GCS MINI GRANT 20122013 Christal MacLamroc Welborn Academy DNA Extraction Procedure for Strawberry DNA 1 Place 1 strawberry in the sandwich bag and push out the air 2 Using your hands mash the strawberry through the bag ID: 722956
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Slide1
"Wait! What's in my Mouth?"
GCS MINI GRANT 2012-2013Christal MacLamrocWelborn Academy Slide2
DNA Extraction
Procedure for Strawberry DNA1) Place 1 strawberry in the sandwich bag and push out the
air. 2) Using your hands mash the strawberry through the bag.3) Add two droppers full of DNA extraction solution and continue to mash the
strawberry with your hands.4) Cut a corner in the bag, place the gauze over the cup and press the liquids into your test tube.5) Add
a dropper full of
isopropyl alcohol
to the test
tube.
DO NOT MIX THE
SOLUTION IN THE TEST
TUBE.
Procedure
for
Cheek cell DNA:
1) Use
a toothpick and
LIGHTLY
scratch the inside of your cheeks.
2) Swirl
water in your cheek
DO NOT DRINK IT
and spit the water into a cup.
3) Empty
the contents of the cup into a test tube.
4) Add
two droppers full of DNA extraction solution into the test tube and mix.
5) Add
a dropper full of
isopropyl alcohol
to the test
tube.
DO
NOT MIX
THE SOLUTION IN THE
TEST TUBE.Slide3
Looking at Cheek Cells
What is DNA???Slide4
Extracting S
trawberry Cells
Does a Strawberry have DNA?
Add DNA Extraction Liquid to break open the strawberry cells Slide5
After adding the isopropyl alcohol we wait for the DNA to separate.
After mashing the strawberries they had to be strained.
Hard at work…Slide6
SCIENCE IS AWESOME!!
YUCK!Slide7
We have DNA!
DNA
Slide8
Specifics of project including creative aspects:
All living things are made up of DNA. DNA, however, is very small and difficult to see. This activity will allow students the ability to explore and visualize DNA in various different living organisms including themselves. In the lab, students will physically extract DNA from strawberries, bananas, kiwis, and their own cheek cells. Students will begin by mixing and creating our lysis solution, which breaks up DNA. The solution is 2L-distilled water, 100-mL detergent and 15g of salt. Using our lysis solution and by adding rubbing alcohol each student will be able to “see” DNA clumps from each fruit and their own cheek cells. Our lab will relate the concept of DNA and living organisms and will provide the groundwork for understanding genetics.
What you expect the project to accomplish?I want to provide my students with a distinctive and memorable experience about DNA, which they’ve often heard about but probably never seen. I hope this lab will provide a solid foundation in their Science education and that this inquiry based hands on approach will result in improved performance in the classroom. What are the long-term benefits of the project?
Through this lab, experiments will gain a first glance at DNA and it will not be from a picture found in a book. In addition, they will learn the knowledge necessary to conduct basic laboratory techniques.Total amount of money required: $300
Itemization
of amount:
Palmolive washing detergent 25oz $3.99
Salt 30g $6.99
Fisher brand
Kimax
Reusable Test Tubes (pack of 72) $210
Thermo Scientific Nalgene Droppers (pack of 100) $59.53
70% Isopropyl Rubbing Alcohol 100ml $10
Total= 290.51
Grade level:
7
Number of students served: 170+Currently no other funding is available for this project
.
Wait! What’s in my Mouth?Slide9
THANK YOU GCS Mini Grant