Dear ColleaguesThe Xema Corporate Group is one of the world146s leadin - PDF document

1 1 Dear Colleagues,The Xema Corporate Gro
1 Dear Colleagues,The Xema Corporate Group is one of the world’s leading biological companies providing human tumor biomarker native proteins and corresponding monoclonal antibodies with  2014 Product Catalogue 2 Product ListNatural antigens and their derivativesHuman Thyrogloblin (TG) puriedHuman Prolactin (PRL) standard gradeHuman Trypsin (IRT) standard gradeNEW PRODUCTHuman Uromodulin (Tamm-Horsfall protein) puriedNEW PRODUCT ........HE4 standard grade (WFDC2, WAP5)NEW PRODUCTHuman Prostate Specic Antigen (PSA) puriedHuman CA-125 (MUC16) Antigen puriedHuman CA242 Antigen puriedNEW PRODUCTHuman CA-199 (sialyl-Lewis a) Antigen puriedHuman Carcinoembryonic Antigen (CEA) puriedHuman Alpha-Fetoprotein (AFP) highly puried .............................................................................Human CA15-3 Antigen, standard gradeHuman NSE, standard grade

2
NEW PRODUCTHuman CYFRA 21-1 (Cytokeratin 19) antigen, standard gradeHuman CA72-4 Antigen standard gradeHuman Placental Lactogen (hPL), standard gradeNEW PRODUCTHuman C-reactive protein (CRP) puriedHuman Serum amyloid A (SAA), puriedNEW PRODUCTHuman Myeloperoxidase (MPO) puriedRecombinant Yeast HBsAg, ayw1 subtype puriedMonoclonal antibodiesMurine Monoclonal Antibodies to human IgG gamma-chainNEW PRODUCTMurine Monoclonal Antibodies to human IgM mu-chainNEW PRODUCTMurine Monoclonal Antibodies to Serum AlbuminsNEW PRODUCTMurine Monoclonal Antibodies to Human Haemoglobin (Hb)NEW PRODUCT18Murine Monoclonal Antibodies to Human Uromodulin (Tamm-Horsfall protein, THP)NEW PRODUCT .....18Murine Monoclonal Antibodies to Hepatitis B Surface Antigen (HBs)18Murine Monoclonal Antibodies to human GH18Murine Monoclonal Antibodies to human ProlactinMurine Monoclonal Antibodies to human FSH/LH/HCG alpha chain ...................................

3 ...Murine Monoclonal Antibody to human L
...Murine Monoclonal Antibody to human Luteinizing Hormone, LH beta chain specicMurine Monoclonal Antibody to human LH/ HCG, beta chain (LH/HCG) ...............................Murine Monoclonal Antibody to human Follicle Stimulating Hormone(FSH)................................................................................................................................Murine Monoclonal Antibodies to Human Chorionic Gonadotropin, HCG beta chain specic........................................................................................................................... 3 Murine Monoclonal Antibodies to Human Chorionic Gonadotropin, FREE beta chain (free beta HCG)......................................................................................................................Murine Monoclonal Antibody to human Thyroid Stimulating Hormone beta-subunit, TSH beta chain specic

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NEW PRODUCTMurine Monoclonal Antibodies to human Alpha-Fetoprotein (AFP) .......................................22Murine Monoclonal Antibodies to human IgE epsilon-chain22Murine Monoclonal Antibody to ProgesteroneMurine Monoclonal Antibody to TestosteroneMurine Monoclonal Antibody to CortisolMurine Monoclonal Antibody to thyroxine (T4)Murine Monoclonal Antibody to 17-OH ProgesteroneNEW PRODUCTMurine Monoclonal Antibody to human PSA freeMurine Monoclonal Antibodies to human PSA totalMurine Monoclonal Antibodies to antigen CA125 (ISOBM workshop TD1) NEW PRODUCTMurine Monoclonal Antibodies to carcinoembryonic antigen (CEA, ISOBM TD8 worshop)..........................................................................................................

5 .................................Murine
.................................Murine Monoclonal Antibodies to Polymorphic Epithelial Mucin (PEM, MUC1)Murine Monoclonal Antibodies to CA19.9Murine Monoclonal Antibodies to human thyroglobulin (TG)Murine Monoclonal Antibodies to CYFRA21-1NEW PRODUCTMurine Monoclonal Antibodies to HE4NEW PRODUCTPolyclonal antibodies and antisera ...............................................................................................................Antibodes to rabbit Ig (whole molecule)Antibodies to mouse Ig (whole molecule)Rabbit anti-human HaemoglobinRabbit anti-human Uromodulin (Tamm-Horsfall Protein, THP)Rabbit anti-human IgE epsilon-chain, human IgG adsorbedRabbit Antiserum to Dihydroepiandrosterone Sulphate (DHEA-S)Goat antibody to carcinoembryonic antigen (CEA)HRPO conjugated LMW hormonesProgesterone-11-hemisuccinyl-HRPOEstradiol-6-carboxymethyloxime-HRPO .............................................................................................Testosterone-3-carboxymethyloxime-HRPOCortisol-3-carboxymethyl

6 oxime-HRPOT3-CONH-HRPO .................
oxime-HRPOT3-CONH-HRPO ............................................................................................................................................T4-CONH-HRPO..........................................................................................................DHEA-3-hemisuccinyl-HRPO17-OH-Progesterone-3-CMO-BSA 4 Streptavidin - HRPO conjugateReagent sets ...........................................................................................................................................................Other reagents and auxiliary components for EIA:- Protein A.....................................................................................................................41- Protein G.....................................................................................................................41- mAb to human IgG141- mAb to human IgG241- mAb to human IgG341- mAb to human IgG441- mAb to human alfa-chain IgA41- mAb to free kappa-chain human IgG41- mAb to free lambda-chain h

