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1 Genet. Res., Camb. (1985) 46 pp. 353-35
Genet. Res., Camb. (1985) 46 pp. 353-35 ggWith 2 platesPrinted in Great BritainSHOR PAPEChromosom difference betwee substrain o thinbre 10 mous straiB E P EVANS* M D BURTENSHAW AN ILSE-DOR ADLER* Medical Research Council External Staff, Sir William Dunn D Y D L O D E O H D W K W W S V Z Z Z F D P E U L G J H R U J F R U H W H U P V K W W S V G R L R U J 6 ' R Z Q O R D G H G I U R P K W W S V Z Z Z F D P E U L G J H R U J F R U H , 3 D G G U H V V R Q $ X J D W V X E M H F W W R W K H & D P E U L G J H & R U H W H U P V R I X V H 35 E P EVANS M D BURTENSHA AN ILSE-DOR ADLE101/S (fo Selby wa derive fro 101/E b importatio t Oa Ridg froNeuherber i 1976Karyotype wer produce fo al fou 10 substrain an als fo tw C3H/Hsubstrains C3H/HeHOx (a offshoo o th Harwel C3H/H substrai sen t Oxfori 1971 an C3H/HeE fro Neuherberg Thes wer prepare fro air-drie preparationmad fro direc bone-marro (Ford 1966 withou usin a mitoti arrestan an witaqueou 0-5 % (w/v Potassiu chlorid a th hypotoni treatment mitoti cell werG-bande b a modificatio o a combine ASG/trypsi metho (Gallimor & Richardson1973) Suitabl sprea an G-bande cell wer photographe unde a x 10 oiimmersio lens Th chromosome wer cu ou fro th photographi print obtainean compare b visua inspection U t te print wer analyse fo eac substrainTh G-band wer identifie accordin t th standar idiogra (Nesbit & Francke1973 an th siz o th centromeri heterochromati region (whic ar synonymout th A G-bands compare b par referenc t publishe informatio (fo examplese Davisson 1981) Fo purpose o nomenclature thi regio ha bee designate b symbo He I i know t var i siz i u t five chro
2 mosom pair i som inbre i compare t th ac
mosom pair i som inbre i compare t th accepte 'normal whic i th siz o H region i thC57BL/6 strain Th variation i size followe b chromosom number ar expressea Hcs fo small He1 fo larg an Hcn fo normal i o th sam siz a thos i C57BL/6JUnfortunately th publishe informatio doe no encompas al th visibl H sizvariation betwee inbre strain sinc i i base o comparison o relativel grosdifference an thei departure fro th standar karyotype o th on inbre strain i ou experienc tha comparison mad betwee th chromosome o inbre strain revea man more som o the albei small difference tha ar suggeste b thexistin lis o centromeri heterochromati variant (se fo example Davisson 1981 anwhic canno b accommodate b th existin nomenclature I ou comparisons whav mad us o thes difference an describ H size a bein larg o smal relativt th chromosom pair withi th 10 an C3H/H substrain studied Althoug wstudie G-bande chromosome an di not i th strictes sense observ H regionbu th synonymou A G-ban regions i i mor convenien t refe t the a Hsince fo these strai variant hav bee recorde bu A ban variant ar nomentione i th standar idiogram3 RESULTApar fro th difference i th size o th A band (hereafte referre t a He)n othe G-ban patter difference wer observe i th compariso o karyotype froth fou 10 an th tw C3H/H substrains Withi th fou 10 substrains Hdifference wer observe i chromosom pair 1 2 7 8 1 an 1 bu sinc som othe wer subtl an sometime subjec t th possibilit o othe variatio resultingfo example fro technica difference betwee slides overal comparison betwee al substrain wer restricte t th mos extrem difference whic wer observe i 8 an 13 Th comparison sho (Plat 1 tha bot th 101/HOx an 101/Rsubstrain hav smal Hc bu larg Hcl wherea th 101/E an 101/S hav threverse larg Hc an smal Hcl3 I size thes ar mor aki t th H region o theschromosom pair fro
