Jared Alvarez Chris McGovern LiChong Xu Chris Siedlecki Gail Matters Introduction Pancreatic ductal adenocarcinoma is one of the deadliest forms of cancer Detection Treatment resistance ID: 1041342
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1. Targeted ICG-Nanobubbles for Detection of Pancreatic CancerJared Alvarez, Chris McGovern, Li-Chong Xu, Chris Siedlecki, Gail Matters
2. IntroductionPancreatic ductal adenocarcinoma is one of the deadliest forms of cancerDetectionTreatment resistancehttps://utswmed.org/medblog/pancreatic-cancer-nutrition/
3. Photoacoustic imaging is a promising biomedical toolHan, S. H. (2018). Review of Photoacoustic Imaging for Imaging-Guided Spinal Surgery. Neurospine, 15(4), 306-322. doi:10.14245/ns.1836206.103
4. HypothesisPhotoacoustic imaging will improve early detection of pancreatic cancer.Targeted PLGA nanobubbles will improve photoacoustic image contrast and sensitivity.My GoalOptimize synthesis of Indocyanine green (ICG) loaded PLGA nanobubblesQuantify ICG encapsulated in loaded PLGA nanobubbles
5. Synthesizing PLGA nanobubbles (NBs)CHCl2+PLGAPVAIsopropanol washCentrifugal washFiltrationSonicationSonicationDouble Emulsion
6. EmulsionsTwo normally immiscible liquids mixed through dispersionEmulsion within an emulsionWater/oil/water multiple emulsionWater in oil single emulsion
7. Synthesizing PLGA nanobubbles (NBs)CHCl2+PLGAPVAIsopropanol washCentrifugal washFiltrationSonicationSonicationDouble Emulsion
8. AFM Samples SamplesUnfilteredFiltered
9. Quantifying ICG Encapsulation
10. Quantifying ICG EncapsulationBatchAverage AbsorbanceCalculated ICG Concentration (µM)NB15 4.130.03045N/ANB15 ICG 4.250.3756243.5857
11. NIR Image of ICG Encapsulated NBsFree ICGPLGA NBsPLGA ICG NBs
12. Future directionsQuantifying aptamer attachmentZeta potential PicogreenQuantifying particle fluorescence lifetimeIn vitro testingIn vivo testing
13. AcknowledgementsMatters LabDr. Gail MattersChris McGovernLaura FelixDelphin MongaSiedledki LabDr. Li-Chong XuDr. Chris SiedledkiTEM CoreDr. Han ChenCollaboratorsDr. Sri-Rajasckhar KothapalliSumit AgrawalAjay DangiSTEP-UPDr. Bonnie Halpern-FelsherSheila McLaughlinMentorsTheresa SwensonDr. KC AngDr. Marnie HalpernThe STEP-UP HS program is supported by the National Institute of Diabetes and Digestive and Kidney Diseases of the National Institutes of Health Grant #: 2R25DK078382-12