Herbert H. BunzellTISSUESOXIDASE ACTIVITY IN PLANT WITH A NOTE ON THE - PDF document

Herbert H. BunzellTISSUESOXIDASE ACTIVIT
Herbert H. BunzellTISSUESOXIDASE ACTIVITY IN PLANT WITH A NOTE ON THE CAUSE OFHYDROGEN ION CONCENTRATIONS, OF PLANT JUICES AND THEIRBETWEEN THE OXIDASE ACTIVITY THE RELATIONSHIP EXISTING1916, 28:315-333.J. Biol. Chem.   http://www.jbc.org/content/28/1/315.citationAccess the most updated version of this article at  Alerts:   When a corre

ction for this article is posted• 
ction for this article is posted•  When this article is cited•  alerts to choose from all of JBC's e-mailClick here  ml#ref-list-1accessed free at by guest on November 19, 2020http://www.jbc.org/Downloaded from H. H. by guest on November 19, 2020http://www.jbc.org/Downloaded from 332 Oxidase Activity of Plant Juices many dif

ferent oxidase activities exhibited by p
ferent oxidase activities exhibited by plants. It seems probable that although the particular constituent is of general oc- currence, only an active modification of it is capable of giving oxidase reactions. This active modification would occur side by side with the inactive. When the oxidase activity of plant juices or tissues is being determine

d, a measure is obtained of the actual q
d, a measure is obtained of the actual quantity of the active material present; in live tissues this is replaced through the activity of protoplasm as fast as it is con- sumed. One is strongly tempted to think of proteins in this connection, since their properties seem to fulfill remarkably well the require- ments just laid down. They become dena

tured by acids, alcohol, and even in aqu
tured by acids, alcohol, and even in aqueous solutions after standing; they remain un- changed when dry, and occur in innumerable forms. The study of anaphylaxis has taught us that they may also exist in some extremely active modifications.14 Like all colloids, the proteins in the tissues may exist in a greater or lesser state of dispersion and h

a.ve accordingly a more or less active s
a.ve accordingly a more or less active surface. If we assume with Traube15 and others that it is this active surface which through surface tension action is capable of condensing oxygen as well as oxidizable materials at the surface, we can also under- stand how acids, poisons, alcohol, and heat, by diminishing the total surface of the disperse p

hase, markedly reduce the oxidase activi
hase, markedly reduce the oxidase activity. In widely different plants the colloids responsible for the reactions (possibly proteins) would assumedly be widely dif- ferent in their chemical and physical natures, as well as in their degree of dispersion, so that there would be great differences in the concentrations of active particles present amo

ng widely dif- ferent plants. Such a con
ng widely dif- ferent plants. Such a condensation of oxygen on the surface of the colloidal particles may explain the oxidase activity either by means of the greatly increased rate of reaction due to increased oxygen con- centration or by an increased oxidation potential of the oxygen when in such a condition. I4 An interesting discussion of the

biological individuality induced by prot
biological individuality induced by proteins is given by Robertson, T. B., liniv. California Publications, Physiology, 1911, iv, 25. 15 Traube, J., cber Katalyse, Arch. ges. Physiol., 1913, &ii, 309. by guest on November 19, 2020http://www.jbc.org/Downloaded from H. H. J. Biol. W. W. by guest on November 19, 2020http://www.jbc.org/Downloaded

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