3 th tw C3H/H substrainsSince a leas wit
th tw C3H/H substrainsSince a leas wit respec t chromosom pair 8 an 13 101/HOx appeare t bth sam a 101/R1 an 101/E th sam a 101/Sl a mor detaile an controllecompariso o H size wa mad betwee al th chromosome fro karyotype o th101/HOxe 101/E an C3H/HeHOx substrains N consisten difference werobserve i te chromosom pair (pair 3 4 6 9 10 11 12 14 1 an 19 no i thY chromosome I thre pair (Pair 5 1 an 18 an i th X chromosome 101/HOxwa considere equivalen t 101/El bot differin fro C3H/HeH0xe I th D Y D L O D E O H D W K W W S V Z Z Z F D P E U L G J H R U J F R U H W H U P V K W W S V G R L R U J 6 ' R Z Q O R D G H G I U R P K W W S V Z Z Z F D P E U L G J H R U J F R U H , 3 D G G U H V V R Q $ X J D W V X E M H F W W R W K H & D P E U L G J H & R U H W H U P V R I X V H Genetical Research, Vol. 46 Part 3 Plat 1Chromosom pair101/Rit101/HOx! ! « }C3H/HeE £101/E k %I«fPLAT 1A compariso betwee H (arrowed size i chromosom pair 8 an 1 fro th fou 10 anth tw C3H/H substrainsE P EVAN AN OTHER (Facing p. 354 D Y D L O D E O H D W K W W S V Z Z Z F D P E U L G J H R U J F R U H W H U P V K W W S V G R L R U J 6 ' R Z Q O R D G H G I U R P K W W S V Z Z Z F D P E U L G J H R U J F R U H , 3 D G G U H V V R Q $ X J D W V X E M H F W W R W K H & D P E U L G J H & R U H W H U P V R I X V H Genetical Research, Vol. 46 Part 3 Plat 2Chromosom pair an th X2 5 7 8 1 1 1 1 X101/HOx101/E2 M • % • • |
4 | 1 * Z• • • f •C3H
| 1 * Z• • • f •C3H/HeHOx \PT.AT 2A compariso betwee H (arrowed size i chromosom pair 1 2 5 7 8 13 15 1 an 1an i th X chromosom fro tw o th 10 an on o th C3H/H substrains H size large ar indicate b a + sig place beneat th pair an i considere smallerb a — sign T th tw sign correspond th pair ar considere equivalentE. P. EVAN AN OTHER D Y D L O D E O H D W K W W S V Z Z Z F D P E U L G J H R U J F R U H W H U P V K W W S V G R L R U J 6 ' R Z Q O R D G H G I U R P K W W S V Z Z Z F D P E U L G J H R U J F R U H , 3 D G G U H V V R Q $ X J D W V X E M H F W W R W K H & D P E U L G J H & R U H W H U P V R I X V H Short paper 35remainin si pair (pair 1 2 7 8 1 an 17) 101/E wa considere mor equivalent C3H/HeH0x tha t 101/HOx (Plat 2)4 DISCUSSIOFro th geneti difference foun betwee 101/ an 101/E1 Wes et al. (1984conclude tha th mos likel sourc o sublin divergenc stemme fro th contaminatio o 101/E b anothe strai whic wa possibl C3H/He Ou observation oth karyotype confir thi conclusio an als sugges tha th substrai carrie a leastw chromosom pair ( an 13 an possibl a furthe fou (1 2 7 an 17) whic coulb o C3H/H originTh observation als suppor th contention o Wes et al. (1984 regardin thhistor o 101/H Sinc th karyotype o 101/ an 101/R appea identica withith limitation o ou study the mus represen th 'true 10 strai a originalldevelope b Dun an sen t Oa Ridg i 1947-8 Further th observation o thsam karyotyp marker i 101/E an i 101/S show a expecte fro th relativelrecen importatio o th latter tha the ar chromosomall relate an carr th sam chromosomesI i o interes t compar th geneti finding
