unstained flowering asteraceae oleracea sunflower helianthus brassica kale brassicaceae

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Slide1

Tetrazolium testing referee resultsSouthwest Region 42016

by Michael

Aberle

RST

Ransom

Seed LaboratorySlide2

Tetrazolium testing referee results2016

40 participants

30 laboratories

35%

of the respondents were from the West coast region 4

20 photographs

8 plant families Slide3

1. BLUE FLAX

Linum

lewisii

Linaceae

98% viable

Respondents that answered viable:

Concern that the radicle was damagedSlide4

2. BLUE

FLAX

Linum

lewisii

Linaceae

100% non-viableSlide5

3. BLUE

FLAX

Linum

lewisii

Linaceae

98% non-viable

Respondents that answered viable:

Concern that a membrane covered the unstained partsSlide6

4

. CACTUS

Cactaceae

100% non-viableSlide7

5

. CACTUS

Cactaceae

95%

viable

Respondents that answered non-viable:

Concern that there was a crack in the embryoSlide8

6. Flowering kale

Brassica

oleracea

Brassicaceae

52%

viable

Respondents that answered non-viable:

Concern that necrosis reached the juncture of the cotyledons and the embryo axisSlide9

7. Flowering

kale

Brassica

oleracea

Brassicaceae

90%

viable

Respondents that answered non-viable:

Concern that the radicle was broken

Concern that there was insufficient staining Slide10

8. Flowering

kale

Brassica

oleracea

Brassicaceae

87

%

non-viable

Respondents that answered viable:

Concern that the necrosis is only superficial

Embryo axis is not damaged

Non-essential areas unstainedSlide11

9. Flowering

kale

Brassica

oleracea

Brassicaceae

100% non-viableSlide12

10. LOVAGE

Levisticum

officinale

Apiaceae

82% non-viable

Respondents that answered viable:

Concern that embryo might have a light stain

Could be dormant as in AOSA/SCST TZ Handbook where there is the case of an unstained embryo with stained endosperm Slide13

11. LOVAGE

Levisticum

officinale

Apiaceae

100%

viableSlide14

12. NASTURTIUM

Tropaeolum

majus

Tropaeolaceae

95% non-viable

Respondents that answered viable:

Concern that the embryo stained lightly Slide15

13. NASTURTIUM

Tropaeolum

majus

Tropaeolaceae

65% non-viable

Respondents that answered viable:

Could not relate the image to the example given in the AOSA/SCST TZ Handbook

Unstained areas are minorSlide16

14. OREGANO

Origanum

vulgare

Lamiaceae

98% non-viable

Respondents that answered viable:

Saw uniform stainSlide17

15. OREGANO

Origanum

vulgare

Lamiaceae

100%

viableSlide18

16.

Phormium

sp.

Xanthorrhoeaceae

[

Agavaceae

]

100%

viableSlide19

17.

Phormium

sp

.

Xanthorrhoeaceae

[

Agavaceae

]

98% non-viable

Respondents that answered viable:

Saw more than half the embryo stainedSlide20

18.

Phormium

sp.

Xanthorrhoeaceae

[

Agavaceae

]

95%

viable

Respondents that answered non-viable:

Concerned that the endosperm was unstained (light reflecting off the seed made it look unstained)Slide21

19. SUNFLOWER

Helianthus

annuus

Asteraceae

90% non-viable

Respondents that answered viable:

Concern that the damage at the root tip was minimal

More than half the embryo stained

In the AOSA/SCST TZ Handbook slight damage to the root tip is acceptable Slide22

20. SUNFLOWER

Helianthus

annuus

Asteraceae

90%

viable

Respondents that answered non-viable:

Concern over the dark spots on the radicle

Unstained cotyledon Slide23

21. SUNFLOWER

Helianthus

annuus

Asteraceae

100% non-viableSlide24

22. SUNFLOWER

Helianthus

annuus

Asteraceae

100% non-viable

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