Overview Sample Collection Just In Time Training Diagnostic Sampling Overview Before You Begin Test specifications from laboratory Samples needed any special media Temperature Gather all supplies ID: 1009073
Download Presentation The PPT/PDF document "Diagnostic Sampling Collection, Packagin..." is the property of its rightful owner. Permission is granted to download and print the materials on this web site for personal, non-commercial use only, and to display it on your personal computer provided you do not modify the materials and that you retain all copyright notices contained in the materials. By downloading content from our website, you accept the terms of this agreement.
1. Diagnostic SamplingCollection, Packaging, ShippingOverview
2. Sample CollectionJust In Time TrainingDiagnostic Sampling: Overview
3. Before You BeginTest specifications from laboratorySamples needed, any special media TemperatureGather all suppliesPre-label specimen containersGather PPECoveralls, footwear, glovesRespirators, face shieldsJust In Time TrainingDiagnostic Sampling: Overview
4. LabelingPencil, waterproof inkClear, legibleAbility to trace sampleAnimal, group, or premise identificationSample typeSwab location Tissue or organ Date Just In Time TrainingDiagnostic Sampling: Overview
5. Sample TypesWhole blood or serumBlood tube typeSwabsCulturePCRSkin samplesTissuesFormalinRefrigeratedJust In Time TrainingDiagnostic Sampling: Overview
6. Sample Collection & Handling From individual animals, not groupsPrevent sample degradationSelective or transport mediaIce packs or dry iceFormalinAt least 1:10ratio ofsample:formalinJust In Time TrainingDiagnostic Sampling: Overview
7. Sample Collection & Handling Prevent contamination PPEDisposable glovesDisposable equipmentNeedles, syringes, bladesBiosecurityDisinfectionJust In Time TrainingDiagnostic Sampling: Overview
8. Sample PackagingJust In Time TrainingDiagnostic Sampling: Overview
9. Prior to ShippingSamples must be packaged to withstand:Shocks and vibrationsPressure changesOther conditions encountered during transportWeatherTemperatureRough handlingJust In Time TrainingDiagnostic Sampling: Overview
10. Packaging BasicsPrevent breakagePad and protect from rough handlingPrevent spills/leaksDouble waterproof barriersAbsorbent materialTemperatureLabelingPotentially hazardous materialsJust In Time TrainingDiagnostic Sampling: Overview
11. Preventing Breakage and SpillsPlastic versus glass containersPad primary sample container(s)Primary watertight barrierAbsorbent materialSecondary watertight materialRigid outer material (box)Do not pack formalin in same box with non-formalin samplesJust In Time TrainingDiagnostic Sampling: Overview
12. PackagingJust In Time TrainingDiagnostic Sampling: Overview
13. Temperature RequirementsWhole blood: on iceSerum: ice or dry iceSwabsCulture: icePCR: ambient Tissue samplesFresh: ice or dry iceFormalin fixed: ambientMaintain the cold chainJust In Time TrainingDiagnostic Sampling: Overview
14. Ice, Dry Ice, and FormalinIceArrival within 24 hoursIce packs onlyProtect samples fromdirect contactDry ice “Dry ice” label on boxCategory A shipmentsSubmission form in separate plastic bagJust In Time TrainingDiagnostic Sampling: Overview
15. Sample Shipping
16. Diagnostic Sample ShippingDOT hazardous material definition:Any material that is capable of causing risk to health, safety, and property during transportationIncludes infectious substancesRegulated by DOT Hazardous Materials Regulations (HMR)Section 49 Code of Federal RegulationsJust In Time TrainingDiagnostic Sampling: Overview
17. Sample ShippingShipping categoryCategory A: Infectious substances, Affecting animals only: UN 2900African swine fever virus, Foot and mouth disease virusAffecting humans: UN 2814Bacillus anthracis, Brucella melitensisDeclaration of dangerous goodsCategory B: Biological MaterialUN 3373Just In Time TrainingDiagnostic Sampling: Overview
18. UN Package Certification Mark Just In Time TrainingDiagnostic Sampling: Overview
19. Packaging: Category B Infectious SubstancesJust In Time TrainingDiagnostic Sampling: Overview
20. Packaging: Category A Infectious SubstancesJust In Time TrainingDiagnostic Sampling: Overview
21. Quantity LimitationsPassenger aircraft/railCargo aircraftCategory AInfectious substances, affecting humans (UN 2814) or animals (UN 2900)50 ml or50 g4 L or4 kgCategory B4 L or4 kg4 L or4 kgJust In Time TrainingDiagnostic Sampling: Overview
22. USDA-APHIS NVSLNVSL-AmesUSDA-APHIS-NVSL1920 Dayton Avenue Ames, IA 50010515-337-7266NVSL-FADDL-NYPlum IslandUSDA-APHIS-FADDL 40550 Route 25Orient, NY 11957631-323-3256Just In Time TrainingDiagnostic Sampling: OverviewNational Animal Health Laboratory Network (NAHLN) and USDA-APHIS approved laboratories http://www.aphis.usda.gov/animal_health/nahln/labs.shtmlwww.aphis.usda.gov/animal_health_lab_info_services/
23. USDA-APHIS NVSLDiagnostic Sample PrioritizationPriority 1: High SuspicionHighly likely a FAD or EDICall before submittingPriority 2: Intermediate SuspicionPossiblePriority 3: Low SuspicionUnlikelyPriority A:Animals in commerce being held pending resultsCall before submittingJust In Time TrainingDiagnostic Sampling: Overview
24. CarriersNot all carriers will ship biological materialsCarriers must have security plan when shipping certain agentsSubpart I of Part 172 of the HMRChose carrier that allows trackingEnsure proper equipment/vehiclesPrompt and reliable delivery is keySecuritySample viabilityJust In Time TrainingDiagnostic Sampling: Overview
25. ResourcesUSDA-APHISUSDA-APHIS National Veterinary Services Laboratorieshttp://www.aphis.usda.gov/animal_health/lab_info_services/USDA-APHIS VS Memorandum No. 580.4: Procedures for the Investigation of Potential Foreign Animal Disease/Emerging Disease Incidents(FAD/EDI)http://www.aphis.usda.gov/animal_health/lab_info_services/downloads/VS_Memo580_4.pdfDepartment of Transportation49 CFR 172: Pipeline and Hazardous Materials Safety Administration, Department of Transportation – Hazardous Materials Regulations. http://ecfr.gpoaccess.gov/cgi/t/text/text-idx?c=ecfr&tpl=/ecfrbrowse/Title49/49cfr172_main_02.tplTransporting Infectious Substances Safely http://www.phmsa.dot.gov/staticfiles/PHMSA/DownloadableFiles/Files/Transporting_Infectious_Substances_brochure.pdfJust In Time TrainingDiagnostic Sampling: Overview
26. AcknowledgmentsDevelopment of this presentation was by the Center for Food Security and Public Health at Iowa State University through funding from the Multi-State Partnership for Security in AgricultureAuthors: Amber Stumbaugh, MS; Dan Taylor, DVM, MPH Reviewers: Glenda Dvorak, DVM, MPH, DACVPM