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Clontech Laboratories Inc


Vector MapA Takara Bio Company1290 Terra Bella Avenue Mountain View CA 94043 USAUS Technical Support techclontechcomUnited States/Canada8006622566Asia Pacific16509197300Europe330139046880Japan81077543

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Document on Subject : "Clontech Laboratories Inc"— Transcript:

1 Vector Map Clontech Laboratories,
Vector Map Clontech Laboratories, Inc. A Takara Bio Company 1290 Terra Bella Avenue, Mountain View, CA 94043, USA U.S. Technical Support: tech@clontech.com United States/Canada 800.662.2566 Asia Pacific +1.650.919.7300 Europe +33.(0)1.3904.6880 Japan +81.(0)77.543.6116 Page 1 of 3 ( 020 5 14 ) pLVX - TetOne - Puro Vector Set Catalog No(s). 63184 9 (Not sold separately) Sold as a part of 63184 7 Figure 1 . pLVX - TetOne - Puro vector map and multiple cloning site. Vector Map Cat. No. 63184 9 pLVX - TetOne - Puro Vector Set (Not sold separately) Sold as a part of Cat. Nos. 63184 7 ( 020 5 14 ) Page 2 of 3 Location of Features ( pLVX - TetOne - Puro Vector )  5’ LTR (5’ long terminal repeat): 1 – 635  Ψ (packaging signal): 681 – 806  RRE (r ev - response element): 130 3 – 1536  cPPT/CTS (central polypurine tract/central termination sequenc e): 2028 – 2144  SV40 poly(A) signal: 2187 – 2321  MCS: 2496 – 2527  P TRE3G S ( TRE3G S promoter): 2528 – 2892  P h PGK ( human phosphoglycerate kinase 1 promoter): 2912 – 3422  Tet - On 3G (transactivator gene): 3441 – 4187  P SV40 (SV40 promoter): 4198 – 4527  PuroR (puromycin resistance gene): 4536 – 5135  WPRE (woodchuck hepati

2 tis virus posttranscriptional regulatory
tis virus posttranscriptional regulatory element): 5149 – 5737  3' LTR (3’ long terminal repeat): 5945 – 6578  Ori ( high - copy - number ColE1/pMB1/pBR322/pUC origin of replication ): 7109 – 7694  Amp R (ampicillin resis tance gene): 7865 – 8725  Amp R promoter: 8726 – 8830 (complementary) Figure 2 . pLVX - TetOne - Puro - Luc Control Vector map. Vector Map Cat. No. 63184 9 pLVX - TetOne - Puro Vector Set (Not sold separately) Sold as a part of Cat. Nos. 63184 7 ( 020 5 14 ) Page 3 of 3 Location of Features ( pLVX - TetOne - Puro - Luc Control Vector )  5’ LTR (5’ long terminal repeat): 1 – 63 4  Ψ (packaging signal): 68 1 – 8 06  RRE (R ev - response element): 130 3 – 1536  cPPT/CTS (central polypurine tract/central termination sequence): 2028 – 2144  SV40 poly(A) signal: 2187 – 2321  F irefly luciferase: 25 2 2 – 41 4 7  P TRE3G S ( TRE3G S promoter): 41 8 1 – 45 4 5  P h PGK ( human phosphoglycerate kinase 1 promoter): 45 6 5 – 50 7 5  Tet - On 3G (transactivator gene): 50 9 4 – 58 4 0  P SV40 (SV40 promoter): 5851 – 6180  PuroR (puromycin resistance gene): 6189 – 6788  WPRE (woodchuck hepatitis virus posttranscriptional regulatory element): 6802 – 7390 

3 3' LTR (3’ long terminal repe
3' LTR (3’ long terminal repeat): 7598 – 8231  Ori ( high - copy - number ColE1/pMB1/pBR322/pUC origin of replication ): 8762 – 9347  Amp R (ampicillin resistance gene): 9518 – 10,378 (complementary)  Amp R promoter: 10,379 – 10,483 (complementary) Notice to Purchaser Clontech products are to be used for research purposes only. They may not be used for any other purpose, including, but not limited to , use in drugs, in vitro diagnostic purposes, therapeutics, or in humans. Clontech products may not be transferred to thi rd parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without prior written approval of Clontech Laboratories, Inc. Your use of this product is also subject to compliance with the licen sing requirements described on the products web page at http://www.clontech.com . It is your responsibility to review, understand and adhere to any restrictions imposed by these statements. Clontech, the Clontech log o, and Tet - On are trademarks of Clontech Laboratories, Inc. All other marks are the property of their respective owners. Certain trademarks may not be registered in all jurisdictions. Clontech is a Takara Bio Company. 201 4 Clontech Laboratories, Inc. This document has been reviewed and approved by the Clontech Quality Assurance Departmen