7 uman IgG41- EIA stabilizer for tracer41-
uman IgG41- EIA stabilizer for tracer41- EIA sample dilution buer ......................................................................................................................41- EIA coating buers (pH 4.1; pH 7.0; pH 9.0)41- EIA stabilizer for microplates .................................................................................................................41- Additive to decrease false positives in ELISA (MOPC IgG)41 5 Natural antigens and their derivatives Human Thyroglobulin (TG) puriedCatalogue No.Source and infection testingThyroid tissue, surgery, tissue extracts tested negative for HBs antigen, HCV Ab, HIV I/II Ab in tissue extract by FDA approved EIA methods. Remark on purication methodGel ltration and ion-exchange chromatographyMeasurement of concentration and specic activityOD280 and EIA (XEMA)FormatSolution frozen / freeze driedPurity data% by SDS-PAGE; essentially free of TPO BuerDried from PBS, pH 7.4–7.6, 0.05StorageRefrigerate at +2&

8 #133;+8 °C for 12 monthsor store at -20
#133;+8 °C for 12 monthsor store at -20 °C for at least 3 yearsApplicationsSolid phase antigen in immunoassays (coating Ag in EIA RK132 XEMA), iodination Human Prolactin (PRL) standard gradeCatalogue No. Source and infection testingPituitary glands, tissue extracts tested negative for HBs antigen, HCV Ab, HIV I/II Ab in tissue extract by FDA approved EIA methods. Remark on purication methodPuried without immunoanity stepMeasurement of concentration and specic activityEIA (Xema)FormatSolution frozen / freeze driedPurity data�60 % by SDS-PAGEBuerDried from PBS, pH 7.4-7.6, 0.05% sodium azide.StorageFrozen at -20 °C or below for at least 3 yearsApplicationsCalibrator in PRL assays (Xema RK206) 6 Human Trypsin (IRT) standard gradeNEW PRODUCTCatalogue No. Source and infection testingPancreatic tissue, surgery, tissue extracts tested negative for HBs antigen, HCV Ab, HIV I/II Ab in tissue extract by FDA approved EIA methods. Remark on purication methodGel ltration and

9 ion-exchange chromatographyMeasurement
ion-exchange chromatographyMeasurement of specic activityEIA (Xema)FormatSolution frozen / freeze driedPurity data�25 % by SDS-PAGEBuerStorageStore at -20 °C for at least 3 yearsApplicationsCalibrator in IRT assays (Xema RK242) Human Uromodulin (Tamm-Horsfall protein) puriedNEW PRODUCTCatalogue No. Source and infection testingUrine Remark on purication methodGel ltration and ion-exchange chromatographyMeasurement of concentration and specic activityFormatSolution liquidPurity data�90 % by SDS-PAGE BuerPBS, pH 7.4–7.6, 0.05 % sodium azide. StorageRefrigerate at +2-8 °C for 12 months or store at -20 °C for at least 3 yearsApplicationsImmunogen, calibrator for immunoassays, in vitro studies 7 HE4 standard grade (WFDC2, WAP5)NEW PRODUCTCatalogue No. Source and infection testingAscitic uid, ovarian carcinoma tested negative for HBs antigen, HCV Ab, HIV I/II Ab in tissue extract by FDA approved EIA methods. Remark on purication methodGel l

10 trationMeasurement of concentration and
trationMeasurement of concentration and specic activityOD280 and EIA (CanAg)FormatSolution frozenPurity data�25 % by SDS-PAGEBuerStorageFrozen at -20 °C or below for at least 3 yearsApplicationsCalibrator in inmmunoassays Human Prostate Specic Antigen (PSA) puriedCatalogue Source and infection testingDonor seminal uid tested negative for HBs antigen, HCV Ab, HIV I/II Ab in tissue extract by FDA approved EIA methods. Remarks on purication methodSalt precipitationMeasurement of concentration and specic activityOD280, EIA (XEMA)FormatSolution frozenPurity data% by SDS-PAGEBuerStorageFrozen at -20 °C or below; do not keep in liquid form for longer than 48 hoursApplicationsImmunogen, calibrator for immunoassays (EIA RK221 XEMA), further purication, in vitro studiesSupplementary informationThis product is available as sterile puried form and as standard grade form (recovery ca. 20-40by total protein) 8 Human CA-125 (MUC16) Antigen puriedCatalogue So

11 urce and infection testingAscitic u
urce and infection testingAscitic uid, adenocarcinoma, single patient tested negative for HBs antigen, HCV Ab, HIV I/II Ab in tissue extract by FDA approved EIA methods. Remarks on purication methodPuried by salt extractions and subsequent chromatography without perchloric acid treatmentMeasurement of concentration and specic activityOD280, immunoassays (Roche Elecsys, XEMA)FormatSolution frozenPurity dataSpecic activit�y per mg 100 kU/mg total proteinPurity was detected by SDS-PAGE: the absence of the bands in SDS-PAGE (Coomassie R250 as dye) means the purity of the antigen CA125 BuerStorageFrozen at -20 °C or belowApplicationsImmunogen, calibrator for immunoassays (EIA RK222 XEMA), in vitro studiesSupplementary informationThis product is available as standard grade form (recovery ca. 20-40% by total protein)Concentration range Human CA242 Antigen puriedNEW PRODUCTCatalogue Source and infection testingMetastatic liver, carcinoma, single patient tested negative f

12 or HBs antigen , HCV Ab, HIV I/II Ab in
or HBs antigen , HCV Ab, HIV I/II Ab in tissue extract by FDA approved EIA methods. Remarks on purication methodGel ltration, ion-exchange chromatography. Puried without perchloric acid treatmentMeasurement of concentration and specic activityOD280, immunoassay (CanAg)FormatSolution frozenPurity dataPurity was detected by SDS-PAGE : the absence of the bands in SDS-PAGE (Comassie R250 as dye) means, the purity of the antigen CA242BuerPBS, pH 7.2–7.4, 3 % sucrose, 0.05 % sodium azideStorageFrozen at -20 °C or belowApplicationsImmunogen, calibrator in immunoassays, in vitro studiesSupplementary informationThis product is available as standard grade form (recovery ca. 20–40 % by total protein)Concentration range 9 Human CA-199 (sialyl-Lewis a) Antigen puriedCatalogue Source and infection testingMetastatic liver, carcinoma, single patient tested negative for HBs antigen, HCV Ab, HIV I/II Ab in tissue extract by FDA approved EIA methods. Remarks on purication methodG

