Treatment Muhammad Bhatti Juan Lopez Megan Keniry University of Texas Rio Grande Valley Edinburg Texas USA Abstract The primary objective of this research ID: 779054
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Slide1
Radio
Frequency
Cancer
Treatment
Muhammad Bhatti,
Juan
Lopez,
Megan
Keniry
University
of
Texas
Rio Grande
Valley
Edinburg,
Texas
,
USA
Slide2Abstract
The
primary objective
of this research
endeavor is to study and
to understand the natural physics phenomenon of electromagnetic resonance
in
one
end
closed
cavity
for the
eventual
purpose of
cancer
treatment.
Radio
Frequency
waves
are discharged into
a
coaxial
cavity
filled with
a
small amount (1.6
mL)
of breast cancer
cells
(BT549) and
the reflection
as
well
as
the
power
input
is measured to
determine
the absorption
power
into
the
vitro
cancer
cell
experiment.
Slide3Conceptual
outlook
All matter contains natural
frequency of
oscillation
Such as a goblet resonating
at
natural
frequency
Once
goblet
reaches
natural
frequency,
goblet
self
destruct’s
Cell
can
be
oscillated
at
its
natural
frequency
Cell
can
be
destroy
by
reaching
resonance
frequency
Destruction
of cells
can
be
selective
Slide4Introduction
We
are interested in experimenting with
electromagnetic radiation in the
radio wavelength and
cancer cells. We are looking for
ways
to kill cancer
cells using
RF
while keeping
the
maximum
possible
number
of
healthy
cells
alive.
Slide5Method
ology
Coaxial cavity
with one end
closed
An PASCO Capstone Interface is then
used
with
software
A frequency
signal
Generator is
then operated
to
generate
833
MHz
Add 1.6
mL
sample
that
is being loaded into
the
waveguide
Input
wave
and
reflected
wave
powers
are recorded with
power
sensors
Slide6Equipment
Setup
Slide7Temperature
Gradient
Slide8T
heory
Free vibrations of an elastic body are called
natural
vibrations
occur at a frequency called the natural
frequency.
If
forced frequency
is
equal to the
natural
frequency,
the amplitude
of
vibration
increases many
times,
this is
known
as resonance
phenomenon.
Determine
the
reflected
wave
is
at
its
minimum
which
is
a
condition
for
the
cavity
resonance.
Slide9T
heory
Cell are
made
of moving atoms,
the vibrations inside cells travel as
waves,
at an
approximately
constant
velocity,
bouncing
back
and
forth
between
the sides
of
the
cell
membrane.If the length of the wave guide is L. To cause resonance, the phase ofa sinusoidal wave after a roundtrip must be equal to the initial phase, so the waves reinforce the oscillation or when absorption reaches maximum.
• 𝑓 = 𝑁𝑣
4 𝐿
𝑁𝜖
1
,
3
,
5
…
Slide10Experimental
Purpose
To determine
the effect of radio frequency
electromagnetic waves on human cell
cultures
in
vitro
cytotoxicity
testing
was
carried
out using human breast cancer cell
line
BT549 and human embryonic
healthy
kidney 293 cells
(HEK-293)
Slide11Cell
Preparation
The Trevigen
TACS MTT
Cell Proliferation Assay Kit
(Gaithersburg, MD) was utilized to assess
impacts on human cell proliferation and/or
viability
Cells were
grown under
standard tissue culture conditions
(5%
CO
2
,
37°C,
with 10%
FBS
and
pen/strep)
Cells were removed from plates with, centrifuged at 25°C (100 x g for 10 minutes) and re-suspended in fresh media at a density of 250,000 cells per mL prior to treatment
Slide12Cell
Treatment
Cells were treated at
833 MHz frequency
Cells
were in a control
environment
Total
time of exposure
was
20
minutes
Voltage
was
at
11
volts
for the
amplifier
input
Amperage of 0.62 amps being used by the amplifierControl mass of about 1.5 g
Slide13Cell
Analyzes
After treatment,
100 mL from cell suspensions
were plated into wells
of 96 well plates (each sample
was
assessed
in
quadruplicate)
After an
18
hour
recovery
(5% CO
2
,
37°C),
10 mL
of MTT
reagent
was added to each well and incubated (5% CO2, 37°C) for four hours.100 mL of detergent reagent was added to each well and samples were incubated (5% CO2, 37°C) for four additional hoursOD 590
was measured to detect MTT reduction to formazan dye (by mitochondrial enzymes) in order to assess
cytotoxicity
Slide14R
esults
Slide15O
D
59
5
n
m
1.2
1
0.8
0.6
0.4
0.2
0
Time=
0
Time=
1
Time=
2
Tim
T
e
im
=
e
3
in
T
h
i
o
m
u
e
rs
=
4 Time = 6
Time=
7
Time=
24
Control
BT549 experiment
with
cell left out of incubator
4.8.16
Slide1611.24.16
experiment
BT549
Basal
breast cancer
cells
Slide17Conclusive
results
0
.7
0
.6
0
.5
0
.4
0
.3
0
.2
0
.1
0
C
o
n
t
ro
l
833,
v=11
883,
v=10.5
833,
v=9
O
D
59
5
n
m
Slide180
0
.1
0
.2
0
.3
0
.4
0
.5
0
.6
0
.7
0
.8
293
control
293
833
293
833
O
D
59
5
n
m
BT549
and
HEK
203 control
cells
6.20.16,
v=12.5
Slide190
0
.2
0
.4
0
.6
0
.8
1
1
.2
1
.4
BT549
control
BT549
833
BT549
833
O
D
59
5
n
m
BT549
and
HEK
203 control
cells
6.20.16,
v=12.5
Slide2011.24.16
experimentBT549 Basal
breast cancer cells
Slide21Discussion
These
results help us
understand that if the intensity of the frequency
is lowered, more cells might die. The test conducted
at11.0 Volts
to
amplifier resulted
in killing
of
the
BT549
breast
cancer
cells
(approx.
51%)
in the waveguide.
Additionally,
the
test
conducted at
11.0V resulted on 30% of the Kidney Cells 293. This suggests that wave amplitudes must be selected carefully for tests, and that the healty kidney cells were much more resistant to the experiments. More tests of cytotoxicity/viability are underway.
Slide22Future
Research
Our future
goals
will
include alternating frequencies, voltage, and treatment duration
to
eradicate as
many
cells as possible.
We
will
also
investigate
how
to
specifically target the
cancer
cells
only
without
damaging the healthy cells. Additional cancer cell lines
will be investigated as well as more carefully designed experiments will be conducted. Possibility of caner cell death from thermos will be reduced.
Slide23R
eferences
1.
K
enir
y
,
M
.
an
d
R
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P
arsons,
T
he
r
o
l
e of PTEN signalingperturbations incancer and in targeted therapy. Oncogene, 2008. 27(41): p:5477-852. Markov,
M.S., Expanding use of pulsed electromagnetic field therapies.
Electromagn. Biol. Med, 2007. 26(3): p-257-74
3. Lsciacero, P., et al., The role of radiation therapy in vulvar cancer. Review ofthe current literature. Tumori. 2016: p..4. Mu,Y., et
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f
f
ec
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veness
an
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chemothera
p
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comb
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t
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cytokine-induced killer
cell
/dentritic cell-cytokine-induced
killer
cell
therapy
f
or
t
reat
m
ent
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f gast
r
i
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canc
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r
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C
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