Special Staining; spore and capsule PowerPoint Presentation, PPT - DocSlides

Download alexa-scheidler | 2017-06-19 | General Lab #4 . Medgar Evers College. Bio 261 Microbiology. Prof. Santos. Aim #1 exercise 14. Capsular staining. Capsule- extracellular layer composed of polysaccharides found in some bacteria that plays a role in immunity and adhesion.. ID: 561057

PowerPoint Special Staining; spore and capsule PowerPoint Presentation, PPT - DocSlides Slideshow

Only the spore is stained red with carbolfuchsin and the background is treated with Nigrosin to create contrast.


  • Views 31
Download this presentation

Special Staining; spore and capsule PowerPoint Presentation, PPT - DocSlides

Click below link (As may be) to download this presentation.

Download Note - The PPT/PDF document "Special Staining; spore and capsule Powe..." is the property of its rightful owner. Permission is granted to download and print the materials on this web site for personal, non-commercial use only, and to display it on your personal computer provided you do not modify the materials and that you retain all copyright notices contained in the materials. By downloading content from our website, you accept the terms of this agreement.

Presentations text content in Special Staining; spore and capsule PowerPoint Presentation, PPT - DocSlides

Slide1

Special Staining; spore and capsule

Lab #4

Medgar Evers College

Bio 261 Microbiology

Prof. Santos

Slide2

Slide3

Aim #1 exercise 14

Capsular staining

Capsule- extracellular layer composed of polysaccharides found in some bacteria that plays a role in immunity and adhesion.

It helps bacteria like

Streptococcus pneumonia

by preventing white blood cells from engulfing them and allowing them to invade the lungs.

It helps bacteria like

Streptococcus mutans

attach to the surface of teeth and cause dental plaque.

Slide4

Problems staining the capsule

1- fixing the cells after creating a smear will destroy the delicate capsule.

2- If cells are not fixed properly, they will wash off during staining, thus you will not see anything!

We avoid this by using a combined method of negative staining and a simple stain.

Slide5

procedure

1- we mix a couple of loopfuls of the

Klebsiella pneumonia

with India Ink. This will stain the background.

2- The ink suspension is spread over the slide and air dried.

3- GENTLY heat fix!

4- stain with crystal violet for one minute!

5- wash the crystal violet off with water

6- blot dry and look under microscope!

Slide6

Slide7

Aim #2 Exercise 16

Spore stain

Some species of bacteria belonging to the genera

Bacillus

and

Clostridia

produce tough protective structures called endospores.

Endospores are resting stages that allow the bacteria to resist harsh environmental conditions or treatments.

The spore contains a tough protein coat called the exosporium that allows it to form a barrier against all harsh conditions.

A spore is a very dehydrated structure (as much as 10 to 30 % less water than vegetative cell). The less water it has the more heat resistant.

Slide8

Due to nature of the spore, it is hard to stain them and get the stain to penetrate. But, using heat as the mordant allows the primary stain to penetrate the spore.

2 methods; Schaeffer-Fulton method and Dorner method

Slide9

Schaeffer- Fulton

Malachite green is the primary stain and stains the endospore greenSafranin is the counterstain and stains the vegetative cell pink.

Slide10

Dorner method

Only the spore is stained red with carbolfuchsin and the background is treated with Nigrosin to create contrast.

Recommended
Cultural Industries:
  • 21

Cultural Industries:

DEVELOPING GOOD
  • 24

DEVELOPING GOOD

Social Issues in Sport
  • 32

Social Issues in Sport

Reporting on Sports
  • 26

Reporting on Sports

Famous British Sportsmen and Sportswomen
  • 49

Famous British Sportsmen and Sportswomen

Sportsmanship Ideas
  • 24

Sportsmanship Ideas

Red Bird Fall Fires 2002
  • 16

Red Bird Fall Fires 2002

Living Well Spotlight
  • 20

Living Well Spotlight

Anoob Hamza Mohammed  |  1HM03EC006  |  VIII  ECE  |
  • 15

Anoob Hamza Mohammed | 1HM03EC006 | VIII ECE |

Report this Document.