Summary MetaSUB annual meeting Shanghai China 20160701 Klas I Udekwu Per O Ljungdahl Stockholm Subway system 1057 km in length 385 million passengers per year Ca 1 million pasengers per day ID: 564410
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Stockholm CSD16 Summary
MetaSUB annual meetingShanghai, China 20160701
Klas I. Udekwu
Per O. LjungdahlSlide2
Stockholm Subway system
105.7 km in length385 million passengers per yearCa 1 million pasengers per day
3 lines (Red Blue and Green)Station types Underground (mountainrock), Concreteclad, Open, MixedEthnicity study confounded by laws to ’protect’ minoritiesCircumvented by using minority groupingSocioeconomic statusLow middle to Upper class
Recent change in operator
MTR of Hong Kong won bid to run the system
Can’t compare pilot samples with CSD and continued samplingSlide3
Stockholm Subway systemSlide4
Sampling project_Pilot 1
9 stations chosen 5-7 samples per station10 volunteers in total
Sampling was on turnstiles (card reader)Escalator railingsSeatsTrain seatsTrain holding barsTicket machines (Touchscreens)Slide5
Pilot sampling March 15-17 2016
Number of target stations 8 Traffic status High_Low
Sampling frequency High_Low Optioned Stations 1.T-Centralen (1964, Betong) 2.Brommaplan (1952, Ytstation) 3.Slussen (1950, Betong) 4. Fittja (1972, Ytstation, Röd) contd. 5. Fruängen (1964, Ytstation, Röd) 6.Västra Skogen (1975, Bergstation) 7.Skarpnäck (1994, Bergstation) 8. Danderyds sjukhus (1978, Betong, Röd) 9. Globen (1951, Ytstation, Grön)Slide6
Significant reduction in yield with centrifugation
Pilot sample extraction improved yieldSlide7
Plan for Sampling_June 21 & 22
100 stations# Samples per station3 to 4 samples per stationSame scheme as in pilot: Escalator handrails, touchscreens, turnstiles
# Samples from trains30 to 50 depending on number of trains sampled in depot Other samples of the urban environment in close proximity to stations with hospitals in immediate radius30 to 50
N
t
~ 400 samples June 2016Slide8Slide9
Surface types and sample frequency (n = 404)
CSD16-STO-001 (Stockholm unveiled)Slide10
CSD16-STO-001 (Stockholm unveiled)Slide11
Antibiotic Resistance
Plasmid extraction
gDNA extraction
Transformation & platingSlide12Slide13
Stockholm MetaSUB
Acknowledged: Chris, Sofia, Ebrahim, Maureen, Leming and.........YOU ALL