13 el ltration, ion-exchange chromatog
el ltration, ion-exchange chromatography. Puried without perchloric acid treatmentMeasurement of concentration and specic activityOD280, immunoassays (Roche Elecsys, XEMA)FormatSolution frozenPurity dataPurity was detected by SDS-PAGE : the absence of the bands in SDS-PAGE (Comassie R250 as dye) means, the purity of the antigen CA199 Buer% sucrose, 0.05StorageFrozen at -20 °C or belowApplicationsImmunogen, calibrator in immunoassays (EIA RK223 XEMA), in vitro studies Supplementary informationThis product is available as standard grade form (recovery ca. 20–40% by total protein)Concentration range Human Carcinoembryonic Antigen (CEA) puriedCatalogue Source and infection testingMetastatic liver, carcinoma, single patient tested negative for HBs antigen, HCV Ab, HIV I/II Ab in tissue extract by FDA approved EIA methods. Remarks on purication methodPuried using immunoanity column Measurement of concentration and specic activityOD280, immunoassays (Roche Elecsy

14 s, XEMA)FormatSolution frozenPurity data
s, XEMA)FormatSolution frozenPurity data% by SDS-PAGE, diuse band in the range ; poorly stained by Coomassie R250Buer20 mM Tris, 0.1M NaCl, 0,05StorageFrozen at -20 °C or belowApplicationsImmunogen, calibrator in immunoassays (EIA RK224 XEMA), in vitro studies Supplementary informationThis product is available as standard grade form % by SDS-PAGE, diuse band in the range 180–200 kD; main impurities: albumin, haptoglobin) and sterile for in vitro studies (e.g. lymphocyte stimulation) 10 Human Alpha-Fetoprotein (AFP) highly puried Catalogue Source and infection testingCord serum tested negative for HBs antigen, HCV Ab, HIV I/II Ab in tissue extract by FDA approved EIA methods. Remarks on purication methodPuried by special method to ensure maximal recovery of immunoreactive proteinMeasurement of concentration and specic activityOD280, immunoassays (Roche Elecsys, XEMA)FormatSolution frozen / freeze driedPurity data% of AFP isoforms and fragments, % of single 70 kDa AFP

15 band by SDS-PAGEBuerStorageFrozen
band by SDS-PAGEBuerStorageFrozen at -20 °C or belowApplicationsImmunogen, calibrator in immunoassays (EIA RK225 XEMA), in vitro studies Supplementary informationThis product is also available as standard grade form % content of AFP band in SDS-PAGE. Albumin and prealbumin are the main impurities) for further purication Human CA15-3 Antigen, standard gradeCatalogue Source and infection testingHuman uids tested negative for HBs antigen, HCV Ab, HIV I/II Ab in tissue extract by FDA approved EIA methods.Remarks on purication methodHigh speed centrifugation, salt precipitation, delipidizationMeasurement of concentration and specic activityEIA (CanAg)FormatSolution liquidPurity dataBuer1M NaCl, 1% Triton X100, 0.1StorageRefrigerate at +2…+8 °C for 12 monthsApplicationsImmunogen, further purication, calibrator in immunoassays Supplementary informationThis product is unavailable as puried formConcentration range 11 Human NSE, standard gradeNEW PRODUCTCatalogue Sourc

16 e and infection testingHuman tissue test
e and infection testingHuman tissue tested negative for HBs antigen, HCV Ab, HIV I/II Ab in tissue extract by FDA approved EIA methods. Remarks on purication methodIon exchangeMeasurement of concentration and specic activityFormatSolution liquidPurity dataBuer0.2M KCl, 10 mM MgCl2, 15 mM Tris, pH 6, StorageRefrigerate at +2…+8 °C for 12 monthsApplicationsImmunogen, further purication, calibrator in immunoassays Supplementary informationThis product is unavailable as puried form Human CYFRA 21-1 (Cytokeratin 19) antigen, standard gradeCatalogue Source and infection testingHuman mammary carcinoma cell line MCF7Remarks on purication methodNon processed induced cell culture mediumMeasurement of concentration and specic activityImmunoassay (Roche Elecsys)FormatSolution frozenPurity dataBuerCell culture medium as is StorageFrozen at -20 °C or belowApplicationsFurther purication, immunoassay calibrationSupplementary informationThis product is unavailable as puri

17 ed formConcentration range 12 Human
ed formConcentration range 12 Human CA72-4 Antigen standard gradeCatalogue Source and infection testingMetastatic liver, carcinoma, single patient tested negative for HBs antigen, HCV Ab, HIV I/II Ab in tissue extract by FDA approved EIA methods. Remarks on purication methodPuried without perchloric acid treatmentMeasurement of concentration and specic activityImmunoassay (Roche Elecsys)FormatSolution frozenPurity data% by SDS-PAGE, diuse band in the range 40 kD; main impurity: albuminBuerStorageFrozen at -20 °C or belowApplicationsImmunogen, calibrator in immunoassays, in vitro studies Supplementary informationThis product is unavailable as puried formConcentration range Human Placental Lactogen (hPL), standard gradeNEW PRODUCTCatalogue Source and infection testingHuman placentaRemarks on purication methodPuried using immunoanity columnMeasurement of concentration and specic activityImmunoassay (DRG)FormatSolution frozenPurity dataBuer100 mM Tri

18 s, acetate Na, 0.5 M NaCl, 1 % azide NaS
s, acetate Na, 0.5 M NaCl, 1 % azide NaStorageFrozen at -20 °C or belowApplicationsFurther purication, immunoassay calibrationSupplementary informationThis product is unavailable as puried formConcentration range 13 Human C-reactive protein (CRP) puriedCatalogue Source and infection testingSeptic plasma tested negative for HBs antigen, HCV Ab, HIV I/II Ab in tissue extract by FDA approved EIA methods. Remarks on purication methodPuried by specic ligand anityMeasurement of concentration and specic activityTotal protein by OD280 nm, immunoassay (EIA XEMA)FormatSolution liquidPurity data% by SDS-PAGE; specically depleted from Serum amyloid ABuer0.3 M NaCl, 20 mM Tris, pH 8.0, 0.1StorageRefrigerated at +2…+8 °C for at least 3 yearsMay form a reversible precipitate.Do nor freeze!ApplicationsCalibrator in immunoassays, immunization, in vitro studiesSupplementary informationThis product is unavailable as standard grade form Human Serum amyloid A (SAA), puri