5 o Wes et al. (1984 wit ou findings.The t
o Wes et al. (1984 wit ou findings.The teste seve mappe loci o whic on wa uninformativ sinc th sam allel presen i al thre substrains O th othe six two o chromosom 5 wer fount b th sam i 101/ an i 101/E bu differen i C3H/HeH Th remainin fouloci o chromosome 2 3 7 an 11 wer differen i th tw 10 substrain bu th sami 101/E a i C3H/HeH I ou chromosom study an assumin fro th evidencobtaine fro th karyotype tha th Oxfor substrain ar th sam a th Harwelones w conclude tha chromosom 5 wa equivalen i th tw 10 substrain budifferen i C3H/He Chromosome 2 an 7 wer equivalen i 101/E an i C3H/Hbu differen i 101/ an chromosome 3 an 1 wer no informativ sinc the ha size H region i al thre substrains Th geneti an chromosoma findings therefor compatibl fo th thre informativ chromosome an i suitabl variant available i woul b o interes t compar loc o th othe fou chromosom pair(1 8,1 an 17 whic w considere t b mor equivalen i 101/E t thos o C3H/Hrathe tha t thos o 101/HOxeTh C3H/H bu no th 10 strai i include i th lis o centromeri heterochromativariant i inbre strain (se Davisson 1981) I i possible however tha th latte hav simila variant t th liste 12 strai whic wa derive fro th sam sourc(se fo exampl Festing 1979) I th compariso t th norma standar o th Hregio size i th C57BL/6 strain th C3H/He i liste a havin Hcls, Hcl4s anHcl8s an th 12 a havin Hc8s an Hcl4s. W observe smal H i chromosome1 an 1 (Plat 2 an i chromosom 1 (no illustrated fro th C3H/HeHOxsubstrain I strai 101/HOxe w observe smal H i bot chromosome 8 (Plat 2 1 (no illustrated consisten wit i havin a commo origi wit strai 129 Strai101/E1 however ha smal H o chromosom 14 bu larg o norma size H ochromosom 8 (Plat 2) Thi provide furthe evidenc tha 101/E1 rathe tha 101/Hi th contaminate strainW ar gratefu t Dr Mar F Lyon J Peter an Joh Wes fo providin som o thmous mate
6 ria an als fo thei helpfu commentsREFERE
ria an als fo thei helpfu commentsREFERENCEDAVISSON M T (1981) Centromeri heterochromati variants I Genetic Variants and Strainsof the Laboratory Mouse (ed M C Green) pp 357-358 Stuttgar an Ne York Fischer D Y D L O D E O H D W K W W S V Z Z Z F D P E U L G J H R U J F R U H W H U P V K W W S V G R L R U J 6 ' R Z Q O R D G H G I U R P K W W S V Z Z Z F D P E U L G J H R U J F R U H , 3 D G G U H V V R Q $ X J D W V X E M H F W W R W K H & D P E U L G J H & R U H W H U P V R I X V H 35 E P EVANS M D BURTENSHA AN ILSE-DOB ADLBFBSTING,M F W.(1979) Inbred strains in Biomedical Research, p 138 Londo an BasingstokeMacMillanFORD C E (1966) Th us o chromosom markers I Tissue Grafting and Radiation (edH S Mickle an J F Loutit) pp 197-206 Ne York Academi PressGALLIMORE P H & RICHARDSON C R (1973) A improve bandin techniqu exemplifiei th karyotypi analysi o tw strain o rat Chromosoma 41 259-263NESBITT M N & FRANCKE U (1973) A syste o nomenclatur fo ban pattern o mouschromosomes Chromosoma 41 145-158WEST J D. PETERS J & LYON M F (1984) Geneti difference betwee tw substrain oth inbre 10 mous strain Genetical Research 44 343-346 D Y D L O D E O H D W K W W S V Z Z Z F D P E U L G J H R U J F R U H W H U P V K W W S V G R L R U J 6 ' R Z Q O R D G H G I U R P K W W S V Z Z Z F D P E U L G J H R U J F R U H , 3 D G G U H V V R Q $ X J D W V X E M H F W W R W K H & D P E U L G J H & R U H W H U P V R I X V H