Urinary tract infection (UTI) - PowerPoint Presentation

Slide1

Urinary tract infection (UTI)

Lec

. 2

Urinary Tract Infection (UTI)

is a bacterial infection that affects any part of the urinary tract. A urinary tract infection

occurs where bacteria and white blood cells are present in the urine of a patient with symptoms of infection of the urethra, urinary bladder, or the kidney.

The most common type of UTI is acute cystitis often referred to as a bladder infection. An infection of the upper urinary tract or kidney is known as

pyelonephritis

.

Bacteriuria

occurs when

bacteria

are present in the urine;

pyuria

occurs when

white blood cells

are present in the urine.

The

bacteria that cause urinary tract infections are usually of fecal origin (e.g.,

Escherichia coli

).

Etiology

The

most common cause of urinary tract infections (i.e.,

urethritis

, cystitis, and

pyelonephritis

) is

Escherichia coli

.

Staphylococcus

saprophyticus

is the second most common cause of these infections in

females between the ages of 13 and 40

.

In

complicated cases of urinary tract infections such as those resulting from anatomic obstructions or from catheterization, the most common causes are

E. coli

,

Klebsiella

pneumoniae

, Proteus mirabilis, Enterococcus

, and

Pseudomonas

aeruginosa

.

Manifestations

Urethritis

:

is an infection of the urethra that causes pain and discomfort during voiding (

dysuria

).

Cystitis:

is an infection of the urinary bladder. Signs and symptoms of cystitis include urgency and frequency of urination, voiding small volumes of urine, and supra pubic tenderness just before or immediately after voiding.

Pyelonephritis:

is

an infection of the urinary tract ascends the ureters to enter the kidneys, the signs and symptoms include fever, flank pain and tenderness, cost vertebral angle tenderness, nausea and vomiting.

Urine Culture

Urine

is the specimen most frequently submitted for culture. The most common sites of urinary tract infection (UTI) are the urinary bladder (cystitis) and the urethra. From these sites the infection may ascend into the

ureters

(

ureteritis

) and subsequently involve the kidney (

pyelonephritis

). Females are more prone to infection of the urinary tract than are males.

Factors

that increase female susceptibility to UTI:

Short length of the urethra

Urethral contamination by rectal pathogens

Vestibular colonization by pathogenic bacteria

Decreased urethral resistance after menopause

In both males and females, UTI may be asymptomatic, acute, or chronic.

-

Asymptomaic

infection can be diagnosed by culture.

-Acute UTI is more frequently seen in females of all ages; these patients are usually treated on an outpatient basis and are rarely admitted to hospital.

-Chronic UTI in both males and females of all ages is usually associated with an underlying disease (e.g.

pyelonephritis

, prostatic disease, or congenital anomaly of the genitourinary tract) and these patients are most often hospitalized.

Since urine itself is a good culture medium, all specimens should be processed by the laboratory within 2 hours of collection, or be kept refrigerated at 4C until delivery to the laboratory and processed no longer than 18 hours after collection

.

The examination procedure includes the following steps:

1.

Examination of a Gram-stained smear

. ( for the presence or absence of bacteria,

polymorphonuclear

leukocytes, and

squamous

epithelial cells). One or more bacterial cells per oil-immersion field usually implies that there are 10

5

or more bacteria per milliliter in the specimen. The presence of one or more leukocytes per oil-immersion field is a further indication of UTI

.

2.

A screening test for significant

bacteriuria

. The absence of leukocytes and bacteria in a Gram-stained smear of a clean catch urine sample prepared as described above is good evidence that the urine

is not infected

.

A

urine specimen that is “

negative

” on careful examination of the Gram-stained smear does not need to be cultured.

An alternative simple and effective

screening test is the test strip for leukocyte esterase/nitrate reduction.

The strip is dipped into the urine specimen as instructed in the package literature.

Any pink

colour

is a positive

reaction indicating the presence of leukocyte esterase and/or bacteria in excess of 10

5

per ml. Urine samples that are positive in the screening test should be cultured as soon as possible to prevent possible overgrowth by no significant bacteria.

If the strip does not develop a

pink

colour

it is interpreted as a negative screening test, is so reported, and no culture is indicated.

3. A definitive culture for urine specimens found to be positive in the screening test.

4.

Susceptibility tests

on clinically significant bacterial isolates should only be performed on well-isolated colonies of similar appearance. Susceptibility tests are generally more important on cultures obtained from patients who are hospitalized or have

a

history of recurring UTI.

Procedure of urine culture

The recommended procedure uses a calibrated plastic or metal loop to transfer 1μl of urine to the culture medium.

1. Shake the urine gently, then tip it to a slant and with a 1μl inoculating loop touch the surface so that the urine is sucked up into the loop. Never dip the loop into the urine.

2. Deposit 1μl of the urine on a blood agar plate and streak the plate.

3. Inoculate the

MacConkey

agar in the same manner.

4. Incubate the plates overnight at 35 – 37Cº.

Culture media for urine culture

The

urine samples routinely culture on conventional media (

Blood agar and

MacConkey

agar

).

Now urine samples culture

on

CLED

agar (

Cysteine

lactose electrolyte deficient agar):

CLED agar is now used by most laboratories to isolate urinary pathogens

because it gives consistent results and allows the growth of both Gram negative and Gram positive pathogens. It inhibit

Proteus

swarming and accommodate common pathogens

(the indicator in CLED agar is

bromothymol

blue and therefore lactose fermenting colonies appear yellow).

Chromogenic

media

Antibiotic susceptibility test

Sensitivity

(susceptibility) testing is used to select effective antimicrobial drugs. The standardized disc-diffusion method (

Kirby–Bauer)

is used.

Disc diffusion techniques are used by most laboratories to test routinely for antimicrobial sensitivity.

A disc of blotting paper is impregnated with a known volume and appropriate concentration of an antimicrobial, and this is placed on a plate of sensitivity testing agar (Mueller–Hinton agar for most bacteria and blood agar for some bacteria) which uniformly inoculated with the test organism.

The antimicrobial diffuses from the disc into the medium and the growth of the test organism is inhibited. Strains sensitive to the antimicrobial are inhibited at a distance from the disc whereas resistant strains have smaller zones of inhibition or grow up to edge of the disc.

All strains of streptococci should be tested on blood agar for susceptibility.

All Gram-negative rods and staphylococci were tested on Mueller Hinton agar for susceptibility.

Strains of

Hemophilus

and

Neisseria

should be tested for susceptibility using Chocolate agar

.

Kidney function test

Serum

Creatinine

Creatinine

is a waste product that comes from meat protein in the diet and also comes from the normal wear and tear on muscles of the body.

Creatinine

levels

in the blood can vary, and each laboratory has its own normal range. In many labs the normal range is 0.6 to 1.2 mg/dl. Higher levels may be a sign that the kidneys are not working properly.

As

kidney disease

progresses, the level of

creatinine

in the blood increases

.

Blood Urea Nitrogen

(BUN)

Urea nitrogen is produced from the breakdown of food protein. A normal BUN level is between 7 and 20 mg/dl. As kidney function decreases, the BUN level increases.

Thank you