TH TH H E R E R EAL EAL O ON N E S E S T EP R Pathogen and product description species are gramnegative nonspore forming spiral or curvedshaped bacteria Among the more than 26 species currentl ID: 843384
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1 TH TH H E R E R EAL EAL O ON N E S E S T
TH TH H E R E R EAL EAL O ON N E S E S T EP R Campylobacter coli, C. lari & C. jejuni Real Time PCR Detection Pathogen and product description species are gram-negative, nonspore forming, spiral, or curved-shaped bacteria. Among the more than 26 species currently classified in the genus, most human diseases are attributed to three major food-borne species: Campylobacter . These three species can be isolated from poultry and are of greatest concern to the poultry industry. is considered one of the most common causes of diarrheal illness worldwide. Poultry is a major reservoir and source of transmission of Campylobacter is the common is often isolated from is predominant in shorebirds. Other risk factors include consumption of animal products C a a m m p y y l l o o b b a a cter co l l i i C. e e j j u u n n i R e e a a l l T T i m m e e PCR D D e e t e c t i o n Ki t Work Flow Components ReferenceDescriptionVIASURE Campylobacter coli, C. lari & C. jejuni Real Time PCR Detection Kit 6 x 8-well strips, low profileVIASURE Campylobacter coli, C. lari & C. jejuni Real Time PCR Detection Kit 6 x 8-well strips, high profileVIASURE Campylobacter coli, C. lari & C. jejuni Real Time PCR Detection Kit 12 x 8-well strips, low profileVIASURE Campylobacter coli, C. lari & C. jejuni Real Time PCR Detection Kit 12 x 8-well strips, high profileVIASURE Campylobacter coli, C. lari & C.
2 jejuni Real Time PCR Detection Kit 96-we
jejuni Real Time PCR Detection Kit 96-well plate, low profileVIASURE Campylobacter coli, C. lari & C. jejuni Real Time PCR Detection Kit 96-well plate, high profileCERTEST BIOTEC, S.L.Pol. Industrial Río Gállego II, Calle J, Nº 1,50840, San Mateo de Gállego, Zaragoza (SPAIN)www.certest.es STEP 1Add 15 µl ofrehydration bufferinto each wellwells and add yourextracted DNASTEP 3Load the strips into thethermocycler and runthe specified protocol STEP 2Add 5 µl of DNA sample /positive control /negative control STEP 4Interpretate results Figure 1. CFX96 Real-Time PCR Detection System(channel FAM).Figure 2.CFX96 Real-Time PCR Detection SystemFigure 3.CFX96 Real-Time PCR Detection System Real Time PCR Detection Kit has a detection limit of 10 DNA copies per ,i>Ã/iÃ* ,ÃiÃiVÃÃÃÃÃUÃ6iÃÃ"\à £Ã£n Reagent/MaterialDescriptionColourQuantityCampylobacter coli, C. lari & C. jejuniA mix of enzymes, primers-probes, buffer, dNTPs, stabilizers and Internal White6/12 x 8-well Rehydration BufferBlue1 vial x 1,8 mLCampylobacter coli, C. lari & C. jejuni Red1 vialExtraction ControlNon-infectious nucleic acid lyophilizedGreen1 vialNon template controlViolet1 vial x 1 mLWater RNAse/DNAse free RNAse/DNAse free waterWhite1 vial x 1 mLTear-off 8-cap stripsTransparent6/12 x 8-ca