i locity 2019FMPCCUKI02 ViCELL BLU Cell concentration and viability Increase your vi locity ViCELL BLU History of Cell Counting Coulter Model A 1953 Electrical Sensing Zone ID: 1042281
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1. Vi-cell bluIncrease your vi-locity2019-FM-PCC-UKI-02
2. Vi-CELL BLU Cell concentration and viabilityIncrease your vi-locity
3. Vi-CELL BLU History of Cell CountingCoulter Model A 1953Electrical Sensing Zone Principle„Coulter Counting“ZM1982 Z21994Multisizer 4e 2016Image Based Cell AnalyzerVi-CELL XR2003Vi-CELL BLU 2019
4. Vi-CELL BLU IntroductionAdvantages of the Vi-CELL BLU:Faster analysis: 130 secLess volume: 200 µlSOP with fully automated routines incl. cleaning24 position carousel96 well plate loaderWin 10 OS integrated computerTouchscreen interface
5. Vi-CELL BLUTrypan blue methodPrinciple:Live cells with intact cell membranes exclude dyes like trypan blue cells look translucent green circledead cells allow the dye to penetrate through their damaged membranecells look darkred circle
6. Vi-CELL BLUInstrument
7. Vi-CELL BLUInstrument
8. Vi-CELL BLUOptical sensorVi-CELL XRVi-CELL BLUThe larger sensor size of the Vi-CELL BLU and optical configuration results in approximately 1.7 fold greater imaged area resulting in more objects captured per image. Coupled with the faster acquisition speed of the camera it is possible to image 100 frames in less time that required by the Vi-CELL XR. This results in increased statistical confidence in the results.
9. Vi-CELL BLUResolutionWhile the physical size of the images from the Vi-CELL BLU are only 1.7x larger they pack almost twice as many pixels into that space. This higher pixel density produces a much smoother gradation of object edges making for a more accurate segmentation of object boundaries and is particularly advantageous for declustering operations.Vi-CELL BLUVi-CELL XR
10. Vi-CELL BLUInterface
11. Vi-CELL BLUInterface
12. 2.5mL sample vial 0.60 mL sample vial Vi-CELL BLUSample Volume
13. Vi-CELL BLUEasier to Use and Greater Traceability310 activities per kitRFID tag keeps track of:Lot numberExpiration dateOn-board life (90 days)Activities remaining
14. Vi-CELL BLUFlexibility: Changing from carousel to plate
15. Vi-CELL BLUSingle Use ControlsConcentration0,5x10^6 (0,5 M)2x10^6 (2M)4x10^6 (4M)1x10^7 (10M)Viability50% Viability Mixture of small and large beads.Size10 micron NIST size beads Focus monitoring
16. Vi-CELL BLUData IntegrityAudit TrailsError LogSystem Activity LogUser LoginUser LevelsElectronic SignaturesExporting/Backup/Clean-up21 CFR Part 11 Compliance
17. Vi-CELL BLUCell TypeCell typeMinimum threshold for detected cells (lowest 2 µm)Maximum threshold for detected cells (highest 60 µm)Number of images (recommended 100)Defines the sharpness of the separation of the cell from the background. Can be used to reject debris (least circular=0, perfectly circular =1)Increases the ability to detect cells that are clumped together. Default setting is Medium.Number of resuspension cycles. Default setting is 3.Defines the brightness degree from which a cell is defined as a vital cell. 55% is a typical value.Defines the size of the inner bright area defining a vital cell. 5 – 10% are typical valuesNumber of mixing cycles with trypan blue and cell suspension.
18. Vi-CELL BLUDeclusteringRaw picture dataAnnotated data
19. Vi-CELL BLUSpecifications Product is not verified or validated for use in diagnostic procedures. Cu