19 edNEW PRODUCTCatalogue Source and i
edNEW PRODUCTCatalogue Source and infection testingSeptic plasma tested negative for HBs antigen , HCV Ab, HIV I/II Ab in tissue extract by FDA approved EIA methods. Remarks on purication methodPuried by specic ligand anityMeasurement of concentration and specic activityTotal protein by OD280 nmFormatSolution liquidPurity data�95 % by SDS-PAGE; specically depleted from C-reactive proteinBuer0.3M NaCl, 20 mM Tris, pH 8.0, 0.1 % azide NaStorageRefrigerated at +2…+8 °C for at least 3 yearsMay form a reversible precipitate.Do nor freeze!ApplicationsCalibrator in immunoassays, immunization, in vitro studiesSupplementary informationThis product is unavailable as standard grade form 14 Human Myeloperoxidase (MPO) puriedCatalogue No. Source and infection testingDonor leukocyte mass, tissue extracts tested negative for HBs antigen , HCV Ab, HIV I/II Ab in tissue extract by FDA approved EIA methods. Remarks on purication methodAnity puriedMeasu

20 rement of concentration and specic
rement of concentration and specic activityFormatSolution frozenPurity data�95 % by SDS-PAGE; essentially free of lactoferrinBuer0.5 M NaCl, 20 mM Tris, pH 7.6StorageStore at -20 °C or -70 °C for at least 3 yearsApplicationsSolid phase antigen in immunoassays (coating Ag in EIA RK133 Xema), in vitro research Recombinant Yeast HBsAg, ayw1 subtype puriedCatalogue RY01SourcePichia pastoris yeast, recombinantRemarks on purication methodPuried without immunoanity step with maximal recovery of immunoreactivityMeasurement of concentration and specic activityLowry, immunoassays (Abbott Murex EIA)FormatSolution frozen Purity dataTwo bands of monomeric plus dimeric forms give c�ombined 95% by SDS-PAGEBuer0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05StorageRefrigerate up to 1 month, freeze for longer periodStrictly avoid freeze thaw cycles.ApplicationsSolid phase antigen in immunoassay, calibrator in immunoassay, in vitro studies, HRPO conjugationSupplementary informati

21 onThis product is available as HRPO conj
onThis product is available as HRPO conjugate and sterile for in vitro studies 15 Monoclonal antibodies PresentationConcentration rangePurity data% by SDS-PAGEBuer0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05Measurement of concentration Remarks on purication methodIon exchangeStorageRefrigerate at +2...+8 °C for not longer than 12 months. FormatSolution liquid Murine Monoclonal Antibodies to human IgG gamma-chainNEW PRODUCTCatalogue No/ClonesSee table belowIsotypeImmunogenPuried natural IgGSpecicity and anity dataAll antibodies selected for high anity for human IgG whole molecule, negative for IgM.None of the clones shows considerable selectivity for IgG subclass.ApplicationsImmunoassays; WB.Supplementary informationProducts are available as HRPO conjugates (e.g. EIA RK101-RK106, RK131, RK132 Xema) Recognition of animal IgGClone #Fragment specicity*caninefelinebovineporcineXA6XG1XG3XG4XG6XG7XG8XG9XG14XG23XG33XG34XG36 16 Clone #Fragment specicity*caninefelinebovineporcineX

22 G47XG55XG56XG69XG74XG76XG78XG99XG100XG11
G47XG55XG56XG69XG74XG76XG78XG99XG100XG114* preferred binding Murine Monoclonal Antibodies to human IgM mu-chainNEW PRODUCTCatalogue No/ClonesSee table belowIsotypeImmunogenPuried natural IgMSpecicity and anity dataAll antibodies selected for high anity for human IgM whole molecule, negative for IgG and IgAApplicationsImmunoassays; WB. canine canine feline porserum 17 Murine Monoclonal Antibodies to Serum AlbuminsNEW PRODUCTCatalogue No/ClonesSee table belowIsotypeImmunogenPuried natural IgMSpecicity and anity dataAll antibodies selected for high anity for human or bovine ApplicationsImmunoassays; WB. mAb(HSA)cow (BSA)goatdogcatrabbitrat 18 Murine Monoclonal Antibodies to Human Haemoglobin (Hb)NEW PRODUCTCatalogue No/ClonesSee table belowImmunogenPuried human haemoglobin Clone IdIsotypespecies specicity*remarkmatched with for FOB testmatched with for FOB testmatched with for FOB test*non-reactive to bovine, porcine, ovine, equine and avian Hb Murine Monoclon

23 al Antibodies to Human Uromodulin (Tamm-
al Antibodies to Human Uromodulin (Tamm-Horsfall protein, THP)NEW PRODUCTCatalogue No/ClonesXT11, XT12, XT16IsotypeImmunogenPuried human uromodulinSpecicity and anity dataAll antibodies selected for species specicity (non-reactive to canine, feline, bovine and equine THP)ApplicationsImmunoassays; WB. Murine Monoclonal Antibodies to Hepatitis B Surface Antigen (HBs)Catalogue /CloneIsotypeImmunogenPuried natural HBs antigenSpecicity and anity dataHigh anityApplicationsCapture in immunoassays; WB, HRPO conjugation Murine Monoclonal Antibodies to human GHCatalogue /CloneXGH6XGH7IsotypeImmunogenGrowth hormone, puried without immunoanity stepSpecicity and anity dataHigh anityApplicationsXGH7 antibodies of solid phase matches with XGH6 (EIA RK204 XEMA) 19 Murine Monoclonal Antibodies to human ProlactinCatalogue /ClonesIsotypeImmunogenProlactin, puried without immunoanity stepSpecicity and anity dataHigh anityApplicationsImmu

