Marwah M. N. AL-Kanan and Iman A. J. - PowerPoint Presentation

1. Marwah M. N. AL-Kanan and Iman A. J. Al-TimimiDepartment of Chemistry, College of Science, University of Basrah, Basrah, IraqM. G. González-Pedroza et al., “Biogeneration of silver nanoparticles from Cuphea procumbens for biomedical and environmental applications,” Sci Rep, vol. 13, no. 1, p. 790, Jan. 2023, doi: 10.1038/s41598-022-26818-3. J. I. Orege, S. B. Adeyemi, B. B. Tiamiyu, T. O. Akinyemi, Y. A. Ibrahim, and O. B. Orege, “Artemisia and Artemisia-based products for COVID-19 management: current state and future perspective,” Advances in Traditional Medicine. Springer, 2021. doi: 10.1007/s13596-021-00576-5. A. Adhikari et al., “Green Synthesis of Silver Nanoparticles Using Artemisia vulgaris Extract and Its Application toward Catalytic and Metal-Sensing Activity,” Inorganics (Basel), vol. 10, no. 8, Aug. 2022, doi: 10.3390/inorganics10080113 ReferencesInorganic metal Ag nanoparticles (NPs) have recently received great attention owing to their biological and biomedical applications. Spherical Ag NPs were green synthesized using brown fresh Artemisia annua flowers extract in water as a reducing and a capping agent, and as a medicinal plant. The surface morphology of Ag NPs was characterized by TEM, EDX, FTIR, UV-Vis, DLS and XRD. The average size of Ag NPs is ~12 nm. The maximum activity result of DPPH scavenging was determined as 77% at a concentration of 97.02 ug/mL. The biocompatibility of the Ag NPs was greatly improved with no cytotoxic effect observed. The Ag NPs/ Artemisia annua flowers extract exhibited 72% neuraminidase inhibitory activity against influenza virus which improved with increased concentration (Ag NPs IC50 is 15.97 ±0.91). As a result, Ag NPs could be used as nanomedicine with enhanced antioxidant, antihaemolytic and antiviral properties. AbstractIntroductionBrown fresh Artemisia annua flowers (A. annua) were purchased from the market (Basrah, Iraq). Human blood was obtained from University of Basrah. The reagents used included: Silver nitrate (AgNO3, Alfa Aesar, 99.9%),and 2,2-diphenyl-1-picrylhydrazyl (DPPH procedure, Sigma– Aldrich, USA).Discussion Silver NPs exhibit unusual properties, leading to their application in biotechnology as electronics, sensors and optics. These properties and applications of Ag NPs depend on their size, particle morphology, surface chemistry and shape. The emergence of the medicinal plant Artemisia annua (A. annua) as an effective antiviral agent is based on its ability with interesting structures and morphologies due to different natural-selection functions. Many researchers reported that A. annua leaf extract show antiviral activity against COVID-19.This paper aims to study the use A. annua flowers extract as a valuable resource in producing biocompatible Ag NPs for various medical applications. Some of these applications in question are its antiviral and antioxidant use, which will be investigated to confirm the efficacy of the produced Ag NPs.Bio-generation of Ag nanoparticles using green chemistry for enhanced antioxidant and Antiviral ActivitiesMaterial & Methods Figure 1: TEM micrograph of: a) whole Ag NPs inside of A.ext xylem, b,d) Ag NPs with NP size average (20-40 nm) and C) Ag NPs/A.ext at RT and Histogram of AgNPs showing the particle size distribution (PSD). Figure 2: Antiviral activity of Ag NPs/A.ext at various concentrations (A=6.06 ug/mL, B= 8.08 ug/mL, C=12.13 ug/mL and D=24.25 ug/mL). Figure 3: Antioxidant activity of Ag NPs/A.ext against the DPPH: The concentrations of Ag NPs/A./extr. (A=6.06, B= 8.08, C=12.13 and D=24.25) ug/mL and ascorbic acid as standard (20mg/mL). Figure 4: UV-Vis spectra for: A.ext at (272 and 225nm), and SPR of Ag NPs at 410 nm