Cultured cell models Primary cellsAirwayBronchiolar epithelia Fibroblasts Alveolar type II epithelial cells macrophages neutrophilsTumor derived cellsHeLa cells H441 cellsEstablished cell linesC ID: 821093
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CF@lantaExperimental Models Core Airway
CF@lantaExperimental Models Core Airway Epithelial CellsCF & Airways Disease Research WorkshopDecember Cultured cell modelsPrimary cellsAirway/Bronchiolar epithelia, F
ibroblasts, Alveolar type II epithelial
ibroblasts, Alveolar type II epithelial cells, macrophages, neutrophilsTumor derived cellsHeLa cells, H441 cellsEstablished cell linesCFBE41o, 16HBE14o, Calu3, IB3, NuLi
, CuFiHayflick, L. The limited in vitro
, CuFiHayflick, L. The limited in vitro lifetime of human diploidcell strains. Exp. Cell Res. 37, 614636 (1965).Primary cells do not have unlimited proliferative capaci
tybeatricebiologist.com/2010/06/chromos
tybeatricebiologist.com/2010/06/chromosomes.htmlHayflick, his limit, and cellular ageing, Jerry W. Shay and Woodring E.Wright, Nature Reviews Molecular Cell Biology.Tel
omerase therapeutics for cancer: challen
omerase therapeutics for cancer: challenges and new directions, Jerry W. Shay & Woodring E. Wright, Nature Reviews Drug Discovery 5, 577584 (2006)Telomere shortening in
duces cell senescenceZabner, 2003NuLiCu
duces cell senescenceZabner, 2003NuLiCuFicells have a “reasonable chromosome count and represent different CF genotypesNuLiCuFicells stabilize after 6 weeks of di
fferentiationForskolin/IBMX activates CF
fferentiationForskolin/IBMX activates CFTRGlyH, BumentamideCFTR inhibitorsTER (Ohm x cmweeks after airliftTER (Ohm x cmweeks after airliftTER (Ohm x cmweeks after air
liftTER (Ohm x cmcells seeded (x 10TER
liftTER (Ohm x cmcells seeded (x 10TER (Ohm x cmcells seeded (x 10TER (Ohm x cmcells seeded (x 10NuLiCuFicells stabilize after 6 weeks of differentiationNuLiCuFi4 het
G551D/delFCuFidelF/delFCFTR activity in
G551D/delFCuFidelF/delFCFTR activity in F508delCFTR expressing CuFicells is partially restored Forskolin/IBMX activates CFTRGlyH, BumentamideCFTR inhibitorsCuFi5 cells
Fdel508CFTR affects Cx43 but not Cx26Sp
Fdel508CFTR affects Cx43 but not Cx26Specific impairment of Cx43 in F508delCFTR expressing CuFi5 cellsCx43Cx26NuLiCuFiFdel508CFTRwtCFTRNuLi1 cells form spheroid epit
helia1 cells cultured in 1:1 Matrigel:Ul
helia1 cells cultured in 1:1 Matrigel:Ultroserdifferentiation medium, 20 daysLive cell microscopy with the Nikon A1R laser scanning confocal microscopy.Nasal epithelial
cells as a model system that leverages
cells as a model system that leverages the CFW1282X/W1282X F508del/G551DF508del/F508delG551D/R117H“wild type”Nasal epithelial cells from an Fdel508/Fdel508 p