24 noassays, anity purication of
noassays, anity purication of Prolactin Murine Monoclonal Antibodies to human FSH/LH/HCG alpha chainCatalogue /ClonesIsotypeImmunogenPuried natural FSH alpha chainSpecicity and anity dataHigh anityApplicationsCapture matches XL1, XF2 (EIA RK202, RK203) Murine Monoclonal Antibody to human Luteinizing Hormone, LH beta chain specicCatalogue /ClonesIsotypeImmunogenPuried natural LHSpecicity and anity dataHigh anityApplicationsXL1 antibodies of solid phase matches with XF1(EIA RK2022, Xema) Murine Monoclonal Antibody to human LH/ HCG, beta chain (LH/HCG) Catalogue /ClonesIsotypeImmunogenPuried natural LHSpecicity and anity dataHigh anity; cross-reactive to HCG betaApplicationsXL1 antibodies of solid phase matches with XF1(EIA RK2022, Xema) Murine Monoclonal Antibody to human Follicle Stimulating Hormone (FSH)Catalogue /ClonesIsotypeImmunogenPuried natural FSHSpecicity and anity dataHigh anityApplicationsXF2 antibodies

25 of solid phase matches with XF1(EIA RK2
of solid phase matches with XF1(EIA RK2032, Xema) 20 Murine Monoclonal Antibodies to Human Chorionic Gonadotropin, HCG beta chain specicCatalogue /CloneIsotypeImmunogenPuried natural HCG Specicity and anity dataHigh anityNo cross reactivity with LH, TSH, FSHApplicationsImmunoassay XH51 antibodies of solid phase matches with XF1(EIA RK205, XEMA), WB Murine Monoclonal Antibodies to Human Chorionic Gonadotropin, FREE beta chain (free beta HCG)Catalogue /CloneIsotypeImmunogenPuried natural beta HCG Specicity and anity dataHigh anityNo cross reactivity with LH, TSH, FSHApplicationsImmunoassay XH95 antibodies of solid phase matches with XH220 (EIA RK235, XEMA) 21 Murine Monoclonal Antibody to human Thyroid Stimulating Hormone beta-subunit, TSH beta chain specicNEW PRODUCTCatalogue /ClonesXTB1 XTB63XTB2 XTB64XTB3 XTB73XTB9 XTB75XTB10 XTB80XTB11 XTB82XTB17 XTB94 XTB32IsotypeImmunogenPuried natural TSH betaSpecicity and anity dataHigh a

26 8;nityApplications: immunoassay pairs, s
8;nityApplications: immunoassay pairs, see table below coat\tracerXTB1*XTB2*XTB3*XTB9*XTB10*XTB11*XTB32*XTB1XTB2XTB3XTB9XTB10weakXTB11weakXTB17XTB32XTB33XTB63XTB64XTB73weakXTB75XTB77weakweakXTB80weakweakXTB82XTB94weakweak* XR HCGbeta in one step protocol 22 Murine Monoclonal Antibodies to human Alpha-Fetoprotein (AFP)Catalogue /CloneXA2IsotypeImmunogenPuried natural AFPSpecicity and anity dataHigh anityApplicationsXA2 antibodies of solid phase matches with XFP2 (EIA RK225 XEMA), WB, IHC Immunostaining with XFP2Immunostaining with XFP2of placentaof placenta Murine Monoclonal Antibodies to human IgE epsilon-chainCatalogue /CloneXTE4 (epsilon chain)IsotypeImmunogenPuried myeloma IgE (epsilon; alfa)Specicity and anity dataHigh anityApplicationsXTE4 antibodies of solid phase matches with AS200 (EIA RK200 XEMA), WBX5D4 antibodies of solid phase matches with X4F4 23 Murine Monoclonal Antibody to ProgesteroneCatalogue /CloneIsotypeImmunogenProgesterone-11(HS)-BSA Specic

27 ity and anity dataHigh anity,
ity and anity dataHigh anity, K = 3.0x10exp9 Mexp-1ApplicationsCapture antibodies in immunoassays matches T207X (EIA RK207 XEMA)Cross-reactivity data Cross-reactivity, Steroid 50% displacement of Progesterone-11-125-Iodine) Progesterone 10017-Hydroxyprogesterone 1.011-Hydroxyprogesterone 5-alpha-pregnane-3,20 dionene 10.5Corticosterone 0.01Pregnenolone 0.9Deoxycorticosterone 0.30Deoxycortisol 0.03Cortisol 0.002 Murine Monoclonal Antibody to TestosteroneCatalogue /CloneIsotypeImmunogenTestosterone-3-CMO-BSASpecicity and anity dataHigh anity, K = 2.5x10exp9 Mexp-1ApplicationsCapture antibodies in immunoassays matches T209X (EIA RK209 XEMA)Cross-reactivity data Cross-reactivity, Steroid 50% displacement of Testosterone-3-125-Iodine) Testosterone 1005-alpha-dehydrotestosterone16Androstendiol 1.0Androstendione 0.4Androsterone 0.1Dehydroepiandrosterone 0.1Progesterone 0.1Estradiol, Estriol 0.01Cortisol, Pregnenolone 0.01 24 Murine Mo

28 noclonal Antibody to CortisolCatalogue /
noclonal Antibody to CortisolCatalogue /CloneIsotypeImmunogenCortisol-3(CMO)-BSASpecicity and anity dataHigh anity, K = 1.7x10exp9 Mexp-1ApplicationsCapture antibodies in immunoassays matches T210X (EIA RK210 XEMA)Cross-reactivity data Cross-reactivity,Steroid 50% displacement of cortisol-3-125-Iodine) Cortisol 10011-Deoxycortisol 0.9Prednisolone 5.6 Corticosterone 0.611-Deoxycorticosterone 0.1Progesterone 0.117 –Hydroxyprogesterone .1Testosterone, Estradiol, Estriol .1Danazol 0.01 Murine Monoclonal Antibody to thyroxine (T4)Catalogue /CloneIsotypeImmunogenT4-3-CONH-BSASpecicity and anity dataHigh anity, K = 5.1x10exp9 Mexp-1ApplicationsCapture antibodies in immunoassays matches T212X (EIA RK212 (total T4), RK2142 (free T4) XEMA)NOT APPLICABLE FOR RIACross-reactivity data Cross-reactivity, Substance 50% displacement of T4-125-Iodine) L-Thyroxine 100D-Thyroxine 30T3 0.5 25 Murine Monoclonal Antibody to 17-OH ProgesteroneCatalogue /C

