Environmental health amp Safety Section 2 agenda Section 2 Laboratory Safety Animal Safety Radiation Safety Biosafety Exposure incidents Laboratory Safety Training Requirements Laboratory Safety ID: 779996
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Slide1
New employee safety trainingEnvironmental health & SafetySection 2
Slide2agendaSection 2
Laboratory Safety Animal Safety
Radiation SafetyBiosafetyExposure incidentsLaboratory Safety Training Requirements
Slide3Laboratory SafetyTypes of Hazards
Identifying Chemical HazardsRight to Know/ Globally Harmonized System
Protective MeasuresHazardous WasteSpill ProcedureAir Monitoring
Slide4Laboratory HazardsPhysical hazards
Compressed gasesExplosive materials
Flammable liquids or solidsOrganic peroxidesOxidizersPyrophoric materials (ignites spontaneously in air)Unstable materials (reactive)
Water reactive materials
Health hazards
Carcinogens
Toxic or highly toxic agents
Reproductive toxinsIrritants (cause irreversible inflammatory effect on living tissue)Sensitizers
(allergic reactions in normal tissue after repeated exposure)
Slide5Recognizing Hazards
Slide6Labeling of chemicals (GHS)All containers must be labeled
Regard unlabeled containers as hazardousDo not remove/deface labels
Manufacturers labels need to have:Identity of chemicalName and address of manufacturerAppropriate hazard warning Under the new GHS containers additionally need:Signal Words
Hazard Statements
Precautionary Statements
Pictograms
Laboratory Solutions:
Need to be labeled with the chemical names of all the constituents Do NOT use chemical formulas when labeling solutions
Slide7Safety Data sheets
Available for every hazardous chemical or substanceDetailed information sheet prepared by manufacturer or importer
Provides information on:A material’s chemical make-up and propertiesThe level of protective gear you need to wear to work safely with the materialThe preplanning needed for safely handling spills, fires, and day-to-day operations
How to respond to accidents
Electronic access to SDSs is available on the electronic chemical environmental management site (CEMS). No username/password is required to view SDS.
Slide8Protective measures
Product Substitution
Engineering Controls Safe Work Practices PPE
Slide9Product SubstitutionWhat are the substitutes for hazardous chemicals?
Mercury containing chemicals & instrumentsMercury free bleach and assay kitsUse alcohol thermometers
Ethidium Bromide/ Acrylamide GelsSYBR® SafePrecast Acrylamide GelsSodium AzideKathon CG/ICP PreservativesFormaldehyde/ Formalin Ordering smaller quantities of higher hazard chemicals
Slide10Engineering controlsThe primary means of protection
Chemical Fume Hood
Biosafey Cabinet
Centrifuge Safety Cups
Hand washing Facility
Safer medical devices
Some engineering controls require annual recertification
(e.g. BSCs, VBSEs, fume hoods)
Shielding
Slide11Safe Work PracticesFollow standard operating procedures
Understand hazards of the materials you are working withConsult PI prior to scaling up any experiments
Do not leave laboratory procedures unattendedPractice good housekeepingStore incompatibles separatelyMark peroxidizable chemicals with received and opened dates and dispose of prior to expiration dateAvoid keeping “legacy” and old containers
Texas Tech Lab Explosion
Watch Video!
Please click on the link to view this video
Slide12Personal Protective Equipment Additional means of protection
Latex (bio only) or Nitrile (bio or chemical) exam gloves (double gloves recommended)Safety glasses with side shields/ goggles/face shieldsRespiratory protection
Lab coats, uniformsTyvek gownsSleeves
Watch Video!
Please click on the link to view this video
Slide13Hazardous Waste ProcedureAll waste is collected in the lab’s Satellite Accumulation Area (SAA)
Slide14Chemical Spill procedureContact Operational Engineers at 508-856-3292
Secure the area
Alert othersIf injured, go to Employee Health or Emergency Room
Slide15Air MonitoringIn the occurrence of a chemical spill, EH&S may monitor chemical concentrations in the air
Drager Tubes
4 gas meter
Ammonia Meters
VOC Monitor
Slide16Animal Safety
PPE Handling & Husbandry
ZoonosesAllergiesAnesthetic GasesControlled Substances
Euthanasia
Slide17Personal Protective Equipment (continued)
Scrub suits/ jump suitsSafety glasses/ face shield
Mask/respiratorsHair coversShoe coversGloves
Slide18Husbandry and HandlingHusbandry is the process of providing care for an animalExamples: providing food, shelter, water
Handle animals in manner prescribed for the species to prevent the following:Splash hazardsContact with bodily fluidsBites/Scratches
Slide19Zoonoses & AllergiesZoonoses are diseases that can be transmitted from animals to humansCausative agents include:
Bacteria Viruses (Herpes B Virus)Parasites (Toxoplasma)Fungi (Ringworm) Allergies are immune responses by the body to a substance
Some people develop a respiratory allergy from working with animalsUsually develop within the first 3 yearsMost common allergy is rhinotracheitis
Slide20Anesthetic GasesWaste gases should be properly scavenged If working outside anesthetic machine use a chemical fume hood or Biological Safety Cabinet if it is 100% exhaust
Don’t use Ether Vaporizer should be calibrated annually on machines that use gas anesthetics
Slide21Controlled Substances
In order to work with controlled substances, a researcher must have (or be listed as a registered user) on a controlled substance Researcher Registration
DEA regulates V Schedules of controlled substanceSchedule I: most addictive and abusedi.e. Heroine, MarijuanaSchedule V: not often abused, minimal/no addictive propertiesDPH regulates all prescriptions drugs as schedule VI
Commonly used Controlled Substances
Ketamine (III), Buprenorphine (III)
Controlled substances must be kept in a substantially constructed cabinet, and perpetual inventories of drug usage must be maintained
Slide22EuthanasiaThe intentional causing of death to an animalPainless
Humane & Respectful “Double Kill”The method of euthanasia will be dependent on species in question and should be performed by trained personnel
AvoidSelf injectionExposure to gasesExposure to Bodily Fluids
Slide23Ionizing radiation passes through matter and can cause some of its atoms to become electrically charged, or ionized. In living tissues, the ions caused by such radiation can affect normal biological processesIonizing radiation comes in several different forms:
Alpha particles - are positively charged particles. They are easily stopped by paper or skin, and are only hazardous if alpha-emitting materials are swallowed or breathed into the body.
Beta particles - are electrons and have a greater penetrating power than alpha particles, but can be stopped by thin layers of water, glass or metal. However, beta emitting material can be hazardous if taken into the bodyGamma and X rays - are electromagnetic radiations. They are very penetrating
and heavy shielding materials like lead
and concrete are needed to stop them
Radiation Safety
Additional training through Radiation Safety is required for work with radioactive isotopes
Slide24Biosafety UMMS Exposure Control Plan
[http://inside.umassmed.edu/ehs/manuals/]
Key Components Guidelines for handling biohazardous agents Emergency/ First Aid Procedures Hepatitis B Vaccination Program Engineering Controls Universal Precautions
Work Practice Controls
Personal Protective Equipment (PPE)
Slide25Biohazard Warning Labels
Warning labels required on:Laboratory doorsContainers of regulated wasteRefrigerators and freezers containing blood and other potentially infectious materials
Other containers used to store, transport, or ship blood or other potentially infectious materialsRed bags or containers may be substituted for labels
Slide26OSHA Bloodborne Pathogen Standard
General Industry Standard 29 CFR 1910.1030Purpose: Eliminate or minimize employee occupational
exposure to human-sourced materialsScope:Covers employees who could be “reasonably anticipated” to come into contact with human blood and OPIM (other potentially infectious materials) Does not cover “Good Samaritan” acts
“Universal precautions”
means always treating everyone’s blood and other body fluids as infectious. This precaution is governed by the Occupational Safety and Health Administration
Slide27Bloodborne PathogensMicroorganisms such as viruses or bacteria that are carried in blood and can cause disease in people
Viruses and related diseases specifically addressed by the OSHA Bloodborne Pathogen Standard include:Hepatitis B Virus (HBV)
Hepatitis Hepatitis C Virus (HCV) HepatitisHuman Immunodeficiency Virus (HIV)
Acquired Immunodeficiency
Syndrome (AIDS)
Slide28Transmission
Bloodborne pathogens can be transmitted through contact with infected human or animal blood, unfixed cells, tissues, or organs, or other potentially infectious materials (OPIM)
Hazardous OPIMCerebrospinal fluidSemenVaginal SecretionsSynovial fluid
Pleural fluid
Pericardial fluid
Human tissues, organs, cells
Body fluids of UNKNOWN
origin should be suspectNonhazardous with Casual ContactTears
Sweat
Urine
Feces
Nasal Secretions
Exception:
Human bite transmission of HBV with saliva to blood [OR] if blood is present
Slide29Disease Prevention
Prophylactic treatment (as available and appropriate; UMMS will provide free of charge)HBV vaccineAnti viral / bacterial drugs administered following an exposure
Slide30State of Massachusetts Categories of Biohazardous waste
Human blood, blood products, unfixed cells, tissues, organs, and contaminated itemsContaminated sharps
Cultures & stocks of infectious agentsHuman pathological waste Animal carcasses and/or bedding from animals contaminated with ‘pathogens’
Slide31Laboratory Waste Disposal Simplified
Slide32Biological Agents Registration PrograMPrincipal Investigators required to register with the UMMS Institutional Biosafety Committee (IBC) all research projects that involve the use of any of the following:
Infectious Bacteria and VirusesRecombinant DNA
Microbial toxins (LD50 <100 mg/kg body wt.) Human or non-human primate cells, tissues, blood, or blood productsDHHS/USDA ‘Select Agents”http://inside.umassmed.edu/subjects/biosafety/
Slide33Exposure IncidentIf you are exposed to blood or other bodily fluids:
Wash are immediately with soap and running water for 20 minutes
If cut by an item which has blood on it, try to save the item for contamination testingReport any on the job injuries to their supervisorRequired by HR Policy #06.05.13 File accident/incident report with Employee HealthPhone: 508-856-6263Bugs Beeper: Page 77-2847 (77-Bugs)Seek immediate attention
Employee Health
Emergency Room
Slide34Laboratory Safety TrainingRequired:
New Employee Orientation-Lab Safety TrainingAnnual Lab Safety Training (Online)
Department Specific trainingJob Dependent: Shipping of Biological & Infectious MaterialShipping of Dangerous & Hazardous Substances (Chemical)Radiation Safety Training
Slide35Annual Health & Safety Training (Online)
User will receive “invitation” email to complete online Health & Safety trainingComplete training within 30 days of receiving emailClick on link and set up password
Questions: Contact EH&S at 508-856-3985
Slide36If you have any questions, please contact the EH&S Dept. at 508-856-3985
Thank you!