HiMedia Laboratories - PDF document

HiMedia Laboratories Technical Data Please refer disclaimer Overleaf. Specimen Collection and HandlingFor clinical samples. After use, contaminated materials must be sterilized by autoclaving before discarding. In Vitro diagnostic use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standardprecautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be 2.Certain gram-positiue bontaminants 'e.g. Rtreptobobbi ( and gram-negatiue aabilli may grov on the medium.3.Certain Raprophytes may also grov on the medium . Performace of the medium is expected when used as per the direction on the label within the expiry period when stored at Organism Growth Growth(FD053) ColonyCharacteristic Mycobacterium avium ATCC luxuriant good-luxuriant smooth, non- Mycobacterium gordonae luxuriant good-luxuriant smooth, yellow, Mycobacterium kansasii luxuriant good-luxuriant photochromogenic, Mycobacterium smegmatis luxuriant good-luxuriant wrinkled,creamy M. tuberculosis H37RV luxuriant good-luxuriant granular, rough, Storage and Shelf Life Please refer disclaimer Overleaf. M162 Intended Use: Composition** Ingredients Gms / Litre L-Asparagine 3.600 Monopotassium phosphate 2.400 Magnesium sulphate 0.240 Magnesium citrate 0.600 Potato starch, soluble 30.000 Malachite green 0.400 **Formula adjusted, standardized to suit performance parametersDirections Suspend 37.24 grams in 600 ml purieied . distilled water containing 12 ml glycerol (for bovine bacteria or other glycerophobic organismsadditions of glycerol is not desirable). Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbspressure (121C) for 15 minutes.Meanwhile prepare 1000 ml of whole egg emulsion collected aseptically. Aseptically add andmix egg emulsion base and Gruft Mycobacterial Supplement (FD053) (if desired) gently to obtain uniform mixture. Distributein sterile screw capped tubes. Arrange tubes in a slanted position. Coagulate and Principle And Interpretation Solid media used for isolation and cultivation of Mycobacteria are either egg-based or agar-based. Egg-based media containwhole eggs or egg yolk, potato flour, salts and glycerol and are solidified by inspissation. Of the egg-based media,LowensteinJensen Medium is most commonly used (1). L.J. Medium was originally formulated by Lowenstein, containingcongo red and malachite green dyes (2). Jensen (3) modified Lowensteins medium by altering the citrate and phosphatecontents, eliminating the congo red dye and by increasing the malachite green concentration. Gruft (4, 5) further modified L.J. Medium with the addition of two antimicrobics to increase selectivity. This medium supports the growth of a wide variety Penicillin and Nalidixic acid (FD053) along with malachite green prevents growth of the majority of contaminants survivingdecontamination of the specimen while encouraging earliest possible growth of. RNA (FD053) acts asstimulant and help to increase the isolation rate of . Do not add glycerol to the medium if bovine or otherglycerophobic strains are to be cultured (7). Malachite green serves as an inhibitor and also as pH indicator. Formation of zone indicates a decrease in pH by gram-positive contaminants (e.g. ) and yellow zones of dye destruction gram-negative bacilli. Proteolytic contaminants cause localized or complete digestion of medium. Hardy et al (8) b.To identify presence or absence of pigmentation by photochromogenes and scotochromogenes at 35°C alternately in light and dark as per the type of organism.Routinely cultivation is carried out aerobically at 35°C. Type of specimen HiMedia Laboratories Technical Data User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Followestablished laboratory procedures in disposing of infectious materials and material that comes into contact with clinicalsample must be decontaminated and disposed of in accordance with current laboratory techniques ().Reference 1. Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H., (Eds.), 8th Ed., 2003, Manual of Clinical . Jensen K. A., 1932, Zentralb. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. I Orig., 125:222. . Forbes B. A., Sahm A. S. and Weissfeld D. F., Bailey & Scotts Diagnostic Microbiology, 10th Ed., 1998, Mosby, Inc., St. Revision : 0 / 201 . Gruft, 1971, Health Lab. Sci., 8:79. . Gruft, 1963, Am. Rev. Respir. Dis., 88:412. . Isenberg, (Ed.), Clinical Microbiology Procedures Handbook . Jorgensen,J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L.,Richter, S.S and Warnock., D.W. (2015) Manualof Clinical Microbiology, 11th Edition. Vol. 1. . Lowenstein E., 1931, Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig., 120:127. . MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, WilliamsIn vitro diagnostic medicalnot use if package is ,Esdoornlaan 13, 3951 IVD 34 Vaeiaoi Ioeutusiam Etuaue- Utes nutu eotuse tuiuabimiuy pg uie pspeudu(t) io uieis appmidauipo psips up ute. Pspeudut dpogpsn tpmemy up uie iogpsnauipo dpouaioee iouiit aoe puies semauee HiMeeia™ pubmidauipot. Tie iogpsnauipo dpouaioee io uiit pubmidauipo it batee po pus seteasdi aoe eevemppneouwpsl aoe it up uie betu pg pus lopwmeehe usue aoe addusaue. HiMeeia™ Labpsaupsiet Pvu Lue setesvet uie sihiu up nale diaohet uptpedigidauipot aoe iogpsnauipo semauee up uie pspeudut au aoy uine. Pspeudut ase opu ioueoeee gps iunao ps aoinam ps uiesapeuuid ute buugps mabpsaupsy-eiahoptuid- seteasdi ps gusuies naougaduusioh ute pomy- uomett puieswite tpedigiee. Suaueneout dpouaioee ieseio tipume opu HiMedia Laboratories Technical Data User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Followestablished laboratory procedures in disposing of infectious materials and material that comes into contact with clinicalsample must be decontaminated and disposed of in accordance with current laboratory techniques ().Reference MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, . Jensen K. A., 1932, Zentralb. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. I Orig., 125:222. . Forbes B. A., Sahm A. S. and Weissfeld D. F., Bailey & Scotts Diagnostic Microbiology, 10th Ed., 1998, Louis, Mo. Revision : 0 / 201 . Gruft, 1971, Health Lab. Sci., 8:79. . Gruft, 1963, Am. Rev. Respir. Dis., 88:412. . Isenberg, (Ed.), Clinical Microbiology Procedures Handbook . Jorgensen,J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L.,Richter, S.S and Warnock., D.W. (2015) Manual In vitro diagnostic medicaluse if package is ,Esdoornlaan 13, 3951 IVD 34 Vaeiaoi Ioeutusiam Etuaue- Utes nutu eotuse tuiuabimiuy pg uie pspeudu(t) io uieis appmidauipo psips up ute. Pspeudut dpogpsn tpmemy up uie iogpsnauipo dpouaioee iouiit aoe puies semauee HiMeeia™ pubmidauipot. Tie iogpsnauipo dpouaioee io uiit pubmidauipo it batee po pus seteasdi aoe eevemppneouwpsl aoe it up uie betu pg pus lopwmeehe usue aoe addusaue. HiMeeia™ Labpsaupsiet Pvu Lue setesvet uie sihiu up nale diaohet uptpedigidauipot aoe iogpsnauipo semauee up uie pspeudut au aoy uine. Pspeudut ase opu ioueoeee gps iunao ps aoinam ps uiesapeuuid ute buugps mabpsaupsy-eiahoptuid- seteasdi ps gusuies naougaduusioh ute pomy- uomett puieswite tpedigiee. Suaueneout dpouaioee ieseio tipume opu HiMedia Laboratories Technical Data User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Followestablished laboratory procedures in disposing of infectious materials and material that comes into contact with clinicalsample must be decontaminated and disposed of in accordance with current laboratory techniques ().Reference .Lowenstein E., 1931, Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig., 120:127.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, . Jensen K. A., 1932, Zentralb. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. I Orig., 125:222. . Forbes B. A., Sahm A. S. and Weissfeld D. F., Bailey & Scotts Diagnostic Microbiology, 10th Ed., 1998, Mosby, Inc., Louis, Mo. Revision : 0 / 201 . Gruft, 1971, Health Lab. Sci., 8:79. . Gruft, 1963, Am. Rev. Respir. Dis., 88:412. . Isenberg, (Ed.), Clinical Microbiology Procedures Handbook . Jorgensen,J.H., Pfaller, M.A., Carroll, K.C., Funke, G.,