29 loneIsotypeIgG1ImmunogenProgesterone-11(
loneIsotypeIgG1ImmunogenProgesterone-11(HS)-BSA Specicity and anity dataHigh anityApplicationsCapture antibodies in immunoassays matches T217XCross-reactivity data Steroid Cross-reactivity, 17-Hydroxyprogesterone 100Progesterone 1.011-Deoxycorticosterone 2.3 Murine Monoclonal Antibody to human PSA freeCatalogue /CloneIsotypeImmunogenPuried natural PSASpecicity and anity dataHigh anityApplicationsImmunoassays (EIA RK231 XEMA) Murine Monoclonal Antibodies to human PSA totalCatalogue /CloneIsotypeImmunogenPuried natural PSASpecicity and anity dataHigh anityApplicationsPS2 antibodies of solid phase matches with PS6 (EIA RK221 (PSA total) XEMA); matches with PS1 (EIA RK231 (PSA free) XEMA), IHC Immunostaining with PS2:Immunostaining with PS2:benign prostatic adenoma prostatic adenocarcinoma 26 Murine Monoclonal Antibodies to antigen CA125 (ISOBM workshop TD1)NEW PRODUCTCatalogue /CloneEpitope specicity of CA125 monoclonal antibodies was

30 determined by competitive binding to en
determined by competitive binding to enzyme-labelled human single patient CA125 antigen. Antigen: natural human CA125 (lot CA23) isolated from single patient ovarian tumour ascitic uid; purication method did not include anity binding to monoclonal antibodies.Conjugate of human CA125 lot CA23 with HRPO enzyme (Faizyme, Lansdowne, RSA) was performed by perjodate method and was diluted 1:500 by EIA buer.The results show that mAb X306 clearly belongs to A1 (OC125 like) epitope group; mAb X75 and X325 show high homology to B1 (M11 like) group. Recommended pairs for immunoassays are:X306 solidX52-HRPO conjugate X325 solidX52-HRPO conjugateX306 solidX325-HRPO conjugate189 solid192-HRPO conjugateAnti-CA125 mAbs application: IHC Immunostaining with X325:Immunostaining with X325:ovarian cancer lang adenocarcinomaCross-inhibition of Xema CA125 mAbs and reference mAbs from TD1 workshop. Rows – liquid phase antibodies; columns – immobilized antibodies. Inhibition percentages (se

31 e table) were calculated by dividing the
e table) were calculated by dividing the average OD in actual wells by the average OD in control wells. Epitope group 27 Murine Monoclonal Antibodies to carcinoembryonic antigen (CEA, ISOBM TD8 workshop)Catalogue /CloneIsotypeImmunogenPuried natural CEASpecicity and anity dataHigh anity Applicationsantibodies of solid phase matches with 3C1 (EIA RK224 XEMA) AntibodyIsotypeSpeciesGold groupRemarkrecommended for IHC only4? 4’’recommended as tracerrecommended for IHCrecommended as captureSpecially recommended for lateral ow method* epitope group unknown, group 1 is the closest 28 anti-CEA mAbs application: IHC in metastasis of pancreatic carcinoma: CloneBrightnessCytoplasmic Membrane Background Staining PicturesSpecicity of anti-CEA mAbs according to NABM scheme (1C10?)2C5, 3C23C5, 3C6 3C13C43C9 Pic. 1 Pic. 2 Pic. 3 Pic. 4 Pic. 5 29 Murine Monoclonal Antibodies to Polymorphic Epithelial Mucin (PEM, MUC1)Catalogue /CloneIsotypeImmun

32 ogenPuried natural MUC1Specici
ogenPuried natural MUC1Specicity and anity dataHigh anity ApplicationsX108 antibodies of solid phase matches with X19 (EIA RK226, RK227 XEMA), IHC MUC1 (also known as PEM – polymorphic epithelial mucin) is an ubiquitous epithelial cell surface antigen expressed on wide variety of epitheliocytes. This antigen is also shed from the cells into body uids including saliva and normal serum.MUC1 molecule has a compex structure including extracellular 20 amino acid tandem repeat (VNTR region). This part of the molecule is most commonly used as the target for mAb binding. Within VNTR, the most popular locus for mAb recognition, is 7 aminoacid frame TRPAPGS. All well known reference mAbs (DF3, b12, B27.29) are directed to the epitope within this narrow frame.The natural mucins puried from tumors can contain extraordinarily high contents of MUC1 peptide but little to none CA15-3. Certainly, recombinant MUC1 peptides never contain “full” CA15-3 antigen.The ne dier

33 ences between anti-MUC1 mAbs have no ser
ences between anti-MUC1 mAbs have no serious inuence on their application in immunohistochemistry, immunoblotting, immuno-imaging or cytouorometry provided the MUC1 itself is the target molecule. No MUC1 mAb can replace the mammary specic mAbs eg 115D8 in cell sorting or stem cell enrichment. None of MUC1 mAbs can be considered as ‘mammary-specic’.To design the immunoassay for MUC1 in serum, there is three dierent strategies:To license the reference mAbs (present owner – Fujirebio Inc.) This was done by most ofthe major IVD makers.2.To construct a new pair from matched mAbs and prove its clinical relevance on serum panels. Certainly this assay cannot be called “CA15-3”. This was done eg by Roche with their MCA assay.To “copy” the specicity of the existing reference assays by combining two mAbs which “mimic” the specicity of the reference ones.This approach was used by CanAg. Their capture mAb is practically identical to 11

