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HLA - Tissue Typing  Laboratory HLA - Tissue Typing  Laboratory

HLA - Tissue Typing Laboratory - PowerPoint Presentation

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HLA - Tissue Typing Laboratory - PPT Presentation

Department of Pathology amp Laboratory Medicine Human Leukocyte Antigen HLA complex allows the body to distinguish between its own proteins and the proteins of foreign invaders Located on chromosome 6 in humans the study of HLA is essential in organ transplantation ID: 744023

donors transplant hla donor transplant donors donor hla organ lab patients recipients 2015 medicine hours tubes marrow renal top

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HLA - Tissue Typing Laboratory Department of Pathology & Laboratory Medicine

Human Leukocyte Antigen (HLA) complex allows the body to distinguish between its own proteins and the proteins of foreign invaders. Located on chromosome 6 in humans, the study of HLA is essential in organ transplantation.

The HLA lab at UR Medicine is made up of one Faculty Director, one Supervisor, six Medical Technologist Specialists and one Support Tech. The staff works closely with the Renal, Heart and Bone Marrow transplant teams, as well as with the Organ Procurement team with Finger Lakes Donor Recovery Network. The Technologists are on-call to facilitate deceased donor organ transplant24 hours a day, 365 days a year. The workup for an average deceased donor takes 8 hours to complete and technologists mustreport immediately when paged.

Angela Busacco:

Supervisor

Dr. Myra Coppage:

Lab Director

Top row:

Jonathan Hoffmann, Karen Kryder, Li Zhang

Bottom row

: Tia LaBarge, Ricardo Rivera, Will

Femec

Not Pictured: Nancy Mistretta

Acceptable Samples:

Red top tubes and Serum Separator Tubes (SST)-

Used to screen transplant recipients for antibody before and after transplant.

Lavender and Yellow

(ACD) top

tubes

- Used for genetic typing of Donors and Recipients, in addition, to screening for disease

.

Green top

(Sodium Heparin tubes)-

Used for screening of potential Bone Marrow Sibling donors and cross matching for living related donors in renal transplant.

Total by Quarter

Renal

Heart

Liver

and Kidney

HLA

work up,

DNU *

Quarter 1

4

3

0

7Quarter 27307Quarter 3145210Quarter 411409

NYFL Deceased Donor Solid Organ Transplants 2015

*DNU-Donor not used

Active Listed Renal patients in NYFL

: 247

Active Listed Heart patients in NYFL: 18

Organ Number of PatientsKidney349Heart24Liver48Pancreas4Kidney/Pancreas11Liver/Kidney2

Current UNOS Waitlist:Solid Organ Transplant at UR Medicine

~300-400 active donors

Donors are screened using a virtual crossmatch to find suitable recipientsCrossmatching can be performed usingfrozen lymphocytes when necessaryLongest Transplant Chain was 34 Donor/Recipients over a 3 month period.UR Medicine has facilitated 10 transplants through the NKR. Most NKR transplants are due to ABO incompatibility between donor and recipients, but 4 were performed due to high reactive antibody.

National Kidney Registry

Located 2-3670

Hours of Operation: 7am-5pmHLA tech on-call 24 hours /day

In 2015, the HLA lab matched 70 patients

from both related and unrelated donors. (35 related/35 unrelated)Average age of patient at time of transplant is 45.2 years old.At 40%, Acute Myeloid Leukemia was the most common diagnosis in patients receiving a transplant in 2015.The HLA lab tests siblings and parents to find acceptable matches. They also test donors from the National Marrow Donor Program (NMDP) and donors from all over the world. Typing a matching donor costs at least $10,000.

Bone Marrow Transplant

Accessioned

samples 2015 = 5936Tests performed 2015= 15,495

The HLA lab does more than type and match transplant Recipients and Donors. We also test for diseases associations…

Reactive Arthritis

Juvenile Rheumatoid Arthritis

Bechet's Disease

Celiac Disease

Ankylosing Spondylitis

Birdshot

Chorioretinopathy

Lupus

Drug

Sensitivity in HIV Patients

Narcolepsy

Sjogren’s Syndrome