Rajani Associate ProfessorMicrobiology Career Institute of Medical Sciences and Hospital Lucknow List of Bacteria Legionella pneumophila Gardenella vaginalis HACEK Streptobacillus ID: 1009107
Download Presentation The PPT/PDF document "Miscellaneous bacteria Dr Monika" is the property of its rightful owner. Permission is granted to download and print the materials on this web site for personal, non-commercial use only, and to display it on your personal computer provided you do not modify the materials and that you retain all copyright notices contained in the materials. By downloading content from our website, you accept the terms of this agreement.
1. Miscellaneous bacteriaDr Monika RajaniAssociate Professor,MicrobiologyCareer Institute of Medical Sciences and HospitalLucknow
2. List of BacteriaLegionella pneumophilaGardenella vaginalis HACEKStreptobacillus moniliformisSpirillum minusKlebsiella granulomatis
3. Legionella PneumophilaLegionnaires ds: new illness in 1976 in PhiladelphiaFever, cough and chest pain, pneumonia, fatal
4. Legionella pneumophilaGNCB, motile with polar flagellaBest visualised by Silver impregnation methods DFA stainingCulture :BCYE-Buffered charcoal yeast extract agar
5. Epidemiology Widely distributed in natural water sources like stagnant waters ,mud and hot springsHere Nutritional requirements are provided by algae in water sourcesCan also survive and multiply in free living amoebaeCan amplify in artificial aquatic environments which serve as amplifiers
6. Mode of infectionInhalation of aerosols from AC, cooling towers, shower heads acting as disseminatorsAerosolised legionellae carried to long distances and survive longerNo carrier state, no animal reservoir, no human to human transmissionRF: Smoking, alcohol, age
7. Mode of infection
8. Pathogenicity - LegionellosisCausative organism: Legionella pneumophilaPresentation: Legionnairres disease and Pontaic feverMOI: Inhalation of aerosols lead to entry in alveoli where they multiply within monocytes and macrophagesBeing intracellular only CMI Is effective for recoverySpread : occures by bloodstream, lymphatics
9.
10. Clinical presentation-Legionnaires diseaseEpidemic or sporadicIP=2-10 DAYSFever ,cough, dyspneaPneumoniaRespiratory failure and shock
11. Clinical presentation-pontaic fever Milder,non fatal,influenza life illnessFever,chills,myalgia
12. Lab diagnosisSample: sputum, bronchial aspirate, biopsyMicroscopy: DFA Culture: BCYE mediaSerology: Latex agglutination –detection of Ag in urineELISA
13.
14. SUMMARY
15. Gardnella vaginalisBacteria: GNR, NM, pleomorphicDisease: bacterial vaginosis
16. Disease: Bacterial vaginosisRaised vaginal pH>4.5fowl smelling dischargePresence of clue cellsNugents scoreMetronidazole is DOC
17. Clue cells Vaginal epithelial cells with their surface studded with numerous small bacteria.Clue cells are also seen in infections by anaerobic bacteria called Mobilincus
18. Clue cellsClue cells are single most reliable indicators of bacterial vaginosis
19. Nugents score The diagnosis of bacterial vaginosis is made on microscopic criteria of gram smear of high vaginal swab using the Nugents score A score of 7 to 10 is consistent with bacterial vaginosis without culture.
20.
21. All HACEK members are fastidious Gram-negative bacteria associated with Infective Endocarditis
22.
23. SPREADthey spread hematogenously following introduction into the blood during tooth brushing, dental cleanings, or subsequent to oral diseases such as periodontitis.
24. Clinical Identification of HACEK organismsPreviously, HACEK organisms were implicated in endocarditis from which no pathogen could be isolated (so called “culture-negative endocarditis”) This was attributed to slow growth in old formulations of blood culture bottles On Extended incubation (>5 days) the presence of these organisms was suspected.modern blood culture instruments reliably detect HACEK organisms within a 5-day incubation period.
25. ISOLATIONautomated blood culture systems (BACTEC) are better than manual cultureoften growing better on chocolate agar than on blood agar.MALDI-TOF is emerging as a potential identification technology for the HACEK group.
26. TREATMENTPENICILLINS CEPHALOSPORINS
27. Streptobacillus moniliformis and Spirillum minus
28. Both these bacteria are natural parasites of rodents and cause RAT BITE FEVER
29. STREPTOBACILLUS MONILIFORMISNatural parasite of rodentsDisease : Rat Bite Fever(RBF)Bacteria: GNR,pleomorphicGrows as tangled chain of rods
30. Amphitrichous flagella
31. Rat bite fever MOI: rat bite Consumption of raw milk or water contaminated by rats
32. RAT BITE FEVERFirst observed in Haverhill USAHAVERHILL FEVER Erythema arthriticum epidemicum
33. Clinical presentationAbrupt onset 2-10 days after exposureFever ,headache,myalgiaPetechial rasharthritis
34. LAB DIAGNOSISMicroscopic examination of blood and exudates from lesionStreptobacillus can be cultured on BA,CASpirillum isolated by intraperitoneal inoculation in mice serologyTreatmentOral penicillin doxycycline
35. Klebsiella granulomatis Formerly known as donovania granulomatis and Calymmatobacterium granulomatisDisease: DONOVANOSIS or Granuloma inguinale
36. Donovanosis Venereal diseasePainless papule on genitaliaShaft of penis,labia or perinium involvedLeads to slowly progressive autoinoculable ulcerChronicity
37. Lab diagnosisDemonstration of Donovan bodies in Wright Giemsa stained impression smears from the lesionsSafety pin appearance
38. Donovan BodiesRounded coccobacilli ,1-2 um in size within cystic spaces in large mononuclear cellsThe coccobacilli appear as CLOSED SAFETY PIN APPEARANCE In stained smears due to BIPOLAR condensation of chromatin
39. Important questionsShort notesHelicobacterAntibiotic associated diarrheaLegionella –atypical pneumoniaOral/vivaDonovan bodiesClue cellsrat bite feverUrea breath testDifferent motility and flagellar arrangement of bacteria
40. Thank you