34 5D8; and tracer mAb strongly resembles D
5D8; and tracer mAb strongly resembles DF3. This assay can be considered as the good copy of the original CA15-3. 30 XEMA oers dierent mAbs to MUC1 which are able to “mimic” the specicity of the reference ones:MAb X19 recognizes preferably normally processed MUC1, similarly to mAb b12 (use inMCA Roche). In serum assays, this mAb can be used either in “self sandwich” or as thecapture or as the tracer.2.mAbs X23 and X77 prefer linear peptide epitope and is similar to reference mAb B27.29. Mab X108 is almost identical to mAb DF3.anti-MUC1 mAbs applications in IHC: Immunostaining Immunostaining Immunostainingwith X19 with X108 with X23of breast brocystic of prostate carcinoma of breast brocysticmastopathy (++) (++) mastopathy (++) 31 Murine Monoclonal Antibodies to CA19.9Catalogue /ClonesIsotypeImmunogenAnity pure CA19-9Specicity and anity dataHigh anity FormatSolution frozen StorageStore frozen at -20 °C ApplicationsImmu

35 noassay (EIA RK223 XEMA), IHCSupplementa
noassay (EIA RK223 XEMA), IHCSupplementary informationThis product is unavailable as HRPO conjugateanti-CA19.9 mAbs application: IHC Normal Pancreas Murine Monoclonal Antibodies to human thyroglobulin (TG)Catalogue /CloneXT21XT22XT23IsotypeImmunogenPuried natural thyroglobulinRemarks on purication methodGel ltrationSpecicity and anity dataHigh anity ApplicationsX5F9 vs X5E6 forms the sandwich with low interference to human auto-anti TG 32 Murine Monoclonal Antibodies to CYFRA21-1NEW PRODUCTCatalogue /CloneXC42, XC46, XC47,XC48, XC59, XC88, XC78, XC90, XC89 IsotypeXC46 - IgG2bImmunogenCYFRA21-1Specicity and anity dataHigh anity XC42, XC48, XC59, XC88, XC90 – KS19.1 homology (cross-inhibition) XC46 – high anity mAb binding soluble cytokeratins in broad variety of cell lines XC47,XC78, XC89 – KS19.2 homology (cross-inhibition) ApplicationsImmunoassay (EIA RK236 XEMA), WBXC42 matching with XC46 33 Murine Monoclonal Antibodies to HE4NEW PRODUCT

36 Catalogue /CloneIsotypeImmunogenHuman na
Catalogue /CloneIsotypeImmunogenHuman natural HE4Specicity and anity dataHigh anityApplicationsImmunoassay (EIA RK239 XEMA), WBEpitope maping Pairs recommended Group 34 Polyclonal antibodies and antisera PresentationConcentration rangeBuer0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05Measurement of concentration. Remarks on purication methodIon exchangeStorageRefrigerate at +2…+8 °C for not longer than 12 months. FormatSolution liquid Antibodes to rabbit Ig (whole molecule)Catalogue /CloneGoat or sheepImmunogenPuried rabbit gamma-globulinRemarks on purication methodanity purication on immobilized antigen Specicity and anity dataHigh anity, may cross-react to other mammalian IgApplicationsAnti-species capture in immunoassay (EIA RK208, RK211, RK213, RK215, RK217 Xema) Antibodies to mouse Ig (whole molecule)Catalogue /CloneGoat or sheepImmunogenPuried rabbit gamma-globulinRemarks on purication methodanity purication on immobilized

37 antigen Specicity and anity da
antigen Specicity and anity dataHigh anity, may cross-react to other mammalian IgApplicationsAnti-species capture in immunoassay (EIA RK208, RK211, RK213, RK215, RK217 Xema) Rabbit anti-human HaemoglobinCatalogue /CloneImmunogenPuried human haemoglobinRemarks on purication methodAnity purication on immobilized antigen Specicity and anity dataCross-reactive to other mammalian speciesApplicationsImmunoassay for FOB (vs Xema mAbs X305) http://www.xema-medica.come-mail: reagents@xema-medica@comPolyclonal antibodies and antisera 35 Rabbit anti-human Uromodulin (Tamm-Horsfall Protein, THP)Catalogue /CloneImmunogenPuried human THPRemarks on purication methodAnity purication on immobilized antigen Specicity and anity dataCross-reactive to other mammalian speciesApplicationsImmunoassay for THP (vs Xema mAbs) Rabbit anti-human IgE epsilon-chain, human IgG adsorbedCatalogue /CloneImmunogenPuried human myeloma IgE Remarks on puricatio

38 n methodAnity purication on im
n methodAnity purication on immobilized antigen Specicity and anity datanegative adsorptionApplicationsImmunoassay (EIA RK200 Xema), immunoblotting Rabbit Antiserum to Dihydroepiandrosterone Sulphate (DHEA-S)Catalogue /CloneImmunogenDHEA-3-hemisuccinyl-BSASpecicity and anity data% crossreactivity to DHEA ApplicationsImmunoassay (EIA RK215 XEMA) Goat antibody to carcinoembryonic antigen (CEA)Catalogue /CloneImmunogenPuried human carcinoembryonic antigen from tumor Remarks on purication methodanity purication on immobilized antigenApplicationsImmunoassay; immunochromatographic rapid test 36 HRPO conjugated LMW hormones Source materialHighly puried hormone derivative; HRPO�, RZ3.0Remarkson synthesisDepleted from unconjugated hormone by gel ltrationMeasurement of specic activityEIA titration. Titer not less than 1:5000 in direct binding FormatSolution liquid Buer% Tween-20, 0,5% solubilized casein, contains dyeStorageRefrigerate

39 at +2...+8 °C for not longer than 12 mo
at +2...+8 °C for not longer than 12 months. Presentation Progesterone-11-hemisuccinyl-HRPOCatalogue /CloneApplicationsTracer in immunoassay (EIA RK207 XEMA) Estradiol-6-carboxymethyloxime-HRPOCatalogue /CloneApplicationsTracer in immunoassay (EIA RK208 XEMA) Testosterone-3-carboxymethyloxime-HRPOCatalogue /CloneApplicationsTracer in immunoassay (EIA RK209 XEMA) Cortisol-3-carboxymethyloxime-HRPOCatalogue /CloneApplicationsTracer in immunoassay (EIA RK210 XEMA) T3-CONH-HRPOCatalogue /CloneApplicationsTracer in immunoassay (EIA RK211 (T3 total), RK213 (T3 free) XEMA) T4-CONH-HRPOCatalogue /CloneApplicationsTracer in immunoassay (EIA RK212 (T4 total), RK2142 (T4 free) XEMA) http://www.xema-medica.come-mail: reagents@xema-medica@comHRPO conjugated LMW hormones 37 DHEA-3-hemisuccinyl-HRPOCatalogue /CloneApplicationsTracer in immunoassay (EIA RK215 XEMA) 17-OH-Progesterone-3-CMO-BSACatalogue /CloneApplicationsTracer in immunoassay (EIA RK217 XEMA) Streptavidin - HRPO conjugateCatalogue /CloneSource materialFro

