PPT-Simultaneous Clustering of Multiple Gene Expression

and Physical Interaction Datasets Manikandan Narayanan Adrian Vetta Eric E Schadt Jun Zhu PLoS Computational Biology 2010 Presented by Tal Saiag Seminar in Algorithmic Challenges in Analyzing Big Data in Biology and . The problem to be solved (an example). Hauf, J., Zimmermann, F.K., M. ü. ller, S., 2000. Simultaneous genomic over expression of seven glycolytic enzymes in the yeast Saccharomyces cerevisiae. Ezyme. Microbiol. Technol.. Vibrio cholerae . HHMI 2011. Sara Fassio. Dr. Claudia H. ä. se. Dr. Yusuke Minato . Cholera. Infection of small intestine . Causes severe diarrhea and electrolyte loss. 3-5 million cases a year. 100,000- 120,000 deaths per year . Wounds. Clinical . Best Practices Track. Sandra J. Berriman, Ph.D. . . Kathryn . E. Davis, Ph.D. .. Vice President, Clinical . Affairs Adjunct . What does the operon model attempt to explain?. the coordinated control of gene expression . in bacteria. bacterial resistance to antibiotics. how genes move between homologous regions of DNA. the mechanism of viral attachment to a host cell. University of Notre Dame. Kristin Hager, Associate Teaching Professor, Biological Sciences. Joseph O’Tousa, Professor, Biological Sciences. WH Freeman. Marc Mazzoni, Senior Editor, Life Sciences. Elaine Palucki, Editor, Adjunct Assistant Professor Biology, Brooklyn College. Matthew Thompson, UF. matthewbot@ufl.edu. Prolific Authors. Important Papers. Prolific Institutions. Title. Year. Times Cited. Institutions or. Organizations. Simultaneous map building and localization for an autonomous mobile. Lecture outline. Distance/Similarity between data objects. Data objects as geometric data points. Clustering problems and algorithms . K-means. K-median. K-center. What is clustering?. A . grouping. of data objects such that the objects . 1. Mark Stamp. K-Means for Malware Classification. Clustering Applications. 2. Chinmayee. . Annachhatre. Mark Stamp. Quest for the Holy . Grail. Holy Grail of malware research is to detect previously unseen malware. Gene Expression. Gene Expression. Transcription. Splicing. Polyadenylation. mRNA Stability. Translation. Protein Stability. Controls on Protein Levels. Transcription Control - Prokaryotic Promoter. Transcription Control - Prokaryotic Promoter. Produces a set of . nested clusters . organized as a hierarchical tree. Can be visualized as a . dendrogram. A . tree-like . diagram that records the sequences of merges or splits. Strengths of Hierarchical Clustering. Log. 2. transformation. Row centering and normalization. Filtering. Log. 2. Transformation. Log. 2. -transformation makes sure that the noise is independent of the mean and similar differences have the same meaning along the dynamic range of the values.. We performed a genome-wide analysis of gene expressionto identify germline- and sex-regulated genes.Using mutants that cause defects in germ cell proliferationor gametogenesis, we identiÞed sets of g Randomization tests. Cluster Validity . All clustering algorithms provided with a set of points output a clustering. How . to evaluate the “goodness” of the resulting clusters?. Tricky because . Pearley Chinta and Juliet V. Spencer . Abstract. Methods. Results. Conclusions. HCMV is a widespread pathogen in the general population and can cause severe disease in immune-compromised hosts. HCMV manipulates immune responses in several ways, one of which includes encoding genes with homology to host chemokine receptors. HCMV US27 encodes a chemokine-like receptor that stimulates host gene expression. While, no chemokine ligand has been identified for US27, it is constitutively active. US27 stimulates the gene expression of antioxidant response element (ARE) regulated genes by activation of the transcription factor nuclear respiratory factor 1 (NRF-1). The goal of this project is to identify specific host and viral genes that are regulated by US27. Increased expression of antioxidant genes is likely to benefit virus infection and enable more progeny virus to be produced. Thus, a better understanding of the US27 function has the potential to lead to the development of novel antiviral therapies necessary to treat HCMV infection. .