Lot ________ Expiry Date _______C A T G AA G T A CConcentration - PDF document

1 Lot: ________ Expiry Date: _______C A
Lot: ________ Expiry Date: _______C A T G AA G T A CConcentration: 10 U/µL gene from Sau 14-027 Supplied with: 1 mL of 10X Buffer O digest 1 µg of lambda DNA in 1 hour at 37°C in 50 µL of Please go to www.thermoscientific.com/doubledigest choose the best buffer for your experiments. nuclease-free water 16 µL 10X Buffer O 2 µL DNA (0.5-1 µg/µL) 1 µL PagI 0.5-2 µL PCR reac

2 tion mixture 10 µL nuclease-free water
tion mixture 10 µL nuclease-free water 18 µL 10X Buffer O 2 µL PagI 1-2 µL BGORTango2X Tango NR NR NR NR – buffer is not recommended, because of high star activity Dam: may overlap – cleavage impaired. Dcm: never overlaps – no effect. CpG: never overlaps – no effect. EcoKI: never overlaps – no effect. EcoBI: may overlap – cleavage impaired. complete digestion of 1 µg ofDigestion of Agarose-e

3 mbedded DNA A minimum of 5 units of the
mbedded DNA A minimum of 5 units of the enzyme is required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours. pBR322 pUC57 pUC18/19 pTZ19R/U M13mp18/19 8 3 4 3 3 2 1 methylation, use a CERTIFICATE OF ANALYSIS Overdigestion Assay PagI (10 U/µg lambda DNA x 16 hours). The ligation and recleavage assay was replaced with LO to trace nuclease and phosphatase activities wit

4 h No detectable degradation of single-st
h No detectable degradation of single-stranded or double-stranded labeled oligonucleotides occurred during incubation with 10 units of PagI for 4 hours. PRODUCT USE LIMITATION The product was not tested for use in diagnostics or for drug Please refer to www.thermoscientific.com/onebio © 2012 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the ific Inc. and its subsidiarie