40 m Streptomyces avidinii. Anity puri
m Streptomyces avidinii. Anity puried.Remarks on synthesis methodConjugated by periodate method. Puried by gel ltration.Measurement of specic activityEIA titration. Titer not less than 1:10000 in direct FormatSolution liquid Buer% ProClin 300StorageRefrigerate at +2...+8 °C for not longer than 12 months. ApplicationsTracer in immunoassay; immunoblotting and immunohistochemistry 38 Reagent setsReagent sets are designed for construction of solid phase EIAs in OEM format. Xema supplies the listed immunoreagents and the manufacturing protocols. Optionally, XEMA supplies also the components or concentrated form of postcoating solutions, calibrator and conjugate diluents. Cat#Reagent setComponent #ExplanationSold separatelyas reagentRemarkanti-TPO EIACoating AgUnavailableas separate reagentCalibrator concentrate setUnavailableas separate reagentConjugate concentrateVariousanti-TG EIACoating AgCalibrator concentrate setUnavailableas separate reagentConjugate concentrateVariousanti-M

41 PO EIACoating AgCalibrator concentrate s
PO EIACoating AgCalibrator concentrate setUnavailableas separate reagentConjugate concentrateTotal IgE EIACoating mAbXTE4Calibrator concentrateUnavailableas separate reagentConjugate concentrateTSH EIACoating mAbVariousCalibrator concentrateConjugate concentrateVariousCoating mAbCalibrator concentrateUnavailableas separate reagentConjugate concentrateVariousCoating mAbCalibrator concentrateUnavailableas separate reagentConjugate concentrateCoating mAbXGH7Calibrator concentrateConjugate concentrateXGH6*HCG EIACoating mAbCalibrator concentrateUnavailableas separate reagentConjugate concentrateVariousUnavailableas separate reagent 39 Cat#Reagent setComponent #ExplanationSold separatelyas reagentRemarkPSA total EIACoating mAbCalibrator concentrateConjugate concentrateCA125 EIACoating mAbVariousCalibrator concentrateConjugate concentrateVariousCA199EIACoating mAbCalibrator concentrateConjugate concentrateUnavailableas separate reagentCoating mAbUnavailableas separate reagentCalibrator concentrateConjugate

42 concentrateCoating mAbXA2Calibrator conc
concentrateCoating mAbXA2Calibrator concentrateConjugate concentrateCA15-3 EIACoating mAbVariousCalibrator concentrateConjugate concentrateVariousPSA free EIACoating mAbCalibrator concentrateConjugate concentrateProgesterone First coating AbSecond coating AbCalibrator concentrateUnavailableas separate reagentConjugate concentrateConjugate buer additive concentrateUnavailableas separate reagentTestosterone First coating AbSecond coating AbCalibrator concentrateUnavailableas separate reagentConjugate concentrateConjugate buer additive concentrateUnavailableas separate reagentCortisol EIAFirst coating AbSecond coating AbCalibrator concentrateUnavailableas separate reagentConjugate concentrateConjugate buer additive concentrateUnavailableas separate reagentFirst coating AbSecond coating AbUnavailableas separate reagentCalibrator concentrateUnavailableas separate reagentConjugate concentrateConjugate buer additive concentrateUnavailableas separate reagent 40 Cat#Reagent setComponent #Explana

43 tionSold separatelyas reagentRemarkFirst
tionSold separatelyas reagentRemarkFirst coating AbSecond coating AbCalibrator concentrateUnavailableas separate reagentConjugate concentrateConjugate buer additive concentrateUnavailableas separate reagentT4 EIA freeFirst coating AbSecond coating AbCalibrator concentrateUnavailableas separate reagentConjugate concentrateUnavailableas separate reagentConjugate buer additive concentrateUnavailableas separate reagentDHEA-S EIAFirst coating AbSecond coating AbCalibrator concentrateUnavailableas separate reagentConjugate concentrateConjugate buer additive concentrateUnavailableas separate reagent17-OH-ProgesterFirst coating AbSecond coating AbCalibrator concentrateUnavailableas separate reagentConjugate concentrateConjugate buer additive concentrateUnavailableas separate reagentCRP ultrasensitive Coating mAbXA250Unavailable as separate reagentCalibrator concentrateConjugate concentrateXT250*Unavailable as separate reagentIRT EIACoating mAbVariousCalibrator concentrateConjugate concentrateVa

44 rious *Coating mAbUnavailable as separat
rious *Coating mAbUnavailable as separate reagentCalibrator concentrateConjugate concentrateUnavailable as separate reagentCoating mAbVariousCalibrator concentrateConjugate concentrateVarious 41 Other reagents and components for EIAOther reagents and components for EIA:Protein AProtein GmAb to human IgG1mAb to human IgG2mAb to human IgG3mAb to human IgG4mAb to human alfa-chain IgAmAb to free kappa-chain human IgGmAb to free lambda-chain human IgGEIA stabilizer for tracerEIA sample dilution buerEIA coating buers (pH 4.1; pH 7.0; pH 9.0)EIA stabilizer for microplatesAdditive to decrease false positives in ELISA (MOPC IgG)  2014 Product Catalogue 42 For note 43 For note 44 For note Natural antigens and their derivativeshttp://www.xema-medica.come-mail: reagents@xema-medica@com http://www.xema-medica.come-mail: reagents@xema-medica@comMonoclonal antibodies http://www.xema-medica.come-mail: reagents@xema-medica@comReagent sets http://www.xema-medica.come-mail: reagents@xema-medica@