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Vector Map


Clontech Laboratories Inc A Takara Bio Company1290 Terra Bella Avenue Mountain View CA 94043 USAUS Technical Support techclontechcomUnited States/Canada8006622566Asia Pacific16509197300Europe330139046

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Document on Subject : "Vector Map"— Transcript:

1 Vector Map Clontech Laboratories,
Vector Map Clontech Laboratories, Inc. A Takara Bio Company 1290 Terra Bella Avenue, Mountain View, CA 94043, USA U.S. Technical Support: tech@clontech.com United States/Canada 800.662.2566 Asia Pacific +1.650.919.7300 Europe +33.(0)1.3904.6880 Japan +81.(0)77.543.6116 Page 1 of 3 ( 0415 14 ) p RetroX - TetOne - Puro Vector Set Catalog No(s). 63430 9 (Not sold separately) Sold as a part of 63430 7 Figure 1 . p RetroX - TetOne - Puro V ector map and multiple cloning site. Vector Map Cat. No. 63430 9 p RetroX - TetOne - Puro Vector Set (Not sold separately) Sold as a part of Cat. Nos. 63430 7 ( 0415 14 ) Page 2 of 3 Location of Features (pRetroX - TetOne - Puro Vector)  5’ LTR (5’ long terminal repeat): 376 – 911  Ψ (packaging signal): 974 – 1331  SV40 poly (A) signal: 2298 – 2432  MCS: 2607 – 2638  P TRE3GS (TRE3GS promoter): 2639 – 3003  P hPGK (human phosphoglycerate kinase 1 promoter): 3023 – 3533  Tet - On 3G (transactivator gene): 3552 – 4298  SV40 promoter: 4316 – 4645  Puro R ( puromycin resistance gene): 4654 – 5253  WPRE (woodchuck hepatitis virus

2 posttranscriptional regulatory element):
posttranscriptional regulatory element): 5271 – 5679  3' LTR (del in U3): 5800 – 6047  Amp R promoter: 7325 – 7429  Amp R (ampicillin resistance gene): 7430 – 8290  ori (high - copy - number ColE1/pMB1/pBR322/pUC origin of replication): 8461 – 9049 Figure 2 . pRetroX - TetOne - Puro - Luc Control Vector map. Vector Map Cat. No. 63430 9 p RetroX - TetOne - Puro Vector Set (Not sold separately) Sold as a part of Cat. Nos. 63430 7 ( 0415 14 ) Page 3 of 3 Location of Features (pRetroX - TetOne - Puro - Luc Control Vector)  5’ LTR (5’ long terminal repeat): 376 – 911  Ψ (packaging signal): 974 – 1331  SV40 poly (A) signal: 2298 – 2432  Firefly luciferase : 26 13 – 4265  P TRE3GS (TRE3GS promoter): 4275 – 4639  P hPGK (human phosphoglycerate kinas e 1 promoter): 4659 – 5169  Tet - On 3G (transactivator gene): 5188 – 5934  SV40 promoter: 5952 – 6281  Puro R ( puromycin resistance gene): 6290 – 6889  WPRE (woodchuck hepatitis virus posttranscriptional regulatory element): 6907 – 7315  3' LTR (del in U3): 7436 – 7683  Amp R promoter: 8961 – 9065

3  Amp R (ampicillin resistance g
 Amp R (ampicillin resistance gene): 9066 – 9926  ori (high - copy - number ColE1/pMB1/pBR322/pUC origin of replication): 10097 – 10685 Notice to Purchaser Clontech products are to be used for research purposes only. They may not be used for any other purpose, including, but not limited to , use in drugs, in vitro diagnostic purposes, therapeutics, or in humans. Clontech products may not be transferred to third partie s, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without prior written approval of Clontech Laboratories, Inc. Your use of this product is also subject to compliance with the licensing requ irements described on the products web page at http://www.clontech.com . It is your responsibility to review, understand and adhere to any restrictions imposed by these statements. Clontech, the Clontech logo, and Te t - On are trademarks of Clontech Laboratories, Inc. All other marks are the property of their respective owners. Certain trademarks may not be registered in all jurisdictions. Clontech is a Takara Bio Company. 201 4 Clontech Laboratories, Inc. This document has been reviewed and approved by the Clontech Quality Assurance Departmen