Landry, M.L.,Richter, S.S and Warnock., D.W. (2015) Manual In vitro diagnostic medical,Esdoornlaan 13, 3951 IVD 34 Vaeiaoi Ioeutusiam Etuaue- Utes nutu eotuse tuiuabimiuy pg uie pspeudu(t) io uieis appmidauipo psips up ute. Pspeudut dpogpsn tpmemy up uie iogpsnauipo dpouaioee iouiit aoe puies semauee HiMeeia™ pubmidauipot. Tie iogpsnauipo dpouaioee io uiit pubmidauipo it batee po pus seteasdi aoe eevemppneouwpsl aoe it up uie betu pg pus lopwmeehe usue aoe addusaue. HiMeeia™ Labpsaupsiet Pvu Lue setesvet uie sihiu up nale diaohet uptpedigidauipot aoe iogpsnauipo semauee up uie pspeudut au aoy uine. Pspeudut ase opu ioueoeee gps iunao ps aoinam ps uiesapeuuid ute buugps mabpsaupsy-eiahoptuid- seteasdi ps gusuies naougaduusioh ute pomy- uomett puieswite tpedigiee. Suaueneout dpouaioee ieseio tipume opu Please refer disclaimer Overleaf. M162 Intended Use: Composition** Ingredients Gms / Litre L-Asparagine 3.600 Monopotassium phosphate 2.400 Magnesium sulphate 0.240 Magnesium citrate 0.600 Potato starch, soluble 30.000 Malachite green 0.400 **Formula adjusted, standardized to suit performance parametersDirections Suspend 37.24 grams in 600 ml purieied . distilled water containing 12 ml glycerol (for bovine bacteria or other glycerophobic organismsadditions of glycerol is not desirable). Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbspressure (121C) for 15 minutes.Meanwhile prepare 1000 ml of whole egg emulsion collected aseptically. Aseptically add andmix egg emulsion base and Gruft Mycobacterial Supplement (FD053) (if desired) gently to obtain uniform mixture. Distributein sterile screw capped tubes. Arrange tubes in a slanted position. Coagulate and Principle And Interpretation Solid media used for isolation and cultivation of Mycobacteria are either egg-based or agar-based. Egg-based media containwhole eggs or egg yolk, potato flour, salts and glycerol and are solidified by inspissation. Of the egg-based media,LowensteinJensen Medium is most commonly used (1). L.J. Medium was originally formulated by Lowenstein, containingcongo red and malachite green dyes (2). Jensen (3) modified Lowensteins medium by altering the citrate and phosphateeliminating the congo red dye and by increasing the malachite green concentration. Gruft (4, 5) further modified L.J. Medium with the addition of two antimicrobics to increase selectivity. This medium supports the growth of a wide variety Penicillin and Nalidixic acid (FD053) along with malachite green prevents growth of the majority of contaminants survivingdecontamination of the specimen while encouraging earliest possible growth of. RNA (FD053) acts asstimulant and help to increase the isolation rate of . Do not add glycerol to the medium if bovine or otherglycerophobic strains are to be cultured (7). Malachite green serves as an inhibitor and also as pH indicator. Formation of zone indicates a decrease in pH by gram-positive contaminants (e.g. ) and yellow zones of dye destruction gram-negative bacilli. Proteolytic contaminants cause localized or complete digestion of medium. Hardy et al ( b.To identify presence or absence of pigmentation by photochromogenes and scotochromogenes at 35°C alternately in light and dark as per the type of organism.Routinely cultivation is carried out aerobically at 35°C. Type of specimen HiMedia Laboratories Technical Data Please refer disclaimer Overleaf. Specimen Collection and HandlingFor clinical samples. After use, contaminated materials must be sterilized by autoclaving before discarding. In Vitro diagnostic use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standardprecautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be 2.Certain gram-positiue bontaminants 'e.g. Rtreptobobbi ( and gram-negatiue aabilli may grov on the medium.3.Certain Raprophytes may also grov on the medium . Performace of the medium is expected when used as per the direction on the label within the expiry period when stored at Organism Growth Growth(FD053) ColonyCharacteristic Mycobacterium avium ATCC luxuriant good-luxuriant smooth, non- Mycobacterium gordonae luxuriant good-luxuriant smooth, yellow, Mycobacterium kansasii luxuriant good-luxuriant photochromogenic, Mycobacterium smegmatis luxuriant good-luxuriant wrinkled,creamy M. tuberculosis H37RV luxuriant good-luxuriant granular, rough, Storage and Shelf Life Please refer disclaimer Overleaf. M162 Intended Use: Composition** Ingredients Gms / Litre L-Asparagine 3.600 Monopotassium phosphate 2.400 Magnesium sulphate 0.240 Magnesium citrate 0.600 Potato starch, soluble 30.000 Malachite green 0.400 **Formula adjusted, standardized to suit performance parametersDirections Suspend 37.24 grams in 600 ml purieied . distilled water containing 12 ml glycerol (for bovine bacteria or other glycerophobic organismsadditions of glycerol is not desirable). Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbspressure (121C) for 15 minutes.Meanwhile prepare 1000 ml of whole egg emulsion collected aseptically. Aseptically add andmix egg emulsion base and Gruft Mycobacterial Supplement (FD053) (if desired) gently to obtain uniform mixture. Distributein sterile screw capped tubes. Arrange tubes in a slanted position. Coagulate and Principle And Interpretation Solid media used for isolation and cultivation of Mycobacteria are either egg-based or agar-based. Egg-based media containwhole eggs or egg yolk, potato flour, salts and glycerol and are solidified by inspissation. Of the egg-based media,LowensteinJensen Medium is most commonly used (). L.J. Medium was originally formulated by Lowenstein, containingcongo red and malachite green dyes (). Jensen () modified Lowensteins medium by altering the citrate and phosphatecontents, eliminating the congo red dye and by increasing the malachite green concentration. Gruft () further modified L.J. Medium with the addition of two antimicrobics to increase selectivity. This medium supports the growth of a wide variety Penicillin and Nalidixic acid (FD053) along with malachite green prevents growth of the majority of contaminants survivingdecontamination of the specimen while encouraging earliest possible growth of. RNA (FD053) acts asstimulant and help to increase the isolation rate of . Do not add glycerol to the medium if bovine or otherglycerophobic strains are to be cultured (). Malachite green serves as an inhibitor and also as pH indicator. Formation of zone indicates a decrease in pH by gram-positive contaminants (e.g. ) and yellow zones of dye destruction gram-negative bacilli. Proteolytic contaminants cause localized or complete digestion of medium. Hardy et al ( b.To identify presence or absence of pigmentation by photochromogenes and scotochromogenes at 35°C alternately in light and dark as per the type of organism.Routinely cultivation is carried out aerobically at 35°C. Type of specimen HiMedia Laboratories Technical Data User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Followestablished laboratory procedures in disposing of infectious materials and material that comes into contact with clinicalsample must be decontaminated and disposed of in accordance with current laboratory techniques (). Reference .Lowenstein E., 1931, Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig., 120:127.of Medical Bacteria, Vol. 1, . Jensen K. A., 1932, Zentralb. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. I Orig., 125:222. . Forbes B. A., Sahm A. S. and Weissfeld D. F., Bailey & Scotts Diagnostic Microbiology, 10th Ed., 1998, Mosby, Inc., Louis, Mo. Revision : 0 / 201 . Gruft, 1971, Health Lab. Sci., 8:79. . Gruft, 1963, Am. Rev. Respir. Dis., 88:412. . Isenberg, (Ed.), Clinical Microbiology Procedures Handbook . Jorgensen,J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L.,Richter, S.S and Warnock., D.W. (2015) Manual In vitro diagnostic medical,Esdoornlaan 13, 3951 IVD 34 Vaeiaoi Ioeutusiam Etuaue- Utes nutu eotuse tuiuabimiuy pg uie pspeudu(t) io uieis appmidauipo psips up ute. Pspeudut dpogpsn tpmemy up uie iogpsnauipo dpouaioee iouiit aoe puies semauee HiMeeia™ pubmidauipot. Tie iogpsnauipo dpouaioee io uiit pubmidauipo it batee po pus seteasdi aoe eevemppneouwpsl aoe it up uie betu pg pus lopwmeehe usue aoe addusaue. HiMeeia™ Labpsaupsiet Pvu Lue setesvet uie sihiu up nale diaohet uptpedigidauipot aoe iogps

