In total 3 vials BSA includedrestriction enzymes are active in a 1X or 2X concentration Buffer Please refer to Buffer 33 mM Trisacetate pH 79 at 37C 10 mM magnesium acetate 66 mM potassium acetate ID: 871933
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1 1200 U Lot: ___ Expiry Date: _ï¯C...3'
1200 U Lot: ___ Expiry Date: _ï¯C...3' 3'...CïT C G A G5' Concentration: 10 U/µL Supplied with: 1 mL of 10X Buffer SacI 1 mL of 10X Buffer Tango In total 3 vials. BSA includedrestriction enzymes are active in a 1X or 2X concentration Buffer. Please refer to Buffer: 33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM magnesium acetate, 66 mM potassium acetate, nuc
2 lease-free water 16 µL 10X Buffer SacI 2
lease-free water 16 µL 10X Buffer SacI 2 µL DNA (0.5-1 µg/µL) 1 µL SacI 0.5-2 µL PCR reaction mixture 10 µL (about 1 µg of DNA)nuclease-free water 16 µL 10X Buffer SacI 2 µL SacI 1-2 µL SacIBTango2X Tango 50-100 20-50 0-20 0-20 50-100 20-50 CpG: may overlap no effect. Digestion of Agarose-embedded DNA complete digestion of 1 µg of agarose-embedded lambda Alw21I, Eco2
3 4I, SduI ï¬ï ïX174 pBR322 pUC57 pUC1
4I, SduI ï¬ï ïX174 pBR322 pUC57 pUC18/19pTZ19R/U M13mp18/19 2 0 0 1 1 1 1 GAGCTmC and insensitive to adenine methylation at GmAGCTC. AluI methyltransferase (AGmCT) can be used to block SacI.Supercoiled plasmids may require Overdigestion Assay with SacI (10 U/µg lambda DNA x 16 hours). The ligation and recleavage assay was replaced with LO trace nuclease and phosphatase
4 activities with sensitivity No detectabl
activities with sensitivity No detectable degradation of single-stranded or double-stranded labeled oligonucleotides occurred during PRODUCT USE LIMITATION The product was not tested for use in diagnostics or for drug Please refer to www.thermoscientific.com/onebio © 2012 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the ific Inc. and its subsidiaries