Structure Central Dogma Template Strand Coding Strand RNA Functions RNA Building Blocks Structure Eukaryotic Process RNA Polymerase Subunits Prokaryotes α 2 β β ω σ Transcription ID: 654724
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Slide1
Transcription
Dr. Kevin AhernSlide2
StructureSlide3
Central Dogma
Template Strand
Coding StrandSlide4
RNA FunctionsSlide5
RNA Building BlocksSlide6
Structure
Eukaryotic ProcessSlide7Slide8
RNA Polymerase
Subunits (Prokaryotes)
α (2)
β
β’
ω
σSlide9
Transcription
Template Strand
Non-template Strand
RNA
Polymerase
RNASlide10
Prokaryotic Control SequencesSlide11
Prokaryotic RNA Polymerase (RNAP)Slide12
StructureSlide13
Transcription Initiation and Elongation
Transcription Bubble
Rifampicin Blocks
ElongationSlide14
Transcription ElongationSlide15
Transcription TerminationSlide16
StructureSlide17
Termination
Rho Factor Termination - Rho Protein “Climbs” RNA Being Synthesized and Removes RNA Polymerase from DNA
Factor Independent - Sequence in RNA Causes RNA Polymerase to Fall Off of DNASlide18
Chromosome StructureSlide19Slide20
Eukaryotic Control Sequences & StructureSlide21
Eukaryotic Transcription ComplexSlide22
Transcription Complex AssemblySlide23Slide24
Eukaryotic RNA Polymerase Inhibition
α-amanitinSlide25
Eukaryotic mRNA 5’ CapSlide26
Eukaryotic mRNA 5’ CapSlide27
Eukaryotic RNA MaturationSlide28
SplicingSlide29
SplicingSlide30
Splicing
GU Sequence
AG Sequence
1. U1 Binding
2. U2 Binding
Pyrimidine Rich SequenceSlide31
RNA
Splicing
snRNPs - Contain Proteins and snRNAs.
Some snRNAs are Complementary
to Sequences in Intron and
Help to Assemble the StructureSlide32
Splicing MechanismSlide33
Alternative SplicingSlide34
Alternative PolyadenylationSlide35
RNA EditingSlide36
StructureSlide37
tRNA Structure
CCA EndSlide38
tRNA
Synthesis of Pseudouridine from Uridine Slide39
tRNA and rRNA Processing in EukaryotesSlide40
StructureSlide41
StructureSlide42
Structure
Bacteria
5S rRNA (in large ribosomal subunit - structural support)
16S rRNA (in small ribosomal subunit - aligns mRNA for translation)
23S rRNA (in large ribosomal subunit - catalyzes peptide bond formation
Eukaryotes
5S rRNA (large ribosomal subunit - structural support)
5.8S rRNA (large ribosomal subunit - translocation)
18S rRNA (small ribosomal subunit - functions in translation)
28S rRNA (large ribosomal subunit - peptide bond formation)Slide43
Structure
RNA Functions
Synthesis of Proteins (tRNA, rRNA, mRNA)
Primary Genetic Material (RNA Viruses)
Catalysis of Reactions (Ribozymes)
Regulation of Gene Expression (miRNA & siRNA)
Processing of Other RNAs (snRNAs & snoRNAs),
RNA Structures
Ribonucleotides - A,U,C,G
Single Stranded/Self Pairing
G-U Base PairsSlide44
Structure