nauipo semauee up uie pspeudut au aoy uine. Pspeudut ase opu ioueoeee gps iunao ps aoinam ps uiesapeuuid ute buugps mabpsaupsy-eiahoptuid- seteasdi ps gusuies naougaduusioh ute pomy- uomett puieswite tpedigiee. Suaueneout dpouaioee ieseio tipume opu HiMedia Laboratories Technical Data Please refer disclaimer Overleaf. Specimen Collection and HandlingFor clinical samples. After use, contaminated materials must be sterilized by autoclaving before discarding. In Vitro diagnostic use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standardprecautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be 2.Certain gram-positiue bontaminants 'e.g. Rtreptobobbi ( and gram-negatiue aabilli may grov on the medium.3.Certain Raprophytes may also grov on the medium . Performace of the medium is expected when used as per the direction on the label within the expiry period when stored at Organism Growth Growthwith GruftSupplement(FD053) ColonyCharacteristic Mycobacterium avium ATCC25291 luxuriant good-luxuriant smooth, non-colonies Mycobacterium gordonaeATCC 14470 luxuriant good-luxuriant smooth, yellow, Mycobacterium kansasiiATCC 12478 luxuriant good-luxuriant photochromogenic,rough Mycobacterium smegmatis luxuriant good-luxuriant wrinkled,creamy M. tuberculosis H37RV luxuriant good-luxuriant granular, rough,friable colonies Storage and Shelf Life Please refer disclaimer Overleaf. M162 Intended Use: Composition** Ingredients Gms / Litre L-Asparagine 3.600 2.400 Magnesium sulphate 0.240 Magnesium citrate 0.600 Potato starch, soluble 30.000 Malachite green 0.400 **Formula adjusted, standardized to suit performance parametersDirections Suspend 37.24 grams in 600 ml purieied . distilled water containing 12 ml glycerol (for bovine bacteria or other glycerophobic organismsadditions of glycerol is not desirable). Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbspressure (121C) for 15 minutes.Meanwhile prepare 1000 ml of whole egg emulsion collected aseptically. Aseptically add andmix egg emulsion base and Gruft Mycobacterial Supplement (FD053) (if desired) gently to obtain uniform mixture. Distributein sterile screw capped tubes. Arrange tubes in a slanted position. Coagulate and Principle And Interpretation Solid media used for isolation and cultivation of Mycobacteria are either egg-based or agar-based. Egg-based media containwhole eggs or egg yolk, potato flour, salts and glycerol and are solidified by inspissation. Of the egg-based media,LowensteinJensen Medium is most commonly used (). L.J. Medium was originally formulated by Lowenstein, containingcongo red and malachite green dyes (). Jensen () modified Lowensteins medium by altering the citrate and phosphateeliminating the congo red dye and by increasing the malachite green concentration. Gruft () further modified L.J. Medium with the addition of two antimicrobics to increase selectivity. This medium supports the growth of a wide variety Penicillin and Nalidixic acid (FD053) along with malachite green prevents growth of the majority of contaminants survivingdecontamination of the specimen while encouraging earliest possible growth of. RNA (FD053) acts asstimulant and help to increase the isolation rate of . Do not add glycerol to the medium if bovine or otherglycerophobic strains are to be cultured (). Malachite green serves as an inhibitor and also as pH indicator. Formation of zone indicates a decrease in pH by gram-positive contaminants (e.g. ) and yellow zones of dye destruction bacilli. Proteolytic contaminants cause localized or complete digestion of medium. Hardy et al ( b.To identify presence or absence of pigmentation by photochromogenes and scotochromogenes at 35°C alternately in light and dark as per the type of organism.Routinely cultivation is carried out aerobically at 35°C. Type of specimen Please refer disclaimer Overleaf. M162 Intended Use: Composition** Ingredients Gms / Litre L-Asparagine 3.600 2.400 Magnesium sulphate 0.240 Magnesium citrate 0.600 Potato starch, soluble 30.000 Malachite green 0.400 **Formula adjusted, standardized to suit performance parametersDirections Suspend 37.24 grams in 600 ml purieied . distilled water containing 12 ml glycerol (for bovine bacteria or other glycerophobic organismsadditions of glycerol is not desirable). Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbspressure (121C) for 15 minutes. Cool to 45-50°C.Meanwhile prepare 1000 ml of whole egg emulsion collected aseptically. Aseptically add andmix egg emulsion base and Gruft Mycobacterial Supplement (FD053) (if desired) gently to obtain uniform mixture. Distributein sterile screw capped tubes. Arrange tubes in a slanted Principle And Interpretation Solid media used for isolation and cultivation of Mycobacteria are either egg-based or agar-based. Egg-based media containwhole eggs or egg yolk, potato flour, salts and glycerol and are solidified by inspissation. Of the egg-based media,LowensteinJensen Medium is most commonly used (). L.J. Medium was originally formulated by Lowenstein, containingcongo red and malachite green dyes (). Jensen () modified Lowensteins medium by altering the citrate and phosphatecontents, eliminating the congo red dye and by increasing the malachite green concentration. Gruft () further modified L.J. Medium with the addition of two antimicrobics to increase selectivity. This medium supports the growth of a wide variety Penicillin and Nalidixic acid (FD053) along with malachite green prevents growth of the majority of contaminants survivingdecontamination of the specimen while encouraging earliest possible growth of. RNA (FD053) acts asstimulant and help to increase the isolation rate of . Do not add glycerol to the medium if bovine or otherglycerophobic strains are to be cultured (). Malachite green serves as an inhibitor and also as pH indicator. Formation of indicates a decrease in pH by gram-positive contaminants (e.g. ) and yellow zones of dye destruction gram-negative bacilli. Proteolytic contaminants cause localized or complete digestion of medium. Hardy et al ( b.To identify presence or absence of pigmentation by photochromogenes and scotochromogenes at 35°C alternately in light and dark as per the type of organism.Routinely cultivation is carried out aerobically at 35°C. Type of specimen HiMedia Laboratories Technical Data User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Followestablished laboratory procedures in disposing of infectious materials and material that comes into contact with clinicalsample must be decontaminated and disposed of in accordance with current laboratory techniques (). Reference .Lowenstein E., 1931, Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig., 120:127.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, . Jensen K. A., 1932, Zentralb. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. I Orig., 125:222. . Forbes B. A., Sahm A. S. and Weissfeld D. F., Bailey & Scotts Diagnostic Microbiology, 10th Ed., 1998, Mosby, Inc., Louis, Mo. Revision : 0 / 201 . Gruft, 1971, Health Lab. Sci., 8:79. . Gruft, 1963, Am. Rev. Respir. Dis., 88:412. . Isenberg, (Ed.), Clinical Microbiology Procedures Handbook . Jorgensen,J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L.,Richter, S.S and Warnock., D.W. (2015) Manual In vitro diagnostic medical IVD 34 Vaeiaoi Ioeutusiam Etuaue- Utes nutu eotuse tuiuabimiuy pg uie pspeudu(t) io uieis appmidauipo psips up ute. Pspeudut dpogpsn tpmemy up uie iogpsnauipo dpouaioee iouiit aoe puies semauee HiMeeia™ pubmidauipot. Tie iogpsnauipo dpouaioee io uiit pubmidauipo it batee po pus seteasdi aoe eevemppneouwpsl aoe it up uie betu pg pus lopwmeehe usue aoe addusaue. HiMeeia™ Labpsaupsiet Pvu Lue setesvet uie sihiu up nale diaohet uptpedigidauipot aoe iogpsnauipo semauee up uie pspeudut au aoy uine. Pspeudut ase opu ioueoeee gps iunao ps aoinam ps uiesapeuuid ute buugps mabpsaupsy-eiahoptuid- seteasdi ps gusuies naougaduusioh ute pomy- uomett puieswite tpedigiee. Suaueneout dpouaioee ieseio tipume opu HiMeeia Labpsaupsiet Pvu. Lue. Reh.pggide : 34- Vaeiaoi Ioe.Etu.- LCS Mash- Munbai.511197- Ioeia Dutupnes dase Np.: 133.7227 9898pggide : A.627-Swatuil Eitia Cutioett Pasl-Via Vaeiaoi Ioe. Etu.- LCS Mash- Munbai.511197- Ioeia. Dutupnes dase Np.: 133.